Catalog No.
KAH02251
Description
PRINCIPLE OF THE ASSAY This assay employs the quantitative competitive enzyme immunoassay technique. Pembrolizumab has been pre-coated onto a microplate. Standards or samples are premixed with biotin-labeled Human CD279 antigen and then pipetted into the wells. Anti-Pembrolizumab Neutralizing Antibody in the sample competitively binds to Pembrolizumab with the biotin-labeled Human CD279 antigen. After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in inversely proportion to the amount of Anti-Pembrolizumab Neutralizing Antibody bound in the initial step. The color development is stopped and the intensity of the color is measured.
Applications
Used for the quantitative determination of Anti-Pembrolizumab Neutralizing Antibody concentration in serum and plasma.
Detection method
Colorimetric
Sample type
Plasma, Serum
Assay type
Quantitative
Range
7.81 - 500 ng/mL
Sensitivity
6.90 ng/mL
Precision
Intra-Assay Precision (Precision within an assay): <20%
Three samples of known concentration were tested sixteen times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): <20%
Three samples of known concentration were tested in twenty four separate assays to assess inter-assay precision.
|
|
Intra-Assay Precision |
Inter-Assay Precision |
||||
|
Sample |
1 |
2 |
3 |
1 |
2 |
3 |
|
n |
16 |
16 |
16 |
24 |
24 |
24 |
|
Mean (ng/mL) |
292.6 |
78.7 |
19.0 |
293.1 |
75.5 |
21.7 |
|
Standard deviation |
15.7 |
7.4 |
3.7 |
27.1 |
4.2 |
3.3 |
|
CV (%) |
5.4 |
9.3 |
19.5 |
9.2 |
5.6 |
15.1 |
Recovery
80-120%
Shipping
2-8 ℃
Stability and Storage
When the kit was stored at the recommended temperature for 6 months, the signal intensity decreased by less than 20%.
Alternative Names
MK-3475, CAS: 1374853-91-4
For research use only. Not for human or drug use.
