Catalog No.
YHA40301
Expression system
E. coli
Species
Homo sapiens (Human)
Protein length
Met1-His153
Predicted molecular weight
19.15 kDa
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Accession
O15067
Applications
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol.
Reconstitution
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternative Names
Phosphoribosylformylglycinamidine synthase, FGAM synthase, FGAMS, 6.3.5.3, Formylglycinamide ribonucleotide amidotransferase, FGAR amidotransferase, FGAR-AT, Formylglycinamide ribotide amidotransferase, Phosphoribosylformylglycineamide amidotransferase, PFAS, KIAA0361
Integrative in silico analysis of per- and polyfluorinated alkyl substances (PFAS)-associated molecular alterations in human cancers: a multi-cancer framework for predicting toxicogenomic disruption., PMID:40474796
Phosphoribosylformylglycinamidine Synthase (PFAS) Deficiency: Clinical, Genetic and Metabolic Characterisation of a Novel Defect in Purine de Novo Synthesis., PMID:40421664
Viral pseudo-enzyme facilitates KSHV lytic replication via suppressing PFAS-mediated RTA deamidation., PMID:40228741
Molecular docking-QSAR-Kronecker-regularized least squares-based multiple machine learning for assessment and prediction of PFAS-protein binding interactions., PMID:40179788
High-throughput screening of protein interactions with per- and polyfluoroalkyl substances (PFAS) used in photolithography., PMID:39837032
A high throughput assay for phosphoribosylformylglycinamidine synthase., PMID:39824442
NSUN2-mediated m5 C RNA methylation dictates retinoblastoma progression through promoting PFAS mRNA stability and expression., PMID:37228185
Genome-wide CRISPR screening reveals nucleotide synthesis negatively regulates autophagy., PMID:34000301
Dominant entropic binding of perfluoroalkyl substances (PFASs) to albumin protein revealed by 19F NMR., PMID:33297081
ERK2 Phosphorylates PFAS to Mediate Posttranslational Control of De Novo Purine Synthesis., PMID:32485148
Antiviral activity of a purine synthesis enzyme reveals a key role of deamidation in regulating protein nuclear import., PMID:31633017
Perfluorooctane Sulfonate (PFOS) Produces Dopaminergic Neuropathology in Caenorhabditis elegans., PMID:31428778
Protein interactome of the deamidase phosphoribosylformylglycinamidine synthetase (PFAS) by LC-MS/MS., PMID:30987822
Detecting Purinosome Metabolon Formation with Fluorescence Microscopy., PMID:29605921
N-Terminomics TAILS Identifies Host Cell Substrates of Poliovirus and Coxsackievirus B3 3C Proteinases That Modulate Virus Infection., PMID:29437971
Expression of the purine biosynthetic enzyme phosphoribosyl formylglycinamidine synthase in neurons., PMID:29337348
Gammaherpesviral Tegument Proteins, PML-Nuclear Bodies and the Ubiquitin-Proteasome System., PMID:29065450
Purine Biosynthesis Enzymes in Hippocampal Neurons., PMID:28866774
A missense mutation in PFAS (phosphoribosylformylglycinamidine synthase) is likely causal for embryonic lethality associated with the MH1 haplotype in Montbéliarde dairy cattle., PMID:28803020
Crystal structures of a subunit of the formylglycinamide ribonucleotide amidotransferase, PurS, from Thermus thermophilus, Sulfolobus tokodaii and Methanocaldococcus jannaschii., PMID:27487927
γHV68 vGAT: a viral pseudoenzyme pimping for PAMPs., PMID:25839430
Viral pseudo-enzymes activate RIG-I via deamidation to evade cytokine production., PMID:25752576
Quantitative analysis of purine nucleotides indicates that purinosomes increase de novo purine biosynthesis., PMID:25605736
Determination of the formylglycinamide ribonucleotide amidotransferase ammonia pathway by combining 3D-RISM theory with experiment., PMID:25551173
Murine gammaherpesvirus 68 ORF75c contains ubiquitin E3 ligase activity and requires PML SUMOylation but not other known cellular PML regulators, CK2 and E6AP, to mediate PML degradation., PMID:23541081
Formylglycinamide ribonucleotide amidotransferase from Salmonella typhimurium: role of ATP complexation and the glutaminase domain in catalytic coupling., PMID:22683785
Structure of N-formylglycinamide ribonucleotide amidotransferase II (PurL) from Thermus thermophilus HB8., PMID:22232163
Purine biosynthesis in archaea: variations on a theme., PMID:22168471
A link between impaired purine nucleotide synthesis and apoptosis in Drosophila melanogaster., PMID:21441212
Microtubule-assisted mechanism for functional metabolic macromolecular complex formation., PMID:20615962
Formylglycinamide ribonucleotide amidotransferase from Thermotoga maritima: structural insights into complex formation., PMID:18597481
Reversible compartmentalization of de novo purine biosynthetic complexes in living cells., PMID:18388293
Complexed structures of formylglycinamide ribonucleotide amidotransferase from Thermotoga maritima describe a novel ATP binding protein superfamily., PMID:17154526
Extending top-down mass spectrometry to proteins with masses greater than 200 kilodaltons., PMID:17023655
Crystal structure of phosphoribosylformyl-glycinamidine synthase II, PurS subunit (TM1244) from Thermotoga maritima at 1.90 A resolution., PMID:16865708
Crystal structure of phosphoribosylformylglycinamidine synthase II (smPurL) from Thermotoga maritima at 2.15 A resolution., PMID:16544324
Symbiotic abilities of Sinorhizobium fredii with modified expression of purL., PMID:16228203
Mapping of the bovine genes of the de novo AMP synthesis pathway., PMID:15566465
A model for the Bacillus subtilis formylglycinamide ribonucleotide amidotransferase multiprotein complex., PMID:15301532
Domain organization of Salmonella typhimurium formylglycinamide ribonucleotide amidotransferase revealed by X-ray crystallography., PMID:15301531
The formylglycinamide ribonucleotide amidotransferase complex from Bacillus subtilis: metabolite-mediated complex formation., PMID:15301530
Soybean FGAM synthase promoters direct ectopic nematode feeding site activity., PMID:15060594
[FGAM synthetase]., PMID:12629688
Crystal structure of MTH169, a crucial component of phosphoribosylformylglycinamidine synthetase., PMID:12211007
Linkage between toxin production and purine biosynthesis in Clostridium difficile., PMID:11800470
[Genetic control of purine biosynthesis in Pichia methanolica yeast. The ADE5 (PUR4) gene, controlling 5'-phosphoribosylformyl glycineamide synthetase]., PMID:11190471
The yexA gene product is required for phosphoribosylformylglycinamidine synthetase activity in Bacillus subtilis., PMID:10784038
Human phosphoribosylformylglycineamide amidotransferase (FGARAT): regional mapping, complete coding sequence, isolation of a functional genomic clone, and DNA sequence analysis., PMID:10548741
The human GARS-AIRS-GART gene encodes two proteins which are differentially expressed during human brain development and temporally overexpressed in cerebellum of individuals with Down syndrome., PMID:9328467
ADE6 gene of Saccharomyces cerevisiae yeast encoding formylglycinamidine-ribonucleotide synthetase. Cloning, sequencing, and analysis., PMID:9331966
For research use only. Not for human or drug use.
