Catalog No.
PHA18101
Species reactivity
Human
Host species
Rabbit
Isotype
IgG
Clonality
Polyclonal
Immunogen
E. coli - derived recombinant Human E2F3 (Ser174-Gly250).
Applications
ELISA, IHC, WB
Recommended Dilution
| Application |
Dilution |
| ELISA |
1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000 |
Target
E2F-3, KIAA0075, Transcription factor E2F3, E2F3
Purification
Purified by antigen affinity column.
Accession
O00716
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Note
For research use only.
Deregulated expression of E2F family members induces S-phase entry and overcomes p16INK4A-mediated growth suppression., PMID:8622649
E2F-4 switches from p130 to p107 and pRB in response to cell cycle reentry., PMID:8657117
BRCA1 proteins are transported to the nucleus in the absence of serum and splice variants BRCA1a, BRCA1b are tyrosine phosphoproteins that associate with E2F, cyclins and cyclin dependent kinases., PMID:9244350
pRb and p107 regulate E2F activity during lens fiber cell differentiation., PMID:9467965
Modulation of E2F activity in primary mouse B cells following stimulation via surface IgM and CD40 receptors., PMID:10540350
Expression of the E2F and retinoblastoma families of proteins during neural differentiation., PMID:11275408
MMP expression profiling in recurred stage IB lung cancer., PMID:14647437
Direct binding of apoptosis signal-regulating kinase 1 to retinoblastoma protein: novel links between apoptotic signaling and cell cycle machinery., PMID:15210709
Inactivation of the Rb pathway and overexpression of both isoforms of E2F3 are obligate events in bladder tumours with 6p22 amplification., PMID:18037967
Gene Ontology-driven transcriptional analysis of CD34+ cell-initiated megakaryocytic cultures identifies new transcriptional regulators of megakaryopoiesis., PMID:18252802
Opposite effects of interleukin-1alpha and transforming growth factor-beta2 induce stage-specific regulation of junctional adhesion molecule-B gene in Sertoli cells., PMID:19164472
Identification of a preneoplastic gene expression profile in tubal epithelium of BRCA1 mutation carriers., PMID:21170264
An E2F1-mediated DNA damage response contributes to the replication of human cytomegalovirus., PMID:21589897
EGFR signals downregulate tumor suppressors miR-143 and miR-145 in Western diet-promoted murine colon cancer: role of G1 regulators., PMID:21653642
The TAg-RB murine retinoblastoma cell of origin has immunohistochemical features of differentiated Muller glia with progenitor properties., PMID:21862643
Noise induced changes in the expression of p38/MAPK signaling proteins in the sensory epithelium of the inner ear., PMID:21871588
E2F8 as a Novel Therapeutic Target for Lung Cancer., PMID:26089541
Alcoholic hepatitis versus non-alcoholic steatohepatitis: Levels of expression of some proteins involved in tumorigenesis., PMID:29307797
Molecular pathology of the luminal class of urothelial tumors., PMID:31232464
Understanding of cell death induced by the constituents of Taxus yunnanensis wood., PMID:35428370
Interplay between Tumor Mutational Burden and Mutational Profile and Its Effect on Overall Survival: A Pilot Study of Metastatic Patients Treated with Immune Checkpoint Inhibitors., PMID:36358851
For research use only. Not for human or drug use.
