Catalog No.
YHB19401
Expression system
E. coli
Species
Homo sapiens (Human)
Protein length
Met1-Met894
Predicted molecular weight
112.20 kDa
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Accession
O75417
Applications
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol.
Reconstitution
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternative Names
POLH, DNA polymerase eta, DNA polymerase theta, POLQ
Anemarchalconyn, a natural alkyne ketone compound, inhibits HCC cell growth by suppressing Polθ and inducing synthetic lethality in Homologous recombination deficiency cells., PMID:40215812
FAN1-mediated translesion synthesis and POLQ/HELQ-mediated end joining generate interstrand crosslink-induced mutations., PMID:40082407
Harnessing transcriptional regulation of alternative end-joining to predict cancer treatment., PMID:40061566
The human HELQ helicase and XRN2 exoribonuclease cooperate in R-loop resolution., PMID:39965657
Synthetic lethality of mRNA quality control complexes in cancer., PMID:39910291
Integrated Analysis of Polymerase Family Gene Mutations in Acute Myeloid Leukemia: Clinical Features, Prognosis, and Bioinformatics Insights., PMID:39768855
The interplay of DNA repair context with target sequence predictably biases Cas9-generated mutations., PMID:39592573
Elucidating acquired PARP inhibitor resistance in advanced prostate cancer., PMID:39577422
POLQ knockdown inhibits proliferation, migration, and invasion by inducing cell cycle arrest in colorectal cancer., PMID:39520627
The Proteogenomics of Prostate Cancer Radioresistance., PMID:39166898
Polθ: emerging synthetic lethal partner in homologous recombination-deficient tumors., PMID:39122831
Analysis of translesion polymerases in colorectal cancer cells following cetuximab treatment: A network perspective., PMID:39102671
Current status and prospect of the DNA double-strand break repair pathway in colorectal cancer development and treatment., PMID:39059591
Induction of the DNA-Repair Gene POLQ only in BRCA1-mutant Breast-Cancer Cells by Methionine Restriction., PMID:38944428
Targeting S6K/NFκB/SQSTM1/Polθ signaling to suppress radiation resistance in prostate cancer., PMID:38925361
Single-Stranded DNA Gap Accumulation Is a Functional Biomarker for USP1 Inhibitor Sensitivity., PMID:38885312
Computational identification of mitochondrial dysfunction biomarkers in severe SARS-CoV-2 infection: Facilitating therapeutic applications of phytomedicine., PMID:38878325
Dynamic stem-loop extension by Pol θ and templated insertion during DNA repair., PMID:38876299
Exosomal circPOLQ promotes macrophage M2 polarization via activating IL-10/STAT3 axis in a colorectal cancer model., PMID:38782541
Cellular landscape of adrenocortical carcinoma at single-nuclei resolution., PMID:38759836
Iron promotes ovarian cancer malignancy and advances platinum resistance by enhancing DNA repair via FTH1/FTL/POLQ/RAD51 axis., PMID:38740757
HELQ deficiency impairs the induction of primordial germ cell-like cells., PMID:38720471
Modulation of the microhomology-mediated end joining pathway suppresses large deletions and enhances homology-directed repair following CRISPR-Cas9-induced DNA breaks., PMID:38685010
Human DNA polymerase θ does not harbor intrinsic nuclease activity., PMID:38640889
Incidence and molecular characteristics of deficient mismatch repair conditions across nine different tumors and identification of germline variants involved in Lynch-like syndrome., PMID:38615286
POLQ inhibition attenuates the stemness and ferroptosis resistance in gastric cancer cells via downregulation of dihydroorotate dehydrogenase., PMID:38575587
POLQ identifies a better response subset to immunotherapy in muscle-invasive bladder cancer with high PD-L1., PMID:38457207
The m6A demethylase FTO targets POLQ to promote ccRCC cell proliferation and genome stability maintenance., PMID:38270643
Deficiency of both classical and alternative end-joining pathways leads to a synergistic defect in double-strand break repair but not to an increase in homology-dependent gene targeting in Arabidopsis., PMID:38179887
Genetic separation of Brca1 functions reveal mutation-dependent Polθ vulnerabilities., PMID:38001070
Impact of Germline Depletion of Bonus on Chromatin State in Drosophila Ovaries., PMID:37998364
HELQ as a DNA helicase: Its novel role in normal cell function and tumorigenesis (Review)., PMID:37921071
Transformation and regeneration of DNA polymerase Θ mutant rice plants., PMID:37681196
CSB Regulates Pathway Choice in Response to DNA Replication Stress Induced by Camptothecin., PMID:37569794
Dynamic viral integration patterns actively participate in the progression of BK polyomavirus-associated diseases after renal transplantation., PMID:37507072
DPPA5A suppresses the mutagenic TLS and MMEJ pathways by modulating the cryptic splicing of Rev1 and Polq in mouse embryonic stem cells., PMID:37459543
RHINO directs MMEJ to repair DNA breaks in mitosis., PMID:37440612
The interplay of DNA repair context with target sequence predictably biases Cas9-generated mutations., PMID:37425722
Simultaneous Targeting of DNA Polymerase Theta and PARP1 or RAD52 Triggers Dual Synthetic Lethality in Homologous Recombination-Deficient Leukemia Cells., PMID:37358557
Diagnostic rate of germline pathogenic variants in pancreatic ductal adenocarcinoma patients using whole genome sequencing., PMID:37234870
Genomic profiling of a metastatic anaplastic melanocytic neuroectodermal tumor arising from a mature thymic teratoma as part of a mediastinal germ cell tumor., PMID:37160315
[Study on enhanced sensitivity to DNA damage in POLQ knocking-down salivary of adenoid cystic carcinoma-83 cells]., PMID:37153990
Identification of POLQ as a key gene in cervical cancer progression using integrated bioinformatics analysis and experimental validation., PMID:37114556
POLQ inhibition elicits an immune response in homologous recombination-deficient pancreatic adenocarcinoma via cGAS/STING signaling., PMID:36976649
Rare germline variants in pancreatic cancer and multiple primary cancers: an autopsy study., PMID:36896836
Identification of Critical Molecular Factors and Side Effects Underlying the Response to Thalicthuberine in Prostate Cancer: A Systems Biology Approach., PMID:36789117
Characterization of DNA Polymerase Genes in Amazonian Amerindian Populations., PMID:36672794
Targeting polymerase θ impairs tumorigenesis and enhances radiosensitivity in lung adenocarcinoma., PMID:36642785
Mutations of TP53 and genes related to homologous recombination repair in breast cancer with germline BRCA1/2 mutations., PMID:36604691
DNA polymerase θ protects leukemia cells from metabolically induced DNA damage., PMID:36580665
For research use only. Not for human or drug use.
