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SARS-CoV-2 Nucleocapsid Protein (NP) ELISA Kit (KVV18001)

Applications:Used for the quantitative determination of SARS-CoV-2 Nucleocapsid Protein (NP) concentration in bronchoalveolar lavage fluid and nasopharyngeal swab samples.
Sample type:Serum, Plasma and Cell culture supernatant.
Sensitivity:167.3 pg/mL
Range:312.5 - 20,000 pg/mL
Overview

Catalog No.

KVV18001

Target

Nucleocapsid, NP

Applications

Used for the quantitative determination of SARS-CoV-2 Nucleocapsid Protein (NP) concentration in bronchoalveolar lavage fluid and nasopharyngeal swab samples.

Detection method

Colorimetric

Sample type

Serum, Plasma and Cell culture supernatant.

Assay type

Quantitative

Range

312.5 - 20,000 pg/mL

Sensitivity

167.3 pg/mL

Precision

Intra-Assay Precision (Precision within an assay): <10%

Three samples of known concentration were tested sixteen times on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays): <15%

Three samples of known concentration were tested in twenty four separate assays to assess inter-assay precision.

 

Intra-Assay Precision

Inter-Assay Precision

Sample

1

2

3

1

2

3

n

16

16

16

24

24

24

Mean (pg/mL)

9633.1

2956.4

1027.6

10199.5

2764.3

988.2

Standard deviation

573.1

118.3

58.3

599.1

139.3

84.6

CV (%)

5.9

4.0

5.7

5.9

5.0

8.6

 

Recovery

80-120%

Shipping

2-8 ℃

Stability and Storage

When the kit was stored at the recommended temperature for 6 months, the signal intensity decreased by less than 20%. For unopened kits, if you want to prolong the storage time, please store the Standard, Detection A, Detection B and Microplate at - 20℃, the rest reagents should be store at 4℃.

Data Image
  • Experiment Example
    CALCULATION OF RESULTS
    Average the duplicate readings for each standard and sample. Construct a standard curve by plotting the mean absorbance for each standard on the Y-axis against the concentration on the X-axis and draw a best fit curve through the points on the graph. The data may be linearized by plotting the log of the SARS-CoV-2 Nucleocapsid Protein (NP) concentrations versus the log of the O.D. and the best fit line can be determined by regression analysis. This procedure will produce an adequate but less precise fit of the data. If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor.
References
Datasheet
$ 548
Product specifications
96T 548

Contact Information

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Distributor list

For research use only. Not for human or drug use.

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SARS-CoV-2 Nucleocapsid Protein (NP) ELISA Kit [KVV18001]
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