Catalog No.
YHK91201
Expression system
E. coli
Species
Homo sapiens (Human)
Protein length
Ser94-Gly146
Predicted molecular weight
34.59 kDa
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Accession
Q9H2W2
Applications
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol.
Reconstitution
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternative Names
MIXL, MIXL1, Homeobox protein MIXL1, hMix, Mix.1 homeobox-like protein, MIX1 homeobox-like protein 1, Homeodomain protein MIX
Brachyury and related Tbx proteins interact with the Mixl1 homeodomain protein and negatively regulate Mixl1 transcriptional activity. PMID: 22164283
Transcriptional activation by the Mixl1 homeodomain protein in differentiating mouse embryonic stem cells. PMID: 19711456
A conditional mutant allele for analysis of Mixl1 function in the mouse. PMID: 24596343
A human MIXL1 green fluorescent protein reporter embryonic stem cell line engineered using TALEN-based genome editing. PMID: 27558609
Mixl1 localizes to putative axial stem cell reservoirs and their posterior descendants in the mouse embryo. PMID: 24632399
Transcriptional regulation of the homeobox gene Mixl1 by TGF-beta and FoxH1. PMID: 15982639
Pdgfrα and Flk1 are direct target genes of Mixl1 in differentiating embryonic stem cells. PMID: 22265737
A role for BMP-induced homeobox gene MIXL1 in acute myelogenous leukemia and identification of type I BMP receptor as a potential target for therapy. PMID: 25544748
Mixl1 and oct4 proteins are transiently co-expressed in differentiating mouse and human embryonic stem cells. PMID: 16433620
Amino terminal tyrosine phosphorylation of human MIXL1. PMID: 17224082
Mixl1 is required for axial mesendoderm morphogenesis and patterning in the murine embryo. PMID: 12117810
Foxh1 recruits Gsc to negatively regulate Mixl1 expression during early mouse development. PMID: 17568773
Differential expression of the human MIXL1 gene product in non-Hodgkin and Hodgkin lymphomas. PMID: 17303500
Enforced expression of the homeobox gene Mixl1 impairs hematopoietic differentiation and results in acute myeloid leukemia. PMID: 17060613
Enforced expression of Mixl1 during mouse ES cell differentiation suppresses hematopoietic mesoderm and promotes endoderm formation. PMID: 19038793
Targeting a GFP reporter gene to the MIXL1 locus of human embryonic stem cells identifies human primitive streak-like cells and enables isolation of primitive hematopoietic precursors. PMID: 18032708
The primitive streak gene Mixl1 is required for efficient haematopoiesis and BMP4-induced ventral mesoderm patterning in differentiating ES cells. PMID: 15673572
Mixl1 and Flk1 Are Key Players of Wnt/TGF-β Signaling During DMSO-Induced Mesodermal Specification in P19 cells. PMID: 25521758
Targeted organ generation using Mixl1-inducible mouse pluripotent stem cells in blastocyst complementation. PMID: 25192056
The preleukemic state of mice reconstituted with Mixl1-transduced marrow cells. PMID: 18056627
Differentiating embryonic stem cells pass through 'temporal windows' that mark responsiveness to exogenous and paracrine mesendoderm inducing signals. PMID: 20502661
Differential response of epiblast stem cells to Nodal and Activin signalling: a paradigm of early endoderm development in the embryo. PMID: 25349457
A human Mix-like homeobox gene MIXL shows functional similarity to Xenopus Mix.1. PMID: 12070013
TATA box-binding protein-related factor 3 drives the mesendoderm specification of human embryonic stem cells by globally interacting with the TATA box of key mesendodermal genes. PMID: 32448362
Vertebrate development: the fast track to nodal signalling. PMID: 11102828
Glucose as a regulator of eukaryotic gene transcription. PMID: 16269245
In Vivo Regulation of the Zebrafish Endoderm Progenitor Niche by T-Box Transcription Factors. PMID: 28658625
Cell fusion enhances mesendodermal differentiation of human induced pluripotent stem cells. PMID: 25004077
A conserved regulatory program initiates lateral plate mesoderm emergence across chordates. PMID: 31451684
Cell Polarity and Division Symmetry Analyses in Transformed Blood Cells. PMID: 30062418
Role of TRIM33 in Wnt signaling during mesendoderm differentiation. PMID: 28844090
Dissecting the molecular hierarchy for mesendoderm differentiation through a combination of embryonic stem cell culture and RNA interference. PMID: 17446562
miR-27b antagonizes BMP signaling in early differentiation of human induced pluripotent stem cells. PMID: 34615950
APELIN promotes hematopoiesis from human embryonic stem cells. PMID: 22611158
Sequential allocation and global pattern of movement of the definitive endoderm in the mouse embryo during gastrulation. PMID: 17151016
Sumoylation differentially regulates Goosecoid-mediated transcriptional repression. PMID: 18336814
Turning human epidermis into pancreatic endoderm. PMID: 21060974
Biophysical studies of blood plasma proteins; recovery of gamma-globulin from human blood protein mixtures. PMID: 20989473
Microbioreactor arrays for full factorial screening of exogenous and paracrine factors in human embryonic stem cell differentiation. PMID: 23300662
Cell type-specific target selection by combinatorial binding of Smad2/3 proteins and hepatocyte nuclear factor 4alpha in HepG2 cells. PMID: 21646355
Mezzo, a paired-like homeobox protein is an immediate target of Nodal signalling and regulates endoderm specification in zebrafish. PMID: 12397099
Elevated Exogenous Pyruvate Potentiates Mesodermal Differentiation through Metabolic Modulation and AMPK/mTOR Pathway in Human Embryonic Stem Cells. PMID: 31353224
Genome-Wide Identification of MESP1 Targets Demonstrates Primary Regulation Over Mesendoderm Gene Activity. PMID: 26205879
Vitronectin-activated αvβ3 and αvβ5 integrin signalling specifies haematopoietic fate in human pluripotent stem cells. PMID: 33656760
The DUX4 homeodomains mediate inhibition of myogenesis and are functionally exchangeable with the Pax7 homeodomain. PMID: 28935672
The nuclear transporter importin 13 is critical for cell survival during embryonic stem cell differentiation. PMID: 33333437
Long-term expandable SOX9+ chondrogenic ectomesenchymal cells from human pluripotent stem cells. PMID: 25818812
Differentiation of human embryonic stem cells in serum-free medium reveals distinct roles for bone morphogenetic protein 4, vascular endothelial growth factor, stem cell factor, and fibroblast growth factor 2 in hematopoiesis. PMID: 17556598
A poised chromatin platform for TGF-β access to master regulators. PMID: 22196728
Human chondrogenic paraxial mesoderm, directed specification and prospective isolation from pluripotent stem cells. PMID: 22701159
SMYD2 Drives Mesendodermal Differentiation of Human Embryonic Stem Cells Through Mediating the Transcriptional Activation of Key Mesendodermal Genes. PMID: 31348575
Cyclosporin A induces cardiac differentiation but inhibits hemato-endothelial differentiation of P19 cells. PMID: 25629977
MESP1 knock-down in human iPSC attenuates early vascular progenitor cell differentiation after completed primitive streak specification. PMID: 30389344
Analysis of multi-lineage gene expression dynamics during primordial germ cell induction from human induced pluripotent stem cells. PMID: 32127035
Short-term BMP-4 treatment initiates mesoderm induction in human embryonic stem cells. PMID: 18042803
Differential regulation of epiboly initiation and progression by zebrafish Eomesodermin A. PMID: 22142964
Generation of human female reproductive tract epithelium from human embryonic stem cells. PMID: 21698266
STELLA collaborates in distinct mesendodermal cell subpopulations at the fetal-placental interface in the mouse gastrula. PMID: 28322735
Nodal-dependent mesendoderm specification requires the combinatorial activities of FoxH1 and Eomesodermin. PMID: 21637786
Major amino acids in collagen hydrolysate regulate the differentiation of mouse embryoid bodies. PMID: 23623897
Notch signaling can regulate endoderm formation in zebrafish. PMID: 15042699
Mml, a mouse Mix-like gene expressed in the primitive streak. PMID: 10495285
Eomesodermin is a localized maternal determinant required for endoderm induction in zebrafish. PMID: 16198294
Substrate stiffness affects early differentiation events in embryonic stem cells. PMID: 19768669
A synthetic substrate to support early mesodermal differentiation of human embryonic stem cells. PMID: 21821284
Clonal variation of human induced pluripotent stem cells for induction into the germ cell fate. PMID: 28453617
Efficient differentiation of human pluripotent stem cells into mesenchymal stem cells by modulating intracellular signaling pathways in a feeder/serum-free system. PMID: 21793661
A novel stepwise differentiation of functional pancreatic exocrine cells from embryonic stem cells. PMID: 20887097
Zebrafish endoderm formation is regulated by combinatorial Nodal, FGF and BMP signalling. PMID: 16672336
Chemically Defined Conditions Mediate an Efficient Induction of Mesodermal Lineage from Human Umbilical Cord- and Bone Marrow- Mesenchymal Stem Cells and Dental Pulp Pluripotent-Like Stem Cells. PMID: 29412734
miR-124a is important for migratory cell fate transition during gastrulation of human embryonic stem cells. PMID: 20665740
Mixer/Bon and FoxH1/Sur have overlapping and divergent roles in Nodal signaling and mesendoderm induction. PMID: 14522874
Enhancement of insulin-producing cell differentiation from embryonic stem cells using pax4-nucleofection method. PMID: 17461469
Alpha terpineol directs bone marrow mesenchymal stem cells toward neuronal lineage through regulation of wnt signaling pathway. PMID: 36651266
Rapid and efficient differentiation of human pluripotent stem cells into intermediate mesoderm that forms tubules expressing kidney proximal tubular markers. PMID: 24357672
A functional single nucleotide polymorphism in the tyrosinase gene promoter affects skin color and transcription activity in the black-boned chicken. PMID: 29050424
Different Smad2 partners bind a common hydrophobic pocket in Smad2 via a defined proline-rich motif. PMID: 11782434
Acceleration of mesoderm development and expansion of hematopoietic progenitors in differentiating ES cells by the mouse Mix-like homeodomain transcription factor. PMID: 16403910
Structure, upstream promoter region, and functional domains of a mouse and human Mix paired-like homeobox gene. PMID: 12095687
Mouse Mix gene is activated early during differentiation of ES and F9 stem cells and induces endoderm in frog embryos. PMID: 12619131
For research use only. Not for human or drug use.
