Catalog No.
YXX54101
Expression system
E. coli
Species
Baker's yeast
Protein length
Met1-Arg613
Predicted molecular weight
68.76 kDa
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Accession
P11484
Applications
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol.
Reconstitution
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternative Names
Ribosome-associated molecular chaperone SSB1, 3.6.4.10, Cold-inducible protein YG101, Heat shock protein SSB1, Hsp70 chaperone Ssb, SSB1, YG101, YDL229W
Native Fold Delay and its implications for co-translational chaperone binding and protein aggregation., PMID:39955309
Role of J-domain Proteins in Yeast Physiology and Protein Quality Control., PMID:38331212
Zuo1, a ribosome-associated J protein, is involved in glucose repression in Saccharomyces cerevisiae., PMID:37550218
Yeast chaperones and ubiquitin ligases contribute to proteostasis during arsenite stress by preventing or clearing protein aggregates., PMID:37191881
Anti-Prion Systems in Saccharomyces cerevisiae Turn an Avalanche of Prions into a Flurry., PMID:36146752
Structural remodeling of ribosome associated Hsp40-Hsp70 chaperones during co-translational folding., PMID:35701497
Pathway of Hsp70 interactions at the ribosome., PMID:34580293
Normal levels of ribosome-associated chaperones cure two groups of [PSI+] prion variants., PMID:33020283
How Do Yeast Cells Contend with Prions?, PMID:32635197
A Drug Repurposing and Protein-Protein Interaction Network Study of Ribosomopathies Using Yeast as a Model System., PMID:31895625
Activity of the yeast cytoplasmic Hsp70 nucleotide-exchange factor Fes1 is regulated by reversible methionine oxidation., PMID:31806703
Cotranslational assembly of protein complexes in eukaryotes revealed by ribosome profiling., PMID:30158700
Yeast Prions Compared to Functional Prions and Amyloids., PMID:29698650
Anti-Prion Systems in Yeast and Inositol Polyphosphates., PMID:29377675
The Hsp70 homolog Ssb affects ribosome biogenesis via the TORC1-Sch9 signaling pathway., PMID:29038496
Two chaperones locked in an embrace: structure and function of the ribosome-associated complex RAC., PMID:28771464
Profiling Ssb-Nascent Chain Interactions Reveals Principles of Hsp70-Assisted Folding., PMID:28708998
Two alternative binding mechanisms connect the protein translocation Sec71-Sec72 complex with heat shock proteins., PMID:28286332
Multivalent contacts of the Hsp70 Ssb contribute to its architecture on ribosomes and nascent chain interaction., PMID:27917864
Prion-Associated Toxicity is Rescued by Elimination of Cotranslational Chaperones., PMID:27828954
The Hsp70 homolog Ssb and the 14-3-3 protein Bmh1 jointly regulate transcription of glucose repressed genes in Saccharomyces cerevisiae., PMID:27001512
Improvement of lactic acid production in Saccharomyces cerevisiae by a deletion of ssb1., PMID:26660479
Quantitative proteomics and network analysis of SSA1 and SSB1 deletion mutants reveals robustness of chaperone HSP70 network in Saccharomyces cerevisiae., PMID:25689132
Release factor eRF3 mediates premature translation termination on polylysine-stalled ribosomes in Saccharomyces cerevisiae., PMID:25154418
The cotranslational function of ribosome-associated Hsp70 in eukaryotic protein homeostasis., PMID:23332755
Ribosome-associated complex and Ssb are required for translational repression induced by polylysine segments within nascent chains., PMID:23007158
Structural analysis of the interactions between hsp70 chaperones and the yeast DNA replication protein Orc4p., PMID:20732327
The ribosome-bound Hsp70 homolog Ssb of Saccharomyces cerevisiae., PMID:20226819
Selection to maintain paralogous amino acid differences under the pressure of gene conversion in the heat-shock protein genes in yeast., PMID:19745001
The Hsp70 homolog Ssb is essential for glucose sensing via the SNF1 kinase network., PMID:19723765
Yeast Uri1p promotes translation initiation and may provide a link to cotranslational quality control., PMID:19387492
Hsp104, Hsp70 and Hsp40 interplay regulates formation, growth and elimination of Sup35 prions., PMID:18833196
Alleviation of deleterious effects of protein mutation through inactivation of molecular chaperones., PMID:18762987
Variant-specific [PSI+] infection is transmitted by Sup35 polymers within [PSI+] aggregates with heterogeneous protein composition., PMID:18353968
Hsp110 chaperones regulate prion formation and propagation in S. cerevisiae by two discrete activities., PMID:18335038
Functional characterization of the atypical Hsp70 subunit of yeast ribosome-associated complex., PMID:17901048
Fine-tuning of translation termination efficiency in Saccharomyces cerevisiae involves two factors in close proximity to the exit tunnel of the ribosome., PMID:17483428
The specialized cytosolic J-protein, Jjj1, functions in 60S ribosomal subunit biogenesis., PMID:17242366
Association of protein biogenesis factors at the yeast ribosomal tunnel exit is affected by the translational status and nascent polypeptide sequence., PMID:17229726
Identification of the divergent calmodulin binding motif in yeast Ssb1/Hsp75 protein and in other HSP70 family members., PMID:17146552
Fes1p acts as a nucleotide exchange factor for the ribosome-associated molecular chaperone Ssb1p., PMID:17132105
Why molecular chaperones buffer mutational damage: a case study with a yeast Hsp40/70 system., PMID:16849597
Sbp1p affects translational repression and decapping in Saccharomyces cerevisiae., PMID:16782896
Specific effects of ribosome-tethered molecular chaperones on programmed -1 ribosomal frameshifting., PMID:16607023
Systems analyses reveal two chaperone networks with distinct functions in eukaryotic cells., PMID:16413483
The yeast Hsp110 Sse1 functionally interacts with the Hsp70 chaperones Ssa and Ssb., PMID:16221677
The chaperones MPP11 and Hsp70L1 form the mammalian ribosome-associated complex., PMID:16002468
The Hsp70 Ssz1 modulates the function of the ribosome-associated J-protein Zuo1., PMID:15908962
Identification of genes and proteins induced during the lag and early exponential phase of lager brewing yeasts., PMID:15659180
Broad sensitivity of Saccharomyces cerevisiae lacking ribosome-associated chaperone ssb or zuo1 to cations, including aminoglycosides., PMID:15643063
For research use only. Not for human or drug use.
