Sotrovimab ELISA kit (KDV00301)

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Applications：Used for the quantitative determination of Sotrovimab concentration in serum and plasma.

Sample type：Plasma, Serum

Range：156.25 ng/mL - 10,000 ng/mL

Overview

Description

PRINCIPLE OF THE ASSAY This assay employs the quantitative competitive enzyme immunoassay technique. Recombinant SARS-CoV-2 RBD has been pre-coated onto a microplate. Standards or samples are premixed with biotin-labeled antibody and then pipetted into the wells. Sotrovimab in the sample competitively binds to the pre-coated protein with biotin-labeled Sotrovimab. After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in inversely proportion to the amount of Sotrovimab bound in the initial step. The color development is stopped and the intensity of the color is measured.

Catalog No.

KDV00301

Applications

Used for the quantitative determination of Sotrovimab concentration in serum and plasma.

Stability and Storage

When the kit was stored at the recommended temperature for 6 months, the signal intensity decreased by less than 20%. For unopened kits, if you want to prolong the storage time, please store the Standard, Detection A, Detection B and Microplate at - 20 ℃, the rest reagents should be store at 4℃.

Detection method

Colorimetric

Precision

Intra-Assay Precision (Precision within an assay): <20%

Three samples of known concentration were tested sixteen times on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays): <20%

Three samples of known concentration were tested in twenty four separate assays to assess inter-assay precision.

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (ng/mL)	3831.47	1199.88	314.02	4124.27	1082.39	380.26
Standard deviation	268.61	52.47	44.62	618.41	204.23	75.62
CV (%)	7.01	4.37	14.21	14.99	18.87	19.89

Sample type

Plasma, Serum

Assay type

Quantitative

Range

156.25 ng/mL - 10,000 ng/mL

Recovery

80-120%

Alternative Names

VIR-7831,GSK4182136,S309,GSK-4182136,CAS: 2423014-07-5

Shipping

2-8 ℃

Note

For Research Use Only.

Data Image

Experiment Example

CALCULATION OF RESULTS
Average the duplicate readings for each standard and sample. Construct a standard curve by plotting the mean absorbance for each standard on the Y-axis against the concentration on the X-axis and draw a best fit curve through the points on the graph. The data may be linearized by plotting the log of the Sotrovimab concentrations versus the log of the O.D. and the best fit line can be determined by regression analysis. This procedure will produce an adequate but less precise fit of the data. If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor.

References

Datasheet

Document Download

Sotrovimab ELISA kit.pdf

$ 1128

Product specifications

96T 1128

Contact Information

Order: order@antibodysystem.com

Mail: support@antibodysystem.com

Distributor list

For research use only. Not for human or drug use.

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