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Recombinant E. coli O157:H7 FliC/Flagellin Protein, N-His

Catalog #:   YXX07801 Specific References (50) DATASHEET
Applications: ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Expression system: E. coli
Accession: Q7AD06
Protein length: Gly179-Gly491
Overview

Catalog No.

YXX07801

Expression system

E. coli

Species

Escherichia coli

Protein length

Gly179-Gly491

Predicted molecular weight

33.59 kDa

Nature

Recombinant

Endotoxin level

Please contact with the lab for this information.

Purity

>90% as determined by SDS-PAGE.

Accession

Q7AD06

Applications

ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress

Form

Lyophilized

Storage buffer

Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol.

Reconstitution

Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.

Shipping

In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Alternative Names

Flagellin, fliC, ECs_2662

Data Image
References

Isolation of an E. coli flagellotrophic Jumbophage SHEFM2K that replicates in cytoplasmic putative assembly areas., PMID:39908945

Detection of Escherichia coli O157H7 and Campylobacter jejuni in Bovine Carcasses in Two Slaughterhouses in Bio-Bío District, Chile., PMID:38568114

Surface proteins of Shiga toxin-producing Escherichia coli mediate association with milk fat globules in raw milk., PMID:37426002

Adhesion mechanism and biofilm formation of Escherichia coli O157:H7 in infected cucumber (Cucumis sativus L.)., PMID:35473968

A Novel Small RNA Promotes Motility and Virulence of Enterohemorrhagic Escherichia coli O157:H7 in Response to Ammonium., PMID:33688013

Evaluation and comparison of recombinase polymerase amplification coupled with lateral-flow bioassay for Escherichia coli O157:H7 detection using diifeerent genes., PMID:33479307

Mechanisms involved in the adaptation of Escherichia coli O157:H7 to the host intestinal microenvironment., PMID:33346356

Occurrence, virulence genes, and antimicrobial profiles of Escherichia coli O157 isolated from ruminants slaughtered in Al Ain, United Arab Emirates., PMID:32677884

PchE Regulation of Escherichia coli O157:H7 Flagella, Controlling the Transition to Host Cell Attachment., PMID:32605187

Simultaneous detection of Salmonella spp., Pseudomonas aeruginosa, Bacillus cereus, and Escherichia coli O157:H7 in environmental water using PMA combined with mPCR., PMID:32583285

Bifidobacterium mongoliense genome seems particularly adapted to milk oligosaccharide digestion leading to production of antivirulent metabolites., PMID:32380943

A fluorescence quenching based gene assay for Escherichia coli O157:H7 using graphene quantum dots and gold nanoparticles., PMID:31745737

Molecular Prediction of the O157:H-Negative Phenotype Prevalent in Australian Shiga Toxin-Producing Escherichia coli Cases Improves Concordance of In Silico Serotyping with Phenotypic Motility., PMID:29367299

Prevalence, quantification and isolation of pathogenic shiga toxin Escherichia coli O157:H7 along the production and supply chain of pork around Hubei Province of China., PMID:29273508

Lysogenisation of Shiga toxin-encoding bacteriophage represses cell motility., PMID:29225287

Characteristics of Shiga Toxin-Producing Escherichia coli O157 in Slaughtered Reindeer from Northern Finland., PMID:28207302

Differential modulation of flagella expression in enterohaemorrhagic Escherichia coli O157: H7 by intestinal short-chain fatty acid mixes., PMID:27535670

Inhibitory effects of grape seed extract on growth, quorum sensing, and virulence factors of CDC "top-six" non-O157 Shiga toxin producing E. coli., PMID:27099982

Molecular Characterization of Human Atypical Sorbitol-Fermenting Enteropathogenic Escherichia coli O157 Reveals High Diversity., PMID:26984976

The effect of oxidative stress on gene expression of Shiga toxin-producing Escherichia coli (STEC) O157:H7 and non-O157 serotypes., PMID:26318408

The impact of oregano (Origanum heracleoticum) essential oil and carvacrol on virulence gene transcription by Escherichia coli O157:H7., PMID:25790499

Expression of stress and virulence genes in Escherichia coli O157:H7 heat shocked in fresh dairy compost., PMID:25581175

Escherichia coli strains expressing H12 antigens demonstrate an increased ability to attach to abiotic surfaces as compared with E. coli strains expressing H7 antigens., PMID:24880987

A novel small regulatory RNA enhances cell motility in enterohemorrhagic Escherichia coli., PMID:24646762

Escherichia coli O157:H7 lacking the qseBC-encoded quorum-sensing system outcompetes the parental strain in colonization of cattle intestines., PMID:24413602

An O island 172 encoded RNA helicase regulates the motility of Escherichia coli O157:H7., PMID:23785398

Hha controls Escherichia coli O157:H7 biofilm formation by differential regulation of global transcriptional regulators FlhDC and CsgD., PMID:23377937

Shiga toxin-producing Escherichia coli in beef retail markets from Argentina., PMID:23346554

Proteomic and phenotypic analysis of triclosan tolerant verocytotoxigenic Escherichia coli O157:H19., PMID:23313217

Non-O157:H7 Shiga toxin-producing Escherichia coli in bovine rectums and surface water streams on a beef cattle farm in Argentina., PMID:22994915

Bovine milk fat globule membrane affects virulence expression in Escherichia coli O157:H7., PMID:22981580

Molecular characterization reveals three distinct clonal groups among clinical shiga toxin-producing Escherichia coli strains of serogroup O103., PMID:22718945

Role of lipid rafts and flagellin in invasion of colonic epithelial cells by Shiga-toxigenic Escherichia coli O113:H21., PMID:22689816

Serotypes, virulence genes, and intimin types of shiga toxin-producing Escherichia coli and enteropathogenic Escherichia coli isolated from mastitic milk relevant to human health in Egypt., PMID:22229945

Phosphorylation of the epidermal growth factor receptor (EGFR) is essential for interleukin-8 release from intestinal epithelial cells in response to challenge with Escherichia coli O157 : H7 flagellin., PMID:21546588

Detection of Escherichia coli O157:H7 virulence genes in isolates from beef, pork, water, human and animal species in the northwest province, South Africa: public health implications., PMID:21272634

Detection of Shiga toxin-producing Escherichia coli serotypes O26:H11, O103:H2, O111:H8, O145:H28, and O157:H7 in raw-milk cheeses by using multiplex real-time PCR., PMID:21239543

Simplex and multiplex real-time PCR assays for the detection of flagellar (H-antigen) fliC alleles and intimin (eae) variants associated with enterohaemorrhagic Escherichia coli (EHEC) serotypes O26:H11, O103:H2, O111:H8, O145:H28 and O157:H7., PMID:20618885

A multiplex PCR procedure for the detection of six major virulence genes in Escherichia coli O157:H7., PMID:20472005

Evaluation of the effects of sdiA, a luxR homologue, on adherence and motility of Escherichia coli O157 : H7., PMID:20110300

Escherichia coli O157 in bovine feces and surface water streams in a beef cattle farm of Argentina., PMID:20092405

Prevalence of Escherichia coli O157:H7 in gut contents of beef cattle at slaughter., PMID:19899963

Low-density macroarray targeting non-locus of enterocyte effacement effectors (nle genes) and major virulence factors of Shiga toxin-producing Escherichia coli (STEC): a new approach for molecular risk assessment of STEC isolates., PMID:19880649

Performance comparison of a fliC(h7) real-time PCR assay with an H7 latex agglutination test for confirmation of the H type of Escherichia coli O157:H7., PMID:19833045

Interaction of Escherichia coli O157:H7 with leafy green produce., PMID:19681282

Escherichia coli O157:H7 strains that persist in feedlot cattle are genetically related and demonstrate an enhanced ability to adhere to intestinal epithelial cells., PMID:19617387

Escherichia coli O157:H7 stress and virulence gene expression on Romaine lettuce using comparative real-time PCR., PMID:19248811

Phenotypic and genotypic traits of Shiga toxin-negative E. coli O157:H7/H(-) bovine and porcine strains., PMID:19245340

Evaluation of the 'GeneDisc' real-time PCR system for detection of enterohaemorrhagic Escherichia coli (EHEC) O26, O103, O111, O145 and O157 strains according to their virulence markers and their O- and H-antigen-associated genes., PMID:19191965

An investigation of the expression and adhesin function of H7 flagella in the interaction of Escherichia coli O157 : H7 with bovine intestinal epithelium., PMID:19016776

Datasheet
$ 313
Product specifications
100 μg 313 1 mg 1629

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Recombinant E. coli O157:H7 FliC/Flagellin Protein, N-His [YXX07801]
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