Catalog No.
YHN30601
Expression system
E. coli
Species
Homo sapiens (Human)
Protein length
Gln14-Lys126
Predicted molecular weight
15.16 kDa
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Accession
Q96EP1
Applications
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol.
Reconstitution
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternative Names
E3 ubiquitin-protein ligase CHFR, RNF196, Checkpoint with forkhead and RING finger domains protein, CHFR, RING-type E3 ubiquitin transferase CHFR, RING finger protein 196
NUDT16 enhances the resistance of cancer cells to DNA-damaging agents by regulating replication fork stability via reversing HMGA1 ADP-ribosylation., PMID:40288645
DNMT1 is required for efficient DSB repair and maintenance of replication fork stability, and its loss reverses resistance to PARP inhibitors in cancer cells., PMID:40234721
Circulating Tumor DNA Methylation Is a Biomarker of Poor Recurrence-free Survival in Locally-Advanced Rectal Cancer., PMID:40155013
Deep learning prioritizes cancer mutations that alter protein nucleocytoplasmic shuttling to drive tumorigenesis., PMID:40087285
Prognostic utility of circulating tumor DNA methylation analysis in stage IV colorectal cancer., PMID:39155651
The dePARylase NUDT16 promotes radiation resistance of cancer cells by blocking SETD3 for degradation via reversing its ADP-ribosylation., PMID:38272222
Ubiquitin ligase CHFR mediated degradation of VE-cadherin through ubiquitylation disrupts endothelial adherens junctions., PMID:37852964
CHFR promotes metastasis of human gastric carcinoma by activating AKT and ERK via NRF2- ROS axis., PMID:37024798
WHO grading system for invasive pulmonary lung adenocarcinoma reveals distinct molecular signature: An analysis from the cancer genome atlas database., PMID:35339455
Organoid screening reveals epigenetic vulnerabilities in human colorectal cancer., PMID:35273398
CHFR-Promoter-Methylation Status Is Predictive of Response to Irinotecan-based Systemic Chemotherapy in Advanced Colorectal Cancer., PMID:35093868
CHFR and Paclitaxel Sensitivity of Ovarian Cancer., PMID:34885153
Circ-CHFR modulates the proliferation, migration, and invasion of ox-LDL-induced human aorta vascular smooth muscle cells through the miR-214-3p/PAPPA axis., PMID:34842180
Effect of MAP3K8 on Prognosis and Tumor-Related Inflammation in Renal Clear Cell Carcinoma., PMID:34567061
CHFR regulates chemoresistance in triple-negative breast cancer through destabilizing ZEB1., PMID:34462429
CHFR-mediated degradation of RNF126 confers sensitivity to PARP inhibitors in triple-negative breast cancer cells., PMID:34388456
Cytosolic GDH1 degradation restricts protein synthesis to sustain tumor cell survival following amino acid deprivation., PMID:34269483
Vaccinia-related kinase 2 drives pancreatic cancer progression by protecting Plk1 from Chfr-mediated degradation., PMID:34140642
Circ_CHFR Promotes Platelet-Derived Growth Factor-BB-Induced Proliferation, Invasion, and Migration in Vascular Smooth Muscle Cells via the miR-149-5p/NRP2 Axis., PMID:33990513
CHFR‑mediated epithelial‑to‑mesenchymal transition promotes metastasis in human breast cancer cells., PMID:33880594
A phase 2 trial of gemcitabine and docetaxel in patients with metastatic colorectal adenocarcinoma with methylated checkpoint with forkhead and ring finger domain promoter and/or microsatellite instability phenotype., PMID:33811727
Kaposi's sarcoma-associated herpesvirus processivity factor (PF-8) recruits cellular E3 ubiquitin ligase CHFR to promote PARP1 degradation and lytic replication., PMID:33508027
Human papillomavirus-positivity is associated with EREG down-regulation and promoter hypermethylation in head and neck squamous cell carcinoma., PMID:33007298
Oncogenic Potential of Bisphenol A and Common Environmental Contaminants in Human Mammary Epithelial Cells., PMID:32466334
Methylation patterns in dysplasia in inflammatory bowel disease patients., PMID:32456486
Nutlin-3a suppresses poly (ADP-ribose) polymerase 1 by mechanisms different from conventional PARP1 suppressors in a human breast cancer cell line., PMID:32405340
Circ_CHFR expedites cell growth, migration and inflammation in ox-LDL-treated human vascular smooth muscle cells via the miR-214-3p/Wnt3/β-catenin pathway., PMID:32271446
Identification of Subtypes of Barrett's Esophagus and Esophageal Adenocarcinoma Based on DNA Methylation Profiles and Integration of Transcriptome and Genome Data., PMID:32032585
Case Study: Landscape of Chimeric RNAs in Bladder Cancer., PMID:31728975
MORC2 regulates DNA damage response through a PARP1-dependent pathway., PMID:31616951
Poly(ADP‑ribose) polymerase 1/2 inhibitors decrease the ubiquitination of ALC1 mediated by CHFR in breast cancer., PMID:31322269
Antileukemia Effects of Notch-Mediated Inhibition of Oncogenic PLK1 in B-Cell Acute Lymphoblastic Leukemia., PMID:31227645
DNA methylation and hydroxymethylation patterns in acute myeloid leukemia patients with mutations in DNMT3A and IDH1/2 and their combinations., PMID:30988238
Promoter hypermethylation in ductal carcinoma in situ of the male breast., PMID:30921768
CHFR promotes the migration of human gastric cancer cells by inducing epithelial-to-mesenchymal transition in a HDAC1-dependent manner., PMID:30799937
Spatiotemporal regulation of the Dma1-mediated mitotic checkpoint coordinates mitosis with cytokinesis., PMID:30600396
Association of CHFR Promoter Methylation with Treatment Outcomes of Irinotecan-Based Chemotherapy in Metastatic Colorectal Cancer., PMID:30562637
Stabilization of HDAC1 via TCL1-pAKT-CHFR axis is a key element for NANOG-mediated multi-resistance and stem-like phenotype in immune-edited tumor cells., PMID:30060952
Drosophila bendless catalyzes K63-linked polyubiquitination and is involved in the response to DNA damage., PMID:29518634
The inhibition of UBC13 expression and blockage of the DNMT1-CHFR-Aurora A pathway contribute to paclitaxel resistance in ovarian cancer., PMID:29367628
Upregulation of the checkpoint protein CHFR is associated with tumor suppression in pancreatic cancers., PMID:29344247
Analysis of DNA methylation and microRNA expression in NUT (nuclear protein in testis) midline carcinoma of the sinonasal tract: a clinicopathological, immunohistochemical and molecular genetic study., PMID:29322795
Platform-independent gene expression signature differentiates sessile serrated adenomas/polyps and hyperplastic polyps of the colon., PMID:29284484
Methylation-independent CHFR expression is a potential biomarker affecting prognosis in acute myeloid leukemia., PMID:29115660
Phase II study of nab-paclitaxel in refractory small bowel adenocarcinoma and CpG island methylator phenotype (CIMP)-high colorectal cancer., PMID:29069279
Sustained expression of miR-26a promotes chromosomal instability and tumorigenesis through regulation of CHFR., PMID:28126920
SMARCB1/INI1-deficient sinonasal carcinoma shows methylation of RASSF1 gene: A clinicopathological, immunohistochemical and molecular genetic study of a recently described entity., PMID:28069272
CHFR negatively regulates SIRT1 activity upon oxidative stress., PMID:27883020
Arsenic trioxide induces cell cycle arrest and alters DNA methylation patterns of cell cycle regulatory genes in colorectal cancer cells., PMID:27769816
Evaluation of the acceptability of improved supplementary foods for the treatment of moderate acute malnutrition in Burkina Faso using a mixed method approach., PMID:26752599
For research use only. Not for human or drug use.
