Catalog No.
RGK26502
Host species
Mouse
Isotype
VHH-mFc
Clonality
Monoclonal
Target
ALFA-tag, (P)SRLEEELRRRLTE(P), SRLEEELRRRLTE, ALFA tag
Concentration
4.9 mg/ml
Endotoxin level
Please contact with the lab for this information.
Purity
>95% as determined by SDS-PAGE.
Purification
Protein A/G purified from cell culture supernatant.
Applications
ELISA, FCM, IHC, IP, WB
Form
Liquid
Storage buffer
100 mM Pro-Ac, 20 mM Arg, pH 5.0
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Clone ID
SAA2164
Modular cell line for scalable and rapid in vitro evaluation of chimeric antigen receptors., PMID:40374131
Penta-ALFA-Tagged Substrates for Self-Labelling Tags Allow Signal Enhancement in Microscopy., PMID:40222732
Multimerized epitope tags for high-sensitivity protein detection., PMID:40191928
A cautionary note on the potential pitfalls of using N-terminal truncated CD63 to label small extracellular vesicles., PMID:40025121
The potential of ALFA-tag and tyramide-based fluorescence signal amplification to expand the CRISPR-based DNA imaging toolkit., PMID:39106316
Boosting the toolbox for live imaging of translation., PMID:39060168
Versatile nanobody-based approach to image, track and reconstitute functional Neurexin-1 in vivo., PMID:39025931
Patient-Reported Outcomes From the Phase III HIMALAYA Study of Tremelimumab Plus Durvalumab in Unresectable Hepatocellular Carcinoma., PMID:38805668
Grafting the ALFA tag for structural studies of aquaporin Z., PMID:38361954
Application of nanotags and nanobodies for live cell single-molecule imaging of the Z-ring in Escherichia coli., PMID:37022498
ALFA-PRF: a novel approach to detect murine perforin release from CTLs into the immune synapse., PMID:36618385
Application of ALFA-Tagging in the Nematode Model Organisms Caenorhabditis elegans and Pristionchus pacificus., PMID:36497133
A Light-Activatable Photocaged Variant of the Ultra-High Affinity ALFA-Tag Nanobody., PMID:35411584
Development and Validation of Arc Nanobodies: New Tools for Probing Arc Dynamics and Function., PMID:35307777
Structural basis of an epitope tagging system derived from Haloarcula marismortui bacteriorhodopsin I D94N and its monoclonal antibody GD-26., PMID:34499806
Discovery and Characterization of an ALFA-Tag-Specific Affinity Resin Optimized for Protein Purification at Low Temperatures in Physiological Buffer., PMID:33673130
Serological Test to Determine Exposure to SARS-CoV-2: ELISA Based on the Receptor-Binding Domain of the Spike Protein (S-RBDN318-V510) Expressed in Escherichia coli., PMID:33578665
The ALFA-tag is a highly versatile tool for nanobody-based bioscience applications., PMID:31562305
Investigations on therapeutic glucocerebrosidases through paired detection with fluorescent activity-based probes., PMID:28207759
Systemic administration of recombinant interferon alfa in carcinoma patients upregulates the expression of the carcinoma-associated antigens tumor-associated glycoprotein-72 and carcinoembryonic antigen., PMID:8683233
For research use only. Not for human or drug use.
