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InVivoMAb Anti-Rotavirus A VP4/Outer capsid protein VP4 Antibody (Iv0200)

Catalog #:   VVV20202 Specific References (50) DATASHEET
Host species: Human
Isotype: IgG1, lambda
Applications: Blocking, ELISA
Accession: Q2VE61
Overview

Catalog No.

VVV20202

Species reactivity

Rotavirus A

Host species

Human

Isotype

IgG1, lambda

Clonality

Monoclonal

Target

Outer capsid protein VP4, VP4

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

Q2VE61

Applications

Blocking, ELISA

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

Iv0200

Data Image
  • Bioactivity
    Detects Rotavirus A, Rotavirus A (strain RVA/Human/United States/Wa/1974/G1P1A[8]) (RV-A) VP4/Outer capsid protein VP4 in indirect ELISAs.
  • WESTERN BLOT
    Various lysates were subjected to SDS PAGE followed by western blot with Rotavirus A VP4/Outer capsid protein VP4 antibody (VVV20202) at 1µg/ml.

    Lane 1: Rotavirus A VP4 transfected HEK293 cell lysate
    Lane 2: Non-transfected HEK293 cell lysate

    Second Ab: Goat Anti-Human IgG H&L Polyclonal antibody, HRP (PHB96431) at 0.1 μg/mL.

    Predict MW: 19 kDa
    Observed MW: 19 kDa
References

A rotavirus VP4 or VP7 monoreassortant panel identifies genotypes that are less susceptible to neutralization by systemic antibodies induced by vaccination or natural infection., PMID:40444468

Truncated rotavirus VP4 proteins induce stronger protective immunity compared to P2 - VP8 in animal models., PMID:40194664

VP4-Specific IgA level as a correlate of neutralizing antibody and fecal shedding of porcine rotavirus infection., PMID:40179488

Breast milk delivery of an engineered dimeric IgA protects neonates against rotavirus., PMID:39842610

A single residue switch mediates the broad neutralization of Rotaviruses., PMID:39833145

Chimeric Viruses Enable Study of Antibody Responses to Human Rotaviruses in Mice., PMID:39066309

The synergy of recombinant NSP4 and VP4 from porcine rotavirus elicited a strong mucosal response., PMID:38850894

Antigenic epitope analysis of Pakistani G3 and G9 rotavirus strains compared to vaccine strains revealed multiple amino acid differences., PMID:38759540

Wa-VP4* as a candidate rotavirus vaccine induced homologous and heterologous virus neutralizing antibody responses in mice, pigs, and cynomolgus monkeys., PMID:38670845

Recombinant bivalent subunit vaccine combining truncated VP4 from P[7] and P[23] induces protective immunity against prevalent porcine rotaviruses., PMID:38591886

Lactobacillus acidophilus Expressing Murine Rotavirus VP8 and Mucosal Adjuvants Induce Virus-Specific Immune Responses., PMID:38140179

Interaction of species A rotavirus VP4 with the cellular proteins vimentin and actin related protein 2 discovered by a proximity interactome assay., PMID:37991368

Generation and characterization of mouse monoclonal antibodies against the VP4 protein of group A human rotaviruses., PMID:36152816

Phylogenetic analysis of the viral proteins VP4/VP7 of circulating human rotavirus strains in China from 2016 to 2019 and comparison of their antigenic epitopes with those of vaccine strains., PMID:36004332

Rotavirus VP4 Epitope of a Broadly Neutralizing Human Antibody Defined by Its Structure Bound with an Attenuated-Strain Virion., PMID:35924923

Monoreassortant Rotaviruses of Multiple G Types Are Differentially Neutralized by Sera From Infants Vaccinated With ROTARIX and RotaTeq., PMID:34628500

Reverse Genetics Approach for Developing Rotavirus Vaccine Candidates Carrying VP4 and VP7 Genes Cloned from Clinical Isolates of Human Rotavirus., PMID:33087468

In Silico Characterization of Epitopes from Human Rotavirus VP7 Genotype G9 Design for Vaccine Development., PMID:32245310

VP7 and VP4 genotypes of rotaviruses cocirculating in Iran, 2015 to 2017: Comparison with cogent sequences of Rotarix and RotaTeq vaccine strains before their use for universal mass vaccination., PMID:31774174

[Polymerization and evaluation of the protective efficacy of rotavirus VP4* proteins]., PMID:30806058

The C Terminus of Rotavirus VP4 Protein Contains an Actin Binding Domain Which Requires Cooperation with the Coiled-Coil Domain for Actin Remodeling., PMID:30333172

Multiple Introductions and Antigenic Mismatch with Vaccines May Contribute to Increased Predominance of G12P[8] Rotaviruses in the United States., PMID:30333170

Design and testing of a highly conserved human rotavirus VP8* immunogenic peptide with potential for vaccine development., PMID:29886031

Expression and characterization of a novel truncated rotavirus VP4 for the development of a recombinant rotavirus vaccine., PMID:29555220

Activation of PI3K, Akt, and ERK during early rotavirus infection leads to V-ATPase-dependent endosomal acidification required for uncoating., PMID:29352319

VP4- and VP7-specific antibodies mediate heterotypic immunity to rotavirus in humans., PMID:28637924

Expression of bovine rotavirus VP8 and preparation of IgY antibodies against recombinant VP8., PMID:28523920

Engineering and expression of a human rotavirus candidate vaccine in Nicotiana benthamiana., PMID:26626122

Modeling of the rotavirus group C capsid predicts a surface topology distinct from other rotavirus species., PMID:26524514

Rotavirus VP6 preparations as a non-replicating vaccine candidates., PMID:26021725

VP7 of Rhesus monkey rotavirus RRV contributes to diabetes acceleration in association with an elevated anti-rotavirus antibody response., PMID:25262468

Structural characterization by multistage mass spectrometry (MSn) of human milk glycans recognized by human rotaviruses., PMID:25048706

Human milk contains novel glycans that are potential decoy receptors for neonatal rotaviruses., PMID:25048705

A new rotavirus VP6-based foreign epitope presenting vector and immunoreactivity of VP4 epitope chimeric proteins., PMID:24702286

Expression of human rotavirus chimeric fusion proteins from replicating but non disseminating adenovectors and elicitation of rotavirus-specific immune responses in mice., PMID:23430460

Development of neutralizing monoclonal antibodies against VP4 of rotavirus CC0812-1., PMID:22894782

Genetic analyses reveal differences in the VP7 and VP4 antigenic epitopes between human rotaviruses circulating in Belgium and rotaviruses in Rotarix and RotaTeq., PMID:22189107

Evidence of conserved epitopes in variable region of VP8* subunit of VP4 protein of rotaviruses of P[8]-1 and P[8]-3 lineages., PMID:21978162

Cross-linking of rotavirus outer capsid protein VP7 by antibodies or disulfides inhibits viral entry., PMID:21849465

Production of human rotavirus and Salmonella antigens in plants and elicitation of fljB-specific humoral responses in mice., PMID:20725806

Antigenic and molecular characterization of unusual rotavirus strains in Burkina Faso in 1999., PMID:20684707

Characterization of human rotavirus strains from children with diarrhea in Nairobi and Kisumu, Kenya, between 2000 and 2002., PMID:20684701

Oral vaccination with the porcine rotavirus VP4 outer capsid protein expressed by Lactococcus lactis induces specific antibody production., PMID:20625406

Identification of G8 rotavirus strains determined as G12 by rotavirus genotyping PCR: updating the current genotyping methods., PMID:20138804

A rotavirus spike protein conformational intermediate binds lipid bilayers., PMID:20007281

Recombinant porcine rotavirus VP4 and VP4-LTB expressed in Lactobacillus casei induced mucosal and systemic antibody responses in mice., PMID:19958557

Resistance to rotavirus infection in adult volunteers challenged with a virulent G1P1A[8] virus correlated with serum immunoglobulin G antibodies to homotypic viral proteins 7 and 4., PMID:19785527

Structure of rotavirus outer-layer protein VP7 bound with a neutralizing Fab., PMID:19520960

A longitudinal cohort study in calves evaluated for rotavirus infections from 1 to 12 months of age by sequential serological assays., PMID:19343476

Cloning and expression of simian rotavirus spike protein (VP4) in insect cells by baculovirus expression system., PMID:19252673

Datasheet
$ 372
Product specifications
100 μg 372 1 mg 1486

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InVivoMAb Anti-Rotavirus A VP4/Outer capsid protein VP4 Antibody (Iv0200) [VVV20202]
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