Catalog No.
RWK30501
Species reactivity
Lama glama (Llama)
Host species
Mouse
Isotype
IgG2a
Clonality
Monoclonal
Applications
ELISA, FCM, IF, IHC, WB
Target
Alpaca, Nanobody, VHH, Secondary Antibody
Concentration
1.18 mg/ml
Endotoxin level
Please contact with the lab for this information.
Purity
>95% as determined by SDS-PAGE.
Purification
Protein A/G purified from cell culture supernatant.
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Clone ID
NabFab
Note
For research use only.
Domain swapping of a llama VHH domain builds a crystal-wide beta-sheet structure., PMID:15094039
Heating as a rapid purification method for recovering correctly-folded thermotolerant VH and VHH domains., PMID:17257422
An intrabody based on a llama single-domain antibody targeting the N-terminal alpha-helical multimerization domain of HIV-1 rev prevents viral production., PMID:20406803
Structures of a key interaction protein from the Trypanosoma brucei editosome in complex with single domain antibodies., PMID:20969962
Atomic structure of a nanobody-trapped domain-swapped dimer of an amyloidogenic beta2-microglobulin variant., PMID:21220305
Analysis and modeling of the variable region of camelid single-domain antibodies., PMID:21525384
Antibody engineering reveals the important role of J segments in the production efficiency of llama single-domain antibodies in Saccharomyces cerevisiae., PMID:22143875
Llama-derived single domain antibodies specific for Abrus agglutinin., PMID:22174977
Semiautomated panning of naive camelidae libraries and selection of single-domain antibodies against peptide antigens., PMID:22886248
Selection and evaluation of single domain antibodies toward MS2 phage and coat protein., PMID:22898187
Single domain antibody against carcinoembryonic antigen-related cell adhesion molecule 6 (CEACAM6) inhibits proliferation, migration, invasion and angiogenesis of pancreatic cancer cells., PMID:22918079
Structure of a low-melting-temperature anti-cholera toxin: llama V(H)H domain., PMID:23385744
Production, crystallization and X-ray diffraction analysis of two nanobodies against the Duffy binding-like (DBL) domain DBL6ℇ-FCR3 of the Plasmodium falciparum VAR2CSA protein., PMID:23519802
A stably expressed llama single-domain intrabody targeting Rev displays broad-spectrum anti-HIV activity., PMID:25453342
Can template-based protein models guide the design of sequence fitness for enhanced thermal stability of single domain antibodies?, PMID:26374895
Structural Effects of Two Camelid Nanobodies Directed to Distinct C-Terminal Epitopes on α-Synuclein., PMID:27096466
Structural analysis of nested neutralizing and non-neutralizing B cell epitopes on ricin toxin's enzymatic subunit., PMID:27159829
Trivalency of a Nanobody Specific for the Human Respiratory Syncytial Virus Fusion Glycoprotein Drastically Enhances Virus Neutralization and Impacts Escape Mutant Selection., PMID:27550346
Analysis of the binding loops configuration and surface adaptation of different crystallized single-domain antibodies in response to various antigens., PMID:27862476
A toolbox of anti-mouse and anti-rabbit IgG secondary nanobodies., PMID:29263082
Sandwich pair nanobodies, a potential tool for electrochemical immunosensing serum prostate-specific antigen with preferable specificity., PMID:29935325
CXCR4-targeting nanobodies differentially inhibit CXCR4 function and HIV entry., PMID:30342024
A class II MHC-targeted vaccine elicits immunity against SARS-CoV-2 and its variants., PMID:34654739
VHH Structural Modelling Approaches: A Critical Review., PMID:35409081
Structural basis for llama nanobody recognition and neutralization of HIV-1 at the CD4-binding site., PMID:35413243
A Novel Cost-Effective Nanobody against Fumonisin B1 Contaminations: Efficacy Test in Dairy Milk and Chickens., PMID:36548718
VHH CDR-H3 conformation is determined by VH germline usage., PMID:37598276
Evaluation of the Potential Impact of In Silico Humanization on VHH Dynamics., PMID:37834033
For research use only. Not for human or drug use.
