Catalog No.
MHK15202
Species reactivity
Human
Host species
Mouse
Isotype
IgG2a
Clonality
Monoclonal
Immunogen
Synthesized peptide (Cys-GEEEGE) conjugated with KLH.
Target
Tubulin B-alpha-1, TUBA3, TUBA1A, Tubulin alpha-3 chain, Alpha-tubulin 3, Tubulin alpha-1A chain,Detyrosinated alpha tubulin
Concentration
0.97 mg/ml
Endotoxin level
Please contact with the lab for this information.
Purity
>95% as determined by SDS-PAGE.
Purification
Protein A/G purified from cell culture supernatant.
Accession
Q71U36
Applications
ELISA, IHC, WB
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Clone ID
1A047
TUBA4A downregulation as observed in ALS post-mortem motor cortex causes ALS-related abnormalities in zebrafish., PMID:38463699
Microtubule detyrosination by VASH1/SVBP is regulated by the conformational state of tubulin in the lattice., PMID:37716348
Expression and localization of tubulin isotypes and its mRNAs during Thecaphora frezii developments., PMID:36208160
Detyrosinated α-Tubulin, Vimentin and PD-L1 in Circulating Tumor Cells (CTCs) Isolated from Non-Small Cell Lung Cancer (NSCLC) Patients., PMID:35207643
Pseudophosphatase MK-STYX Alters Histone Deacetylase 6 Cytoplasmic Localization, Decreases Its Phosphorylation, and Increases Detyrosination of Tubulin., PMID:30909412
Evaluation of α-tubulin, detyrosinated α-tubulin, and vimentin in CTCs: identification of the interaction between CTCs and blood cells through cytoskeletal elements., PMID:29976237
Reduced Adrenomedullin Parallels Microtubule Dismantlement in Frontotemporal Lobar Degeneration., PMID:29671277
Post-translational incorporation of 3,4-dihydroxyphenylalanine into the C terminus of α-tubulin in living cells., PMID:29341414
Combination Treatment with Orlistat-Containing Nanoparticles and Taxanes Is Synergistic and Enhances Microtubule Stability in Taxane-Resistant Prostate Cancer Cells., PMID:28615298
Fixation methods can differentially affect ciliary protein immunolabeling., PMID:28352462
Post-Translational Incorporation of L-Phenylalanine into the C-Terminus of α-Tubulin as a Possible Cause of Neuronal Dysfunction., PMID:27905536
Loukoumasomes Are Distinct Subcellular Structures from Rods and Rings and Are Structurally Associated with MAP2 and the Nuclear Envelope in Retinal Cells., PMID:27798680
Evidence for new C-terminally truncated variants of α- and β-tubulins., PMID:26739754
Sulphamoylated 2-methoxyestradiol analogues induce apoptosis in adenocarcinoma cell lines., PMID:24039728
Investigating tubulin posttranslational modifications with specific antibodies., PMID:23973077
Tubulin posttranslational modifications induced by cadmium in the sponge Clathrina clathrus., PMID:23765032
Anti-migratory effect of vinflunine in endothelial and glioblastoma cells is associated with changes in EB1 C-terminal detyrosinated/tyrosinated status., PMID:23750272
Specific in vivo labeling of tyrosinated α-tubulin and measurement of microtubule dynamics using a GFP tagged, cytoplasmically expressed recombinant antibody., PMID:23555790
The ability to induce microtubule acetylation is a general feature of formin proteins., PMID:23110170
Cap-Gly proteins at microtubule plus ends: is EB1 detyrosination involved?, PMID:22432029
Differential distribution of posttranslationally modified microtubules in osteoclasts., PMID:21421796
Post-translational modifications to Toxoplasma gondii alpha- and beta-tubulins include novel C-terminal methylation., PMID:19886702
Lack of stabilized microtubules as a result of the absence of major maps in CAD cells does not preclude neurite formation., PMID:19878302
Rab2 utilizes glyceraldehyde-3-phosphate dehydrogenase and protein kinase C{iota} to associate with microtubules and to recruit dynein., PMID:19106097
The Aspergillus nidulans kinesin-3 UncA motor moves vesicles along a subpopulation of microtubules., PMID:19037104
Evidence for microtubule target engagement in tumors of patients receiving ixabepilone., PMID:18094432
Preparation and characterization of posttranslationally modified tubulins from Artemia franciscana., PMID:18085221
Miniaturization and validation of a sensitive multiparametric cell-based assay for the concomitant detection of microtubule-destabilizing and microtubule-stabilizing agents., PMID:16751334
Distinct localization of a beta-tubulin epitope in the Tetrahymena thermophila and Paramecium caudatum cortex., PMID:16228897
Monocilia on chicken embryonic endocardium in low shear stress areas., PMID:16145662
Localization and loss-of-function implicates ciliary proteins in early, cytoplasmic roles in left-right asymmetry., PMID:16059906
Low expression of human tubulin tyrosine ligase and suppressed tubulin tyrosination/detyrosination cycle are associated with impaired neuronal differentiation in neuroblastomas with poor prognosis., PMID:15382060
Odontoblast primary cilia: facts and hypotheses., PMID:14984754
Post-translational incorporation of the antiproliferative agent azatyrosine into the C-terminus of alpha-tubulin., PMID:12852782
Incorporation of 3-nitrotyrosine into the C-terminus of alpha-tubulin is reversible and not detrimental to dividing cells., PMID:12383263
Microtubule cold stability in supporting cells of the gerbil auditory sensory epithelium: correlation with tubulin post-translational modifications., PMID:11810314
Export from pericentriolar endocytic recycling compartment to cell surface depends on stable, detyrosinated (glu) microtubules and kinesin., PMID:11809825
The differential distribution of acetylated and detyrosinated alpha-tubulin in the microtubular cytoskeleton and primary cilia of hyaline cartilage chondrocytes., PMID:11693300
Length control of primary cilia: analysis of monociliate and multiciliate PtK1 cells., PMID:11374436
Distribution of polyglutamylated tubulin in the flagellar apparatus of green flagellates., PMID:11056523
Incorporation of nitrotyrosine into alpha-tubulin by recombinant mammalian tubulin-tyrosine ligase., PMID:11004583
Posttranslationally modified tubulins and microtubule organization in hemocytes of the brine shrimp, Artemia franciscana., PMID:10814999
Beating rate of isolated neonatal cardiomyocytes is regulated by the stable microtubule subset., PMID:10775146
Tubulin carboxypeptidase/microtubules association can be detected in the distal region of neural processes., PMID:10685601
Tubulin-tyrosine ligase, a long-lasting enigma., PMID:10685598
Composition and organization of tubulin isoforms reveals a variety of axonemal models., PMID:10668099
An Assessment of alphaalpha-Tubulin Isotype Modification in Developing Cotton Fiber., PMID:10648195
Glycogen synthase kinase 3beta phosphorylation of microtubule-associated protein 1B regulates the stability of microtubules in growth cones., PMID:10504342
Association of tubulin carboxypeptidase with microtubules in living cells., PMID:10191280
Monoclonal antibody ID5: epitope characterization and minimal requirements for the recognition of polyglutamylated alpha- and beta-tubulin., PMID:10082420
For research use only. Not for human or drug use.
