Catalog No.
YHK91001
Expression system
E. coli
Species
Homo sapiens (Human)
Protein length
Met1-Gly413
Predicted molecular weight
48.92 kDa
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Accession
Q9Y5B9
Applications
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol.
Reconstitution
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternative Names
Chromatin-specific transcription elongation factor 140 kDa subunit, FACT140, FACTP140, FACTp140, Facilitates chromatin transcription complex subunit SPT16, FACT complex subunit SPT16, FACT 140 kDa subunit, SUPT16H, hSPT16
FACT subunit SUPT16H associates with BRD4 and contributes to silencing of interferon signaling. PMID: 35904816
The FACT complex facilitates expression of lysosomal and antioxidant genes through binding to TFEB and TFE3. PMID: 35230915
FACT subunit SUPT16H associates with BRD4 and contributes to silencing of antiviral interferon signaling. PMID: 33907746
FACT Proteins, SUPT16H and SSRP1, Are Transcriptional Suppressors of HIV-1 and HTLV-1 That Facilitate Viral Latency. PMID: 26378236
Large-scale discovery of novel neurodevelopmental disorder-related genes through a unified analysis of single-nucleotide and copy number variants. PMID: 35468861
Neurodevelopmental phenotype associated with CHD8-SUPT16H duplication. PMID: 31823155
FACT caught in the act of manipulating the nucleosome. PMID: 31775157
De novo variants in SUPT16H cause neurodevelopmental disorders associated with corpus callosum abnormalities. PMID: 31924697
Haematopoietic stem cell-dependent Notch transcription is mediated by p53 through the Histone chaperone Supt16h. PMID: 33230303
Dual targeting of the epigenome via FACT complex and histone deacetylase is a potent treatment strategy for DIPG. PMID: 33852836
Histone chaperone FACT regulates homologous recombination by chromatin remodeling through interaction with RNF20. PMID: 24357716
Histone chaperone FACT complex mediates oxidative stress response to promote liver cancer progression. PMID: 31439637
The H2B ubiquitin ligase RNF40 cooperates with SUPT16H to induce dynamic changes in chromatin structure during DNA double-strand break repair. PMID: 22031019
Novel 14q11.2 microduplication including the CHD8 and SUPT16H genes associated with developmental delay. PMID: 26834018
Recurrent ∼100 Kb microdeletion in the chromosomal region 14q11.2, involving CHD8 gene, is associated with autism and macrocephaly. PMID: 25257502
Validation of a novel shotgun proteomic workflow for the discovery of protein-protein interactions: focus on ZNF521. PMID: 25774781
Structural basis for H2A-H2B recognitions by human Spt16. PMID: 36801613
Naked FACT is unstable. PMID: 23856583
Proteasomal Regulation of Mammalian SPT16 in Controlling Transcription. PMID: 33526453
The mouse Supt16h/Fact140 gene, encoding part of the FACT chromatin transcription complex, maps close to Tcra and is highly expressed in thymus. PMID: 11471063
Human FACT subunits coordinate to catalyze both disassembly and reassembly of nucleosomes. PMID: 36577379
Long term follow-up in a patient with a de novo microdeletion of 14q11.2 involving CHD8. PMID: 25735987
The OTUD5-UBR5 complex regulates FACT-mediated transcription at damaged chromatin. PMID: 30508113
Nek9, a novel FACT-associated protein, modulates interphase progression. PMID: 14660563
A distinct neurodevelopmental syndrome with intellectual disability, autism spectrum disorder, characteristic facies, and macrocephaly is caused by defects in CHD8. PMID: 30670789
Complex mutual regulation of facilitates chromatin transcription (FACT) subunits on both mRNA and protein levels in human cells. PMID: 23839038
Structure of the human histone chaperone FACT Spt16 N-terminal domain. PMID: 26841762
A new small supernumerary marker chromosome involving 14pter → q12 in a child with severe neurodevelopmental retardation: case report and literature review. PMID: 24013083
FACT facilitates transcription-dependent nucleosome alteration. PMID: 12934006
The structural basis of human Spt16 N-terminal domain interaction with histone (H3-H4)2 tetramer. PMID: 30528735
The yeast protein complex containing cdc68 and pob3 mediates core-promoter repression through the cdc68 N-terminal domain. PMID: 9832518
Spt16-Pob3 and the HMG protein Nhp6 combine to form the nucleosome-binding factor SPN. PMID: 11432837
Cutaneous innate immune tolerance is mediated by epigenetic control of MAP2K3 by HDAC8/9. PMID: 34021025
Chromatin remodeller SMARCA4 recruits topoisomerase 1 and suppresses transcription-associated genomic instability. PMID: 26842758
The something about silencing protein, Sas3, is the catalytic subunit of NuA3, a yTAF(II)30-containing HAT complex that interacts with the Spt16 subunit of the yeast CP (Cdc68/Pob3)-FACT complex. PMID: 10817755
The FACT Complex Promotes Avian Leukosis Virus DNA Integration. PMID: 28122976
FACT subunit Spt16 controls UVSSA recruitment to lesion-stalled RNA Pol II and stimulates TC-NER. PMID: 30715484
The CENP-T/-W complex is a binding partner of the histone chaperone FACT. PMID: 27284163
Functions of FACT in Breaking the Nucleosome and Maintaining Its Integrity at the Single-Nucleosome Level. PMID: 30029006
Changing the DNA landscape: putting a SPN on chromatin. PMID: 12596908
Suppressor of Ty 16 promotes lung cancer malignancy and is negatively regulated by miR-1227-5p. PMID: 32860308
Enhanced chromatin dynamics by FACT promotes transcriptional restart after UV-induced DNA damage. PMID: 23973375
Modulation of nucleosome-binding activity of FACT by poly(ADP-ribosyl)ation. PMID: 16682447
Histone methyltransferase SETD2 coordinates FACT recruitment with nucleosome dynamics during transcription. PMID: 23325844
Supt16 Haploinsufficiency Impairs PI3K/AKT/mTOR/Autophagy Pathway in Human Pluripotent Stem Cells Derived Neural Stem Cells. PMID: 36769360
Crystal Structure of Human SSRP1 Middle Domain Reveals a Role in DNA Binding. PMID: 26687053
Histone chaperone FACT coordinates nucleosome interaction through multiple synergistic binding events. PMID: 21969370
The chromatin-specific transcription elongation factor FACT comprises human SPT16 and SSRP1 proteins. PMID: 10421373
FACT-mediated exchange of histone variant H2AX regulated by phosphorylation of H2AX and ADP-ribosylation of Spt16. PMID: 18406329
Facilitates chromatin transcription complex is an "accelerator" of tumor transformation and potential marker and target of aggressive cancers. PMID: 23831030
Human SSRP1 has Spt16-dependent and -independent roles in gene transcription. PMID: 17209051
Dermatan Sulfate Is a Potential Regulator of IgH via Interactions With Pre-BCR, GTF2I, and BiP ER Complex in Pre-B Lymphoblasts. PMID: 34113352
A DNA damage-induced p53 serine 392 kinase complex contains CK2, hSpt16, and SSRP1. PMID: 11239457
The histone H3 Lys 27 demethylase JMJD3 regulates gene expression by impacting transcriptional elongation. PMID: 22713873
Copy number variation analysis in adults with catatonia confirms haploinsufficiency of SHANK3 as a predisposing factor. PMID: 27519580
Comprehensive single cell-resolution analysis of the role of chromatin regulators in early C. elegans embryogenesis. PMID: 25446273
RNA polymerase II elongation factors of Saccharomyces cerevisiae: a targeted proteomics approach. PMID: 12242279
The Paf1 complex physically and functionally associates with transcription elongation factors in vivo. PMID: 11927560
Expression of FACT in mammalian tissues suggests its role in maintaining of undifferentiated state of cells. PMID: 21998152
Functional interaction of general transcription initiation factor TFIIE with general chromatin factor SPT16/CDC68. PMID: 10792464
Defects in SPT16 or POB3 (yFACT) in Saccharomyces cerevisiae cause dependence on the Hir/Hpc pathway: polymerase passage may degrade chromatin structure. PMID: 12524332
Novel deletions of 14q11.2 associated with developmental delay, cognitive impairment and similar minor anomalies in three children. PMID: 17545556
Repairing nucleosomes during transcription. PMID: 14583736
For research use only. Not for human or drug use.
