Catalog No.
PVV28801
Species reactivity
Porcine transmissible gastroenteritis coronavirus (strain Purdue) (TGEV)
Host species
Rabbit
Isotype
IgG
Clonality
Polyclonal
Immunogen
E. coli - derived recombinant TGEV N/Nucleoprotein (Met1-Asn382).
Applications
ELISA, IHC, IF, WB
Recommended Dilution
| Application |
Dilution |
| ELISA |
1:4000-1:8000, IHC: 1:50-1:100, IF: 1:50-1:100, WB: 1:1000-1:4000 |
Target
Nucleoprotein, Nucleocapsid protein, NC, Protein N, N, 6
Concentration
1.23 mg/ml
Purification
Purified by antigen affinity column.
Accession
P04134
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Note
For research use only.
Organization of two transmissible gastroenteritis coronavirus membrane protein topologies within the virion and core., PMID:11711614
Use of monoclonal antibodies in blocking ELISA detection of transmissible gastroenteritis virus in faeces of piglets., PMID:15876221
Transmissible gastroenteritis virus infection: a vanishing specter., PMID:16716052
Coronavirus nucleocapsid protein is an RNA chaperone., PMID:16979208
Complete genomic sequences, a key residue in the spike protein and deletions in nonstructural protein 3b of US strains of the virulent and attenuated coronaviruses, transmissible gastroenteritis virus and porcine respiratory coronavirus., PMID:17023013
Mutational analysis of aminopeptidase N, a receptor for several group 1 coronaviruses, identifies key determinants of viral host range., PMID:17093189
Porcine innate and adaptative immune responses to influenza and coronavirus infections., PMID:17135502
Cloning and expression of nucleocapsid protein gene of TGEV HB06 strain., PMID:32214985
An antibody derivative expressed from viral vectors passively immunizes pigs against transmissible gastroenteritis virus infection when supplied orally in crude plant extracts., PMID:17309733
Porcine aminopeptidase N is a functional receptor for the PEDV coronavirus., PMID:17467767
Two-way antigenic cross-reactivity between severe acute respiratory syndrome coronavirus (SARS-CoV) and group 1 animal CoVs is mediated through an antigenic site in the N-terminal region of the SARS-CoV nucleoprotein., PMID:17913799
Identification of sugar residues involved in the binding of TGEV to porcine brush border membranes., PMID:19057868
Retrospective serological survey on selected viral pathogens in wild boar populations in Germany., PMID:32214937
Intragastric administration of attenuated Salmonella typhimurium harbouring transmissible gastroenteritis virus (TGEV) DNA vaccine induced specific antibody production., PMID:19573642
Oral immunization of mice with recombinant Lactococcus lactis expressing porcine transmissible gastroenteritis virus spike glycoprotein., PMID:19629668
[Porcine aminopeptidase N is a functional receptor for the PEDV coronavirus]., PMID:19634766
Binding characterization of determinants in porcine aminopeptidase N, the cellular receptor for transmissible gastroenteritis virus., PMID:20643168
Production and characterization of a monoclonal antibody against spike protein of transmissible gastroenteritis virus., PMID:20715993
Characterization and utility of monoclonal antibodies against spike protein of transmissible gastroenteritis virus., PMID:21223341
Antigenic modules in the N-terminal S1 region of the transmissible gastroenteritis virus spike protein., PMID:21228126
Immunogenicity and protective efficacy in dogs of an MF59™-adjuvanted vaccine against recombinant canine/porcine coronavirus., PMID:21272607
Immunogenic characterization and epitope mapping of transmissible gastroenteritis virus RNA dependent RNA polymerase., PMID:21513742
Bacterial expression of antigenic sites A and D in the spike protein of transmissible gastroenteritis virus and evaluation of their inhibitory effects on viral infection., PMID:21701858
Generation of a mouse scFv library specific for porcine aminopeptidase N using the T7 phage display system., PMID:22481024
Structural bases of coronavirus attachment to host aminopeptidase N and its inhibition by neutralizing antibodies., PMID:22876187
A monoclonal antibody against transmissible gastroenteritis virus generated via immunization of a DNA plasmid bearing TGEV S1 gene., PMID:23600507
Antigenic relationships among porcine epidemic diarrhea virus and transmissible gastroenteritis virus strains., PMID:25589635
Phage display for identifying peptides that bind the spike protein of transmissible gastroenteritis virus and possess diagnostic potential., PMID:26013256
Development of an indirect ELISA, blocking ELISA, fluorescent microsphere immunoassay and fluorescent focus neutralization assay for serologic evaluation of exposure to North American strains of Porcine Epidemic Diarrhea Virus., PMID:26232106
Construction of an oral vaccine for transmissible gastroenteritis virus based on the TGEV N gene expressed in an attenuated Salmonella typhimurium vector., PMID:26297958
Characterization of Two Monoclonal Antibodies That Recognize Linker Region and Carboxyl Terminal Domain of Coronavirus Nucleocapsid Protein., PMID:27689694
The Identification and Characterization of Two Novel Epitopes on the Nucleocapsid Protein of the Porcine Epidemic Diarrhea Virus., PMID:27991537
Reactivity of Porcine Epidemic Diarrhea Virus Structural Proteins to Antibodies against Porcine Enteric Coronaviruses: Diagnostic Implications., PMID:28202790
Contribution of porcine aminopeptidase N to porcine deltacoronavirus infection., PMID:29636467
Two critical N-terminal epitopes of the nucleocapsid protein contribute to the cross-reactivity between porcine epidemic diarrhea virus and porcine transmissible gastroenteritis virus., PMID:30652967
Evaluation of the Serologic Cross-Reactivity between Transmissible Gastroenteritis Coronavirus and Porcine Respiratory Coronavirus Using Commercial Blocking Enzyme-Linked Immunosorbent Assay Kits., PMID:30867325
Recombinant Chimeric Transmissible Gastroenteritis Virus (TGEV) - Porcine Epidemic Diarrhea Virus (PEDV) Virus Provides Protection against Virulent PEDV., PMID:31349683
Identification of a Novel Linear B-Cell Epitope on the Nucleocapsid Protein of Porcine Deltacoronavirus., PMID:31963776
Identification and characterization of linear B cell epitopes on the nucleocapsid protein of porcine epidemic diarrhea virus using monoclonal antibodies., PMID:32142743
Agrodiag PorCoV: A multiplex immunoassay for the differential diagnosis of porcine enteric coronaviruses., PMID:32562689
Assessments of different inactivating reagents in formulating transmissible gastroenteritis virus vaccine., PMID:33097081
Generation and functional analysis of single chain variable fragments (scFvs) targeting the nucleocapsid protein of Porcine epidemic diarrhea virus., PMID:35024918
Serological survey suggests circulation of coronavirus on wild Suina from Argentina, 2014-2017., PMID:35652967
Identification of a novel linear B-cell epitope in porcine deltacoronavirus nucleocapsid protein., PMID:36602561
Development of a novel double-antibody sandwich quantitative ELISA for detecting SADS-CoV infection., PMID:36809389
A Customized Novel Blocking ELISA for Detection of Bat-Origin Swine Acute Diarrhea Syndrome Coronavirus Infection., PMID:37272819
A novel linear B cell epitope of the canine coronavirus nucleocapsid protein identified by a monoclonal antibody., PMID:38677126
Development and application of a blocking ELISA based on a N protein monoclonal antibody for the antibody detection against porcine reproductive and respiratory syndrome virus 2., PMID:38679249
Cleavage of the selective autophagy receptor NBR1 by the PDCoV main protease NSP5 impairs autophagic degradation of the viral envelope protein., PMID:40047225
Establishment of a pseudovirus neutralization assay for TGEV., PMID:40276514
For research use only. Not for human or drug use.
