Catalog No.
PXX05201
Species reactivity
Streptococcus pyogenes
Host species
Rabbit
Isotype
IgG
Clonality
Polyclonal
Immunogen
E. coli - derived recombinant Streptococcus pyogenes EndoS (Glu37-Lys995).
Tested applications
ELISA: 1:4000-1:8000, IHC: 1:50-1:200, WB: 1:1000-1:4000
Target
Endo-beta-N-acetylglucosaminidase
Concentration
1.26 mg/ml
Purification
Purified by antigen affinity column.
Accession
Q9APG4
Applications
ELISA, IHC, WB
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
The IgG-specific endoglycosidases EndoS and EndoS2 are distinguished by conformation and antibody recognition., PMID:38569940
Immunomodulating Enzymes from Streptococcus pyogenes-In Pathogenesis, as Biotechnological Tools, and as Biological Drugs., PMID:38258026
Pathogen-driven degradation of endogenous and therapeutic antibodies during streptococcal infections., PMID:37872209
Mechanism of antibody-specific deglycosylation and immune evasion by Streptococcal IgG-specific endoglycosidases., PMID:36973249
Extensive substrate recognition by the streptococcal antibody-degrading enzymes IdeS and EndoS., PMID:36528711
Desensitization using imlifidase and EndoS enables chimerism induction in allosensitized recipient mice., PMID:32185855
Streptococcal Enzymes as Precision Tools Against Pathogenic IgG Autoantibodies in Small Vessel Vasculitis., PMID:31616410
Structural insights into the mechanisms and specificities of IgG-active endoglycosidases., PMID:31172182
Streptococcus pyogenes evades adaptive immunity through specific IgG glycan hydrolysis., PMID:31092533
Endoglycosidase S Enables a Highly Simplified Clinical Chemistry Procedure for Direct Assessment of Serum IgG Undergalactosylation in Chronic Inflammatory Disease., PMID:30190373
Generation and Comparative Kinetic Analysis of New Glycosynthase Mutants from Streptococcus pyogenes Endoglycosidases for Antibody Glycoengineering., PMID:30102520
Streptococcal Endo-β-N-Acetylglucosaminidase Suppresses Antibody-Mediated Inflammation In Vivo., PMID:30061892
Structural basis for the recognition of complex-type N-glycans by Endoglycosidase S., PMID:29760474
Generation of efficient mutants of endoglycosidase from Streptococcus pyogenes and their application in a novel one-pot transglycosylation reaction for antibody modification., PMID:29474426
One-pot enzymatic glycan remodeling of a therapeutic monoclonal antibody by endoglycosidase S (Endo-S) from Streptococcus pyogenes., PMID:28789910
A facile synthesis of a complex type N-glycan thiazoline as an effective inhibitor against the antibody-deactivating endo-β-N-acetylglucosaminidases., PMID:30930529
Toward Clinical use of the IgG Specific Enzymes IdeS and EndoS against Antibody-Mediated Diseases., PMID:27914091
In vivo enzymatic modulation of IgG antibodies prevents immune complex-dependent skin injury., PMID:27512946
Glycosynthase Mutants of Endoglycosidase S2 Show Potent Transglycosylation Activity and Remarkably Relaxed Substrate Specificity for Antibody Glycosylation Remodeling., PMID:27288408
EndoSd: an IgG glycan hydrolyzing enzyme in Streptococcus dysgalactiae subspecies dysgalactiae., PMID:27200457
EndoS and EndoS2 hydrolyze Fc-glycans on therapeutic antibodies with different glycoform selectivity and can be used for rapid quantification of high-mannose glycans., PMID:26156869
Relative quantitation of glycopeptides based on stable isotope labeling using MALDI-TOF MS., PMID:25010467
Crystal structure of Streptococcus pyogenes EndoS, an immunomodulatory endoglycosidase specific for human IgG antibodies., PMID:24753590
Chemoenzymatic Fc glycosylation via engineered aldehyde tags., PMID:24702330
Fragments of bacterial endoglycosidase s and immunoglobulin g reveal subdomains of each that contribute to deglycosylation., PMID:24668806
Liquid-liquid diffusion crystallization improves the X-ray diffraction of EndoS, an endo-β-N-acetylglucosaminidase from Streptococcus pyogenes with activity on human IgG., PMID:24316841
EndoS2 is a unique and conserved enzyme of serotype M49 group A Streptococcus that hydrolyses N-linked glycans on IgG and α1-acid glycoprotein., PMID:23865566
Dominant suppression of inflammation by glycan-hydrolyzed IgG., PMID:23671108
Enzymatic deglycosylation converts pathogenic neuromyelitis optica anti-aquaporin-4 immunoglobulin G into therapeutic antibody., PMID:23055279
Chemoenzymatic glycoengineering of intact IgG antibodies for gain of functions., PMID:22747414
Enzymatic autoantibody glycan hydrolysis alleviates autoimmunity against type VII collagen., PMID:22652418
Antiphagocytic function of an IgG glycosyl hydrolase from Streptococcus equi subsp. equi and its use as a vaccine component., PMID:22615244
An endoglycosidase with alternative glycan specificity allows broadened glycoprotein remodelling., PMID:22551167
Selective deactivation of serum IgG: a general strategy for the enhancement of monoclonal antibody receptor interactions., PMID:22484364
IgG glycan hydrolysis by endoglycosidase S diminishes the proinflammatory properties of immune complexes from patients with systemic lupus erythematosus: a possible new treatment?, PMID:22392566
Study of the IgG endoglycosidase EndoS in group A streptococcal phagocyte resistance and virulence., PMID:21619648
IgG glycan hydrolysis attenuates ANCA-mediated glomerulonephritis., PMID:20448018
The IgG-specific endoglycosidase EndoS inhibits both cellular and complement-mediated autoimmune hemolysis., PMID:20357243
Successful treatment of experimental glomerulonephritis with IdeS and EndoS, IgG-degrading streptococcal enzymes., PMID:20219834
Sugar-free antibodies--the bacterial solution to autoimmunity?, PMID:19758213
In vivo enzymatic modulation of IgG glycosylation inhibits autoimmune disease in an IgG subclass-dependent manner., PMID:18815375
IgG glycan hydrolysis by a bacterial enzyme as a therapy against autoimmune conditions., PMID:18332429
Human IgG/Fc gamma R interactions are modulated by streptococcal IgG glycan hydrolysis., PMID:18183294
EndoS from Streptococcus pyogenes is hydrolyzed by the cysteine proteinase SpeB and requires glutamic acid 235 and tryptophans for IgG glycan-hydrolyzing activity., PMID:18182097
Endoglycosidase treatment abrogates IgG arthritogenicity: importance of IgG glycosylation in arthritis., PMID:17899548
Low antibody levels against cell wall-attached proteins of Streptococcus pyogenes predispose for severe invasive disease., PMID:14976595
EndoS and SpeB from Streptococcus pyogenes inhibit immunoglobulin-mediated opsonophagocytosis., PMID:12438337
Effect of SpeB and EndoS from Streptococcus pyogenes on human immunoglobulins., PMID:11598100
EndoS, a novel secreted protein from Streptococcus pyogenes with endoglycosidase activity on human IgG., PMID:11406581
For research use only. Not for human or drug use.
