Catalog No.
YHB32001
Expression system
E. coli
Species
Homo sapiens (Human)
Protein length
Leu108-Lys222
Predicted molecular weight
15.50 kDa
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Accession
O75822
Applications
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol.
Reconstitution
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternative Names
Eukaryotic translation initiation factor 3 subunit J, eIF3j, Eukaryotic translation initiation factor 3 subunit 1, eIF-3-alpha, eIF3 p35, EIF3J, EIF3S1, PRO0391
Yeast Eukaryotic Initiation Factor 4B Remodels the MRNA Entry Site on the Small Ribosomal Subunit., PMID:39847343
Stem-loop and circle-loop TADs generated by directional pairing of boundary elements have distinct physical and regulatory properties., PMID:39110491
The novel β-TrCP protein isoform hidden in circular RNA confers trastuzumab resistance in HER2-positive breast cancer., PMID:37783059
Rare genetic variants impact muscle strength., PMID:37301943
eIF3j inhibits translation of a subset of circular RNAs in eukaryotic cells., PMID:36330957
Rebirth of the translational machinery: The importance of recycling ribosomes., PMID:35147231
Differential proteomic analysis demonstrates follicle fluid participate immune reaction and protein translation in yak., PMID:35031034
eIF3k Domain-Containing Protein Regulates Conidiogenesis, Appressorium Turgor, Virulence, Stress Tolerance, and Physiological and Pathogenic Development of Magnaporthe oryzae Oryzae., PMID:34733303
eIF3j facilitates loading of release factors into the ribosome., PMID:34591963
A structural inventory of native ribosomal ABCE1-43S pre-initiation complexes., PMID:33289941
LncRNA EIF3J-AS1 enhanced esophageal cancer invasion via regulating AKT1 expression through sponging miR-373-3p., PMID:32811869
Transcriptome profiling and pathway analysis in squamous cell carcinoma of buccal mucosa., PMID:31930966
H3K27 acetylation-induced lncRNA EIF3J-AS1 improved proliferation and impeded apoptosis of colorectal cancer through miR-3163/YAP1 axis., PMID:31709617
Ribosomal Protein L13 Promotes IRES-Driven Translation of Foot-and-Mouth Disease Virus in a Helicase DDX3-Dependent Manner., PMID:31619563
Hypoxia-induced lncRNA EIF3J-AS1 accelerates hepatocellular carcinoma progression via targeting miR-122-5p/CTNND2 axis., PMID:31421822
Tetrapeptide 60-63 of human ribosomal protein uS3 is crucial for translation initiation., PMID:31356988
Hcr1/eIF3j Is a 60S Ribosomal Subunit Recycling Accessory Factor In Vivo., PMID:31269449
New liver cancer biomarkers: PI3K/AKT/mTOR pathway members and eukaryotic translation initiation factors., PMID:28715695
A helicase-independent activity of eIF4A in promoting mRNA recruitment to the human ribosome., PMID:28559306
Exploring accessibility of structural elements of the mammalian 40S ribosomal mRNA entry channel at various steps of translation initiation., PMID:27346718
Protein kinase CK2 potentiates translation efficiency by phosphorylating eIF3j at Ser127., PMID:25887626
Structure of a yeast 40S-eIF1-eIF1A-eIF3-eIF3j initiation complex., PMID:25664723
The translation initiation complex eIF3 in trypanosomatids and other pathogenic excavates--identification of conserved and divergent features based on orthologue analysis., PMID:25539953
Human eukaryotic initiation factor 2 (eIF2)-GTP-Met-tRNAi ternary complex and eIF3 stabilize the 43 S preinitiation complex., PMID:25246524
Functional and biochemical characterization of human eukaryotic translation initiation factor 3 in living cells., PMID:24912683
Human-like eukaryotic translation initiation factor 3 from Neurospora crassa., PMID:24250809
Spectrin domain of eukaryotic initiation factor 3a is the docking site for formation of the a:b:i:g subcomplex., PMID:23921387
Crystal structure of the RNA recognition motif of yeast translation initiation factor eIF3b reveals differences to human eIF3b., PMID:20862284
The C-terminal region of eukaryotic translation initiation factor 3a (eIF3a) promotes mRNA recruitment, scanning, and, together with eIF3j and the eIF3b RNA recognition motif, selection of AUG start codons., PMID:20584985
The indispensable N-terminal half of eIF3j/HCR1 cooperates with its structurally conserved binding partner eIF3b/PRT1-RRM and with eIF1A in stringent AUG selection., PMID:20060839
Human initiation factor eIF3 subunit b interacts with HCV IRES RNA through its N-terminal RNA recognition motif., PMID:19059401
Recycling of eukaryotic posttermination ribosomal complexes., PMID:17956730
eIF3j is located in the decoding center of the human 40S ribosomal subunit., PMID:17588516
The fission yeast ortholog of eIF3a subunit is not functional in Saccharomyces cerevisiae., PMID:17455792
Structure of eIF3b RNA recognition motif and its interaction with eIF3j: structural insights into the recruitment of eIF3b to the 40 S ribosomal subunit., PMID:17190833
Interaction of the RNP1 motif in PRT1 with HCR1 promotes 40S binding of eukaryotic initiation factor 3 in yeast., PMID:16581774
Changes in ribosomal binding activity of eIF3 correlate with increased translation rates during activation of T lymphocytes., PMID:15946946
Binding of eukaryotic initiation factor 3 to ribosomal 40S subunits and its role in ribosomal dissociation and anti-association., PMID:15703437
The j-subunit of human translation initiation factor eIF3 is required for the stable binding of eIF3 and its subcomplexes to 40 S ribosomal subunits in vitro., PMID:14688252
Characterization of eIF3k: a newly discovered subunit of mammalian translation initiation factor elF3., PMID:14519125
Dual function of eIF3j/Hcr1p in processing 20 S pre-rRNA and translation initiation., PMID:11560931
For research use only. Not for human or drug use.
