Catalog No.
KAV00305
Description
This assay employs the quantitative indirect enzyme immunoassay technique. Recombinant SARS-CoV-2 RBD has been pre-coated onto a microplate. Standards or samples are pipetted into the wells and any Human Anti-SARS-CoV-2 RBD IgM present is bound by the immobilized protein. After washing away any unbound substances, a biotin-labeled Mouse Anti-Human IgM antibody is added to the wells. After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Human Anti-SARS-CoV-2 RBD IgM bound in the initial step. The color development is stopped and the intensity of the color is measured.
Target
RBD
Applications
Used for the quantitative determination of Anti-SARS-CoV-2 RBD Human IgM concentration in serum and plasma.
Detection method
Colorimetric
Sample type
Plasma, Serum
Assay type
Quantitative
Range
31.25 - 2,000 ng/mL
Sensitivity
3.6 ng/mL
Precision
Intra-Assay Precision (Precision within an assay): <10%
Three samples of known concentration were tested sixteen times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): <15%
Three samples of known concentration were tested in twenty four separate assays to assess inter-assay precision.
|
|
Intra-Assay Precision |
Inter-Assay Precision |
||||
|
Sample |
1 |
2 |
3 |
1 |
2 |
3 |
|
n |
16 |
16 |
16 |
24 |
24 |
24 |
|
Mean (ng/mL) |
1180.71 |
293.52 |
71.77 |
1017.39 |
251.72 |
65.66 |
|
Standard deviation |
40.78 |
5.62 |
3.12 |
72.02 |
11.95 |
2.86 |
|
CV (%) |
3.45 |
1.91 |
4.35 |
7.08 |
4.75 |
4.36 |
Recovery
80-120%
Shipping
2-8 ℃
Stability and Storage
When the kit was stored at the recommended temperature for 6 months, the signal intensity decreased by less than 20%. For unopened kits, if you want to prolong the storage time, please store the Standard, Detection A, Detection B and Microplate at - 20℃, the rest reagents should be store at 4℃.
For research use only. Not for human or drug use.
