Catalog No.
YMN00501
Expression system
E. coli
Species
Mus musculus (Mouse)
Protein length
Gln38-Lys250
Predicted molecular weight
26.81 kDa
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Accession
Q9D259
Applications
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol.
Reconstitution
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternative Names
Cysteine-rich secretory protein, Cysteine-rich secretory protein 4, Crisp4
Mammalian cysteine-rich secretory proteins interact with plasma membrane Ca2+ exporter PMCA4b., PMID:37882330
CRISPs Function to Boost Sperm Power Output and Motility., PMID:34422816
Functional redundancy and compensation: Deletion of multiple murine Crisp genes reveals their essential role for male fertility., PMID:33037689
Expression and purification of recombinant mouse CRISP4 using a baculovirus system., PMID:31759086
Relevance of CRISP proteins for epididymal physiology, fertilization, and fertility., PMID:31218833
Impaired male fertility and abnormal epididymal epithelium differentiation in mice lacking CRISP1 and CRISP4., PMID:30510210
Epididymal cysteine-rich secretory proteins are required for epididymal sperm maturation and optimal sperm function., PMID:29361143
Obesity-Induced Infertility in Male Mice Is Associated With Disruption of Crisp4 Expression and Sperm Fertilization Capacity., PMID:28911169
Human fertilization: epididymal hCRISP1 mediates sperm-zona pellucida binding through its interaction with ZP3., PMID:24334245
Loss of cysteine-rich secretory protein 4 (Crisp4) leads to deficiency in sperm-zona pellucida interaction in mice., PMID:21865554
Cysteine-rich secretory protein 4 is an inhibitor of transient receptor potential M8 with a role in establishing sperm function., PMID:21482758
Identification of rat cysteine-rich secretory protein 4 (Crisp4) as the ortholog to human CRISP1 and mouse Crisp4., PMID:16467491
Mouse cysteine-rich secretory protein 4 (CRISP4): a member of the Crisp family exclusively expressed in the epididymis in an androgen-dependent manner., PMID:15673606