Catalog No.
MVV28401
Species reactivity
Classical swine fever virus (CSFV)
Host species
Mouse
Isotype
IgG2b
Clonality
Monoclonal
Immunogen
Recombinant CSFV NS5B/RNA-directed RNA polymerase protein.
Target
polyprotein, polyprotein
Concentration
1 mg/ml
Endotoxin level
Please contact with the lab for this information.
Purity
>95% as determined by SDS-PAGE.
Purification
Protein A/G purified from cell culture supernatant.
Accession
AAT85717
Applications
ELISA, WB
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Clone ID
1A014
The classic swine fever virus (CSFV) core protein can enhance de novo-initiated RNA synthesis by the CSFV polymerase NS5B. PMID: 24824302
Characterization of RNA-dependent RNA polymerase activity of CSFV NS5B proteins expressed in Escherichia coli. PMID: 12913359
Porcine Viperin protein inhibits the replication of classical swine fever virus (CSFV) in vitro. PMID: 29061156
In vitro inhibition of CSFV replication by multiple siRNA expression. PMID: 21699919
Crystal Structure of Classical Swine Fever Virus NS5B Reveals a Novel N-Terminal Domain. PMID: 29720518
Genome and molecular characterization of a CSFV strain isolated from a CSF outbreak in South China. PMID: 23296074
Porcine Mx1 Protein Inhibits Classical Swine Fever Virus Replication by Targeting Nonstructural Protein NS5B. PMID: 29343573
Characterisation of interaction between NS3 and NS5B protein of classical swine fever virus by deletion of terminal sequences of NS5B. PMID: 21237222
Characterization of NS3, NS5A and NS5B of classical swine fever virus through mutation and complementation analysis. PMID: 19713054
Classical swine fever virus NS5A regulates viral RNA replication through binding to NS5B and 3'UTR. PMID: 22795973
Specific interaction between the classical swine fever virus NS5B protein and the viral genome. PMID: 15373834
Classical swine fever virus NS5B-GFP fusion protein possesses an RNA-dependent RNA polymerase activity. PMID: 12209316
Classical swine fever virus NS5B protein suppresses the inhibitory effect of NS5A on viral translation by binding to NS5A. PMID: 22258858
Proline to Threonine Mutation at Position 162 of NS5B of Classical Swine Fever Virus Vaccine C Strain Promoted Genome Replication and Infectious Virus Production by Facilitating Initiation of RNA Synthesis. PMID: 34452387
Development and Application of RAA Nucleic Acid Test Strip Assay and Double RAA Gel Electrophoresis Detection Methods for ASFV and CSFV. PMID: 35174210
Classical swine fever virus NS3 is an IRES-binding protein and increases IRES-dependent translation. PMID: 20637813
Episodic adaptive diversification of classical swine fever virus RNA-dependent RNA polymerase NS5B. PMID: 26485449
Genetic typing of classical swine fever virus. PMID: 10785324
Classical swine fever virus NS3 enhances RNA-dependent RNA polymerase activity by binding to NS5B. PMID: 19951725
Classical Swine Fever Virus Infection Induces Endoplasmic Reticulum Stress-Mediated Autophagy to Sustain Viral Replication in vivo and in vitro. PMID: 31798542
In vitro inhibition of classical swine fever virus replication by siRNAs targeting Npro and NS5B genes. PMID: 18262291
Molecular characterization of classical swine fever virus isolates from India during 2012-14. PMID: 28279702
Genetic variation in the 5' end and NS5B regions of classical swine fever virus genome among Japanese isolates. PMID: 10385204
Characterization of classical swine fever virus (CSFV) nonstructural protein 3 (NS3) helicase activity and its modulation by CSFV RNA-dependent RNA polymerase. PMID: 19185595
Phylogenetic analysis of recent classical swine fever virus (CSFV) isolates from Assam, India. PMID: 19896713
RNA-dependent RNA polymerase activity of Classical swine fever virus NS5B protein expressed in natural host cells. PMID: 14524473
Determination of Critical Requirements for Classical Swine Fever Virus NS2-3-Independent Virion Formation. PMID: 31292243
Classical swine fever virus NS5A protein interacts with 3'-untranslated region and regulates viral RNA synthesis. PMID: 22261205
The ESCRT-I Subunit Tsg101 Plays Novel Dual Roles in Entry and Replication of Classical Swine Fever Virus. PMID: 33328308
[Research Progress in the Core Proteins of the Classical Swine Fever Virus]. PMID: 26738299
A unique intra-molecular fidelity-modulating mechanism identified in a viral RNA-dependent RNA polymerase. PMID: 30239956
Biosynthesis of classical swine fever virus nonstructural proteins. PMID: 21270154
Mutational analysis of the GDD sequence motif of classical swine fever virus RNA-dependent RNA polymerases. PMID: 16917742
Application of real-time quantitative polymerase chain reaction to monitoring infection of classic swine fever virus and determining optimal harvest time in large-scale production. PMID: 24021314
Effect of NS3 and NS5B proteins on classical swine fever virus internal ribosome entry site-mediated translation and its host cellular translation. PMID: 18343841
Intra-host variation structure of classical swine fever virus NS5B in relation to antiviral therapy. PMID: 23511203
Predominance of genotype 1.1 and emergence of genotype 2.2 classical swine fever viruses in north-eastern region of India. PMID: 25135466
Classical swine fever virus diversity and evolution. PMID: 8683221
Complete genome sequence of attenuated low-temperature Thiverval strain of classical swine fever virus. PMID: 18401695
Genetic clustering of recent classical swine fever virus isolates from Karnataka, India revealed the emergence of subtype 2.2 replacing subtype 1.1. PMID: 26396984
Genome comparison of a novel classical swine fever virus isolated in China in 2004 with other CSFV strains. PMID: 16972026
Genotyping and Molecular Characterization of Classical Swine Fever Virus Isolated in China during 2016-2018. PMID: 33921513
The GTP binding sites interacted with RNA-dependent RNA polymerase of classical swine fever virus in de novo initiation. PMID: 18430987
Characterization of the swine U6 promoter for short hairpin RNA expression and its application to inhibition of virus replication. PMID: 23916945
Analysis of classical swine fever virus RNA replication determinants using replicons. PMID: 23580431
Imidazo[4,5-c]pyridines inhibit the in vitro replication of the classical swine fever virus and target the viral polymerase. PMID: 17997169
Complete Genome Sequence of a Sub-Subgenotype 2.1i Isolate of Classical Swine Fever Virus from China. PMID: 28385846
De novo RNA synthesis and homology modeling of the classical swine fever virus RNA polymerase. PMID: 16022897
Phylogenetic analysis of NS5B gene of classical swine fever virus isolates indicates plausible Chinese origin of Indian subgroup 2.2 viruses. PMID: 21246270
Origin and evolution of viruses causing classical swine fever in Cuba. PMID: 15878213
Phylogenetic comparison and molecular epidemiology of classical swine fever virus. PMID: 10595410
Sequence adaptations during growth of rescued classical swine fever viruses in cell culture and within infected pigs. PMID: 27527774
Development of a loop-mediated isothermal amplification for visual detection of the HCLV vaccine against classical swine fever in China. PMID: 21055419
Molecular epidemiology of classical swine fever in the Russian Federation. PMID: 14633204
Substituted 2,6-bis(benzimidazol-2-yl)pyridines: a novel chemical class of pestivirus inhibitors that targets a hot spot for inhibition of pestivirus replication in the RNA-dependent RNA polymerase. PMID: 24680957
Classical swine fever virus is genetically stable in vitro and in vivo. PMID: 10542017
Emergence of 2.1. subgenotype of classical swine fever virus in pig population of India in 2011. PMID: 25365116
Validation of a loop-mediated isothermal amplification assay for visualised detection of wild-type classical swine fever virus. PMID: 20304010
Classical swine fever virus: a second ring test to evaluate RT-PCR detection methods. PMID: 11042401
A multiplex RT-PCR assay for the rapid and differential diagnosis of classical swine fever and other pestivirus infections. PMID: 19577384
Full-length GBV-C/HGV genomes from nine Japanese isolates: characterization by comparative analyses. PMID: 9687865
Genetic analysis of classical swine fever virus isolates from a small geographic area. PMID: 9091287
For research use only. Not for human or drug use.
