SARS-CoV-2 Nucleocapsid Protein (NP) ELISA Kit

Catalog No.

KVV18001

Target

Nucleocapsid, NP

Applications

Used for the quantitative determination of SARS-CoV-2 Nucleocapsid Protein (NP) concentration in bronchoalveolar lavage fluid and nasopharyngeal swab samples.

Detection method

Colorimetric

Sample type

Serum, Plasma and Cell culture supernatant.

Assay type

Quantitative

Range

312.5 - 20,000 pg/mL

Sensitivity

167.31 pg/mL

Precision

Intra-Assay Precision (Precision within an assay): <10%

Three samples of known concentration were tested sixteen times on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays): <15%

Three samples of known concentration were tested in twenty four separate assays to assess inter-assay precision.

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/mL)	9633.1	2956.4	1027.6	10199.5	2764.3	988.2
Standard deviation	573.1	118.3	58.3	599.1	139.3	84.6
CV (%)	5.9	4.0	5.7	5.9	5.0	8.6

 

Recovery

80-120%

Shipping

2-8 ℃

Stability and Storage

When the kit was stored at the recommended temperature for 6 months, the signal intensity decreased by less than 20%. For unopened kits, if you want to prolong the storage time, please store the Standard, Detection A, Detection B and Microplate at - 20℃, the rest reagents should be store at 4℃.

Dynamic ensembles of SARS-CoV-2 N-protein reveal head-to-head coiled-coil-driven oligomerization and phase separation., PMID:40503686

Single-particle quantification of SARS-CoV-2 virus-like particles using flow virometry., PMID:40483334

Seroprevalence Trends of Antibodies to SARS-CoV-2 in South Korea, 2021-2022: A Repeated Cross-Sectional Study., PMID:40462400

SARS-CoV-2-specific humoral immunity in a Norwegian cohort between 2020 and 2023., PMID:40462062

A Nanomaterial-Independent Biosensor Based on Gallium Arsenide High-Electron-Mobility Transistors for Rapid and Ultra-Sensitive Pathogen Detection., PMID:40461437

SARS-CoV-2 N protein exerts antitumor effects in NSCLC by inducing DNA damage and augmenting chemotherapeutic sensitivity., PMID:40451965

Evidence of SARS-CoV-2 bacteriophage potential in human gut microbiota., PMID:40444030

The Overlooked Nucleocapsid Response: A Cohort Study of SARS-CoV-2 Vaccines in Brazil., PMID:40430765

Screening bacterial effectors and human virus proteins in yeast to identify host factors driving tombusvirus RNA recombination: a role for autophagy and membrane phospholipid content., PMID:40422074

Intrinsic Factors Behind Long COVID: VI. Combined Impact of G3BPs and SARS-CoV-2 Nucleocapsid Protein on the Viral Persistence and Long COVID., PMID:40415285

SERS-based lateral flow immunoassay for rapid and sensitive sensing of nucleocapsid protein toward SARS-CoV-2 screening in clinical samples., PMID:40409906

Phosphorylation Changes SARS-CoV-2 Nucleocapsid Protein's Structural Dynamics and Its Interaction With RNA., PMID:40375582

Natural product sennoside B disrupts liquid-liquid phase separation of SARS-CoV-2 nucleocapsid protein by inhibiting its RNA-binding activity., PMID:40371698

Development of monoclonal antibodies against SARS-CoV-2 nucleocapsid protein for COVID-19 antigen detection., PMID:40361217

Transfer of SARS-CoV-2 nucleocapsid protein to uninfected epithelial cells induces antibody-mediated complement deposition., PMID:40343796

SARS-CoV-2 N protein interacts with SLC7A11 to cause ferroptosis in acute lung injury., PMID:40342111

Plasma SARS-CoV-2 nucleocapsid antigen levels are associated with lung infection and tissue-damage biomarkers., PMID:40339608

High-resolution structure reveals enhanced 14-3-3 binding by a mutant SARS-CoV-2 nucleoprotein variant with improved replicative fitness., PMID:40318379

Ultra-sensitive heterojunction double gate BioTFET device for SARS-CoV-2 biomolecules detection., PMID:40307462

Variant mutation G215C in SARS-CoV-2 nucleocapsid enhances viral infection via altered genomic encapsidation., PMID:40299982

Identification and Characterization of a Novel Protein-Protein Interaction Among SARS-CoV-2 Nucleocapsid, Host SFPQ, and hnRNP U and Its Potential Role in Virus Replication., PMID:40259455

SARS-CoV-2 nucleocapsid protein induces a Mincle-dependent macrophage inflammatory response in acute kidney injury., PMID:40244324

Construction and immunogenicity analysis of a recombinant baculovirus targeting the N protein of SARS-CoV-2., PMID:40237823

Optimization of A549 Cell Transfection Efficiency with a Plasmid Encoding the N-Protein of the SARS-CoV-2 Virus., PMID:40216710

Targeting USP22 to promote K63-linked ubiquitination and degradation of SARS-CoV-2 nucleocapsid protein., PMID:40183543

Detection of IgG Antibodies Against COVID-19 N-Protein by Hybrid Graphene-Nanorod Sensor., PMID:40136961

Nucleocapsid-directed antibody testing is unsuitable to estimate hybrid immunity against SARS-CoV-2, a longitudinal cross-border study in the Meuse-Rhine Euroregion., PMID:40132351

Increase of VEGF and Fibronectin expression and ultrastructural alterations of intercellular junctions in a swab negative patient after SARS-COV-2 infection., PMID:40114185

Structural insights into the RNA binding inhibitors of the C-terminal domain of the SARS-CoV-2 nucleocapsid., PMID:40113149

Time course and determinants of the antibody response to SARS-CoV-2 in Costa Rica: the RESPIRA study., PMID:40102764

Chemokines simultaneously bind SARS-CoV-2 nucleocapsid protein RNA-binding and dimerization domains., PMID:40097964

Targeting the liquid-liquid phase separation of nucleocapsid broadly inhibits the replication of SARS-CoV-2 strains., PMID:40086356

Characterization of the binding features between SARS-CoV-2 5'-proximal transcripts of genomic RNA and nucleocapsid proteins., PMID:40077853

Nucleocapsid Protein of SARS-CoV-2 Upregulates RANTES Expression in A172 Glioblastoma Cells., PMID:40076291

Ultrasensitive, Real-Time Detection of Viral Antigens and RNA Enabled by Scalable Graphene-Based FET Sensors for Pathogen Detection: A Case Study on COVID-19., PMID:40073430

Vaccination with Plasmids Encoding the Fusion Proteins D-S1, D-S1N and O-SN from SARS-CoV-2 Induces an Effective Humoral and Cellular Immune Response in Mice., PMID:40006682

Immunoassay Detection of SARS-CoV-2 Using Monoclonal Antibody Binding to Viral Nucleocapsid Protein., PMID:39989430

Development of a Peptide Aptamer-Based TRFIA for the Quantitive Detection of SARS-CoV-2 Nucleocapsid Protein., PMID:39985615

"All-U-Want" Strand Displacement Amplification: A Versatile Signal Amplification Method for Nucleic Acid Biosensing., PMID:39951690

Characterising the SARS-CoV-2 nucleocapsid (N) protein antibody response., PMID:39922387

Revealing the Potential of a Chimaera: a Peptide-Peptide Nucleic Acid Molecule Designed To Interact with the SARS-CoV-2 Nucleocapsid Protein., PMID:39912211

Comparative evaluation of cell-based assay technologies for scoring drug-induced condensation of SARS-CoV-2 nucleocapsid protein., PMID:39894078

Effectiveness of Frequent Point-of-Care Molecular COVID-19 Surveillance in a Rural Workplace: Nonrandomized Controlled Clinical Trial Among Miners., PMID:39869851

An electrochemical microsensor of the SARS-CoV-2 nucleocapsid protein based on a surface-imprinted acupuncture needle., PMID:39865998

Structural insights into nucleocapsid protein variability: Implications for PJ34 efficacy against SARS-CoV-2., PMID:39848104

The R203M and D377Y mutations of the nucleocapsid protein promote SARS-CoV-2 infectivity by impairing RIG-I-mediated antiviral signaling., PMID:39841800

SARS-CoV-2 Is an Electricity-Driven Virus., PMID:39838019

Retraction of: Structural Insights of the SARS-CoV-2 Nucleocapsid Protein: Implications for the Inner-workings of Rapid Antigen Tests., PMID:39837351

The Blood-Cerebrospinal Fluid Barrier as a Potential Entry Site for the SARS-CoV-2 Virus., PMID:39835622

Plasmon-Enhanced Fluorescence Paper Lateral Flow Strip for Point-of-Care Testing of SARS-CoV-2 Antigens., PMID:39834249

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