Catalog No.
YXX22801
Expression system
E. coli
Species
Streptococcus pyogenes
Protein length
Ser81-Thr249
Predicted molecular weight
20.56 kDa
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Accession
WP_161710504.1
Applications
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol.
Reconstitution
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternative Names
sortase, sortase
Structure-Based Development of a Covalent Inhibitor Targeting Streptococcus Pyogenes over Staphylococcus Aureus Sortase A., PMID:40072260
LiaR-dependent gene expression contributes to antimicrobial responses in group A Streptococcus., PMID:39535201
A unique binding mode of P1' Leu-containing target sequences for Streptococcus pyogenes sortase A results in alternative cleavage., PMID:38179192
Structure-Guided Design, Synthesis, and Antivirulence Assessment of Covalent Staphylococcus aureus Sortase A Inhibitors., PMID:38170998
Structures of Streptococcus pyogenes class A sortase in complex with substrate and product mimics provide key details of target recognition., PMID:36055407
Identification and prioritization of promising lead molecules from Syzygium aromaticum against Sortase C from Streptococcus pyogenes: an in silico investigation., PMID:35706070
Evolution of Streptococcus pyogenes has maximized the efficiency of the Sortase A cleavage motif for cell wall transpeptidation., PMID:35430253
Covalent sortase A inhibitor ML346 prevents Staphylococcus aureus infection of Galleria mellonella., PMID:35308030
Structural and biochemical analyses of selectivity determinants in chimeric Streptococcus Class A sortase enzymes., PMID:34939250
RecT Recombinase Expression Enables Efficient Gene Editing in Enterococcus spp., PMID:34232061
Designed Streptococcus pyogenes Sortase A Accepts Branched Amines as Nucleophiles in Sortagging., PMID:33052658
Engineering the specificity of Streptococcus pyogenes sortase A by loop grafting., PMID:32501594
Development of an Enzyme-Mediated, Site-Specific Method to Conjugate Toll-Like Receptor 2 Agonists onto Protein Antigens: Toward a Broadly Protective, Four Component, Group A Streptococcal Self-Adjuvanting Lipoprotein-Fusion Combination Vaccine., PMID:32407620
The M Protein of Streptococcus pyogenes Strain AP53 Retains Cell Surface Functional Plasminogen Binding after Inactivation of the Sortase A Gene., PMID:32123038
Identification of potential antivirulence agents by substitution-oriented screening for inhibitors of Streptococcus pyogenes sortase A., PMID:30343193
Identification of sortase substrates by specificity profiling., PMID:28684010
A Novel "Prebinding" Strategy Dramatically Enhances Sortase-Mediated Coupling of Proteins to Liposomes., PMID:28358190
Variable region in streptococcal M-proteins provides stable binding with host fibrinogen for plasminogen-mediated bacterial invasion., PMID:28280245
Spatial Organization of Cell Wall-Anchored Proteins at the Surface of Gram-Positive Bacteria., PMID:27025379
Streptococcus pyogenes Sortase Mutants Are Highly Susceptible to Killing by Host Factors Due to Aberrant Envelope Physiology., PMID:26484774
Molecular determinants of bacterial sensitivity and resistance to mammalian Group IIA phospholipase A2., PMID:26079797
Site-specific immobilization of protein layers on gold surfaces via orthogonal sortases., PMID:25773291
Sortase A induces Th17-mediated and antibody-independent immunity to heterologous serotypes of group A streptococci., PMID:25232948
Antiinfective therapy with a small molecule inhibitor of Staphylococcus aureus sortase., PMID:25197057
Structure and activity of Streptococcus pyogenes SipA: a signal peptidase-like protein essential for pilus polymerisation., PMID:24911348
Intramolecular isopeptide but not internal thioester bonds confer proteolytic and significant thermal stability to the S. pyogenes pilus adhesin Spy0125., PMID:24123467
Site-specific N-terminal labeling of proteins using sortase-mediated reactions., PMID:23989674
SecA localization and SecA-dependent secretion occurs at new division septa in group B Streptococcus., PMID:23762438
An arm-swapped dimer of the Streptococcus pyogenes pilin specific assembly factor SipA., PMID:23747392
Intracellular protein cyclization catalyzed by exogenously transduced Streptococcus pyogenes sortase A., PMID:23587499
Cellular aspects of the distinct M protein and SfbI anchoring pathways in Streptococcus pyogenes., PMID:22512736
Protein ligation in living cells using sortase., PMID:22348280
Involvement of T6 pili in biofilm formation by serotype M6 Streptococcus pyogenes., PMID:22155780
A novel bacterial resistance mechanism against human group IIA-secreted phospholipase A2: role of Streptococcus pyogenes sortase A., PMID:22075700
Dynamic distribution of the SecA and SecY translocase subunits and septal localization of the HtrA surface chaperone/protease during Streptococcus pneumoniae D39 cell division., PMID:21990615
Structural differences between the Streptococcus agalactiae housekeeping and pilus-specific sortases: SrtA and SrtC1., PMID:21912586
Assembly mechanism of FCT region type 1 pili in serotype M6 Streptococcus pyogenes., PMID:21880740
Sortase-catalyzed transformations that improve the properties of cytokines., PMID:21297034
Crystal structure of Spy0129, a Streptococcus pyogenes class B sortase involved in pilus assembly., PMID:21264317
Cellular interactions by LPxTG-anchored pneumococcal adhesins and their streptococcal homologues., PMID:21199258
Autocatalytic intramolecular isopeptide bond formation in gram-positive bacterial pili: a QM/MM simulation., PMID:21142157
Roles of minor pilin subunits Spy0125 and Spy0130 in the serotype M1 Streptococcus pyogenes strain SF370., PMID:20639332
Crystal structure of the minor pilin FctB reveals determinants of Group A streptococcal pilus anchoring., PMID:20427291
The Corynebacterium diphtheriae shaft pilin SpaA is built of tandem Ig-like modules with stabilizing isopeptide and disulfide bonds., PMID:19805181
The interaction of the antimicrobial peptide cLEAP-2 and the bacterial membrane., PMID:19615343
Site-specific N- and C-terminal labeling of a single polypeptide using sortases of different specificity., PMID:19610623
Intramolecular isopeptide bonds give thermodynamic and proteolytic stability to the major pilin protein of Streptococcus pyogenes., PMID:19497855
For research use only. Not for human or drug use.
