Catalog No.
KAV00304
Description
This assay employs the quantitative indirect enzyme immunoassay technique. Recombinant SARS-CoV-2 RBD has been pre-coated onto a microplate. Standards or samples are pipetted into the wells and any Human Anti-SARS-CoV-2 RBD IgG present is bound by the immobilized protein. After washing away any unbound substances, a biotin-labeled Mouse Anti-Human IgG antibody is added to the wells. After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Human Anti-SARS-CoV-2 RBD IgG bound in the initial step. The color development is stopped and the intensity of the color is measured.
Target
RBD
Applications
Used for the quantitative determination of Anti-SARS-CoV-2 RBD Human IgG concentration in serum and plasma.
Detection method
Colorimetric
Sample type
Plasma, Serum
Assay type
Quantitative
Range
125 - 8,000 pg/mL
Sensitivity
52.31 pg/mL
Precision
Intra-Assay Precision (Precision within an assay): <10%
Three samples of known concentration were tested sixteen times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): <15%
Three samples of known concentration were tested in twenty four separate assays to assess inter-assay precision.
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|
Intra-Assay Precision
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Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
16
|
16
|
16
|
24
|
24
|
24
|
|
Mean (pg/mL)
|
3190.88
|
569.64
|
160.59
|
3521.55
|
669.60
|
166.70
|
|
Standard deviation
|
95.73
|
28.13
|
6.66
|
90.00
|
34.72
|
12.38
|
|
CV (%)
|
3.00
|
4.94
|
4.15
|
2.56
|
5.18
|
7.43
|
Recovery
80-120%
Shipping
2-8 ℃
Stability and Storage
When the kit was stored at the recommended temperature for 6 months, the signal intensity decreased by less than 20%. For unopened kits, if you want to prolong the storage time, please store the Standard, Detection A, Detection B and Microplate at - 20℃, the rest reagents should be store at 4℃.
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Efficient transplacental transfer of SARS-CoV-2 antibodies between naturally exposed mothers and infants in Accra, Ghana., PMID:38730052
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Efficacy of Sinopharm® COVID-19 Vaccine in Hemodialysis Patients: A Preliminary Report., PMID:35962641
Evaluation of Performance of Detection of Immunoglobulin G and Immunoglobulin M Antibody Against Spike Protein of SARS-CoV-2 by a Rapid Kit in a Real-Life Hospital Setting., PMID:35558113
Anti-SARS-CoV-2 IgG and Neutralizing Antibody Levels in Patients with Past COVID-19 Infection: A Longitudinal Study., PMID:35378574
Comparison of antibody response to SARS-CoV-2 after two doses of inactivated virus and BNT162b2 mRNA vaccines in kidney transplant., PMID:35198159
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Timeline of SARS-CoV-2 Spread in Italy: Results from an Independent Serological Retesting., PMID:35062265
Development and validation of novel kit for quantification of SARS-CoV-2 antibodies on clinical samples., PMID:34919976
Two novel SARS-CoV-2 surrogate virus neutralization assays are suitable for assessing successful immunization with mRNA-1273., PMID:34563583
Evaluation of a Commercial Culture-Free Neutralization Antibody Detection Kit for Severe Acute Respiratory Syndrome-Related Coronavirus-2 and Comparison With an Antireceptor-Binding Domain Enzyme-Linked Immunosorbent Assay., PMID:34136587
Robust and low-cost ELISA based on IgG-Fc tagged recombinant proteins to screen for anti-SARS-CoV-2 antibodies., PMID:34051226
COVID-19 in Russia: Clinical and Immunological Features of the First-Wave Patients., PMID:33959390
Application of newly developed SARS-CoV2 serology test along with real-time PCR for early detection in health care workers and on-time plasma donation., PMID:33869895
For research use only. Not for human or drug use.
