Catalog No.
YHG30501
Expression system
E. coli
Species
Homo sapiens (Human)
Protein length
Gly246-Lys336
Predicted molecular weight
22.42 kDa
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Accession
Q12837
Applications
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol.
Reconstitution
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternative Names
POU domain, class 4, transcription factor 2, POU4F2, Brn-3B, BRN3B, Brain-3B, Brain-specific homeobox/POU domain protein 3B
The emergence of transcriptional identity in somatosensory neurons. PMID: 31915380
Diversity of intrinsically photosensitive retinal ganglion cells: circuits and functions. PMID: 32965515
Pou4f2-GFP knock-in mouse line: A model for studying retinal ganglion cell development. PMID: 27532212
Pou4f2 knock-in Cre mouse: A multifaceted genetic tool for vision researchers. PMID: 27390513
Transcription factor POU4F2 promotes colorectal cancer cell migration and invasion through hedgehog-mediated epithelial-mesenchymal transition. PMID: 34327778
Substituting mouse transcription factor Pou4f2 with a sea urchin orthologue restores retinal ganglion cell development. PMID: 26962139
Pou4f1 and pou4f2 are dispensable for the long-term survival of adult retinal ganglion cells in mice. PMID: 24736625
Two transcription factors, Pou4f2 and Isl1, are sufficient to specify the retinal ganglion cell fate. PMID: 25775587
Epitope-tagging Math5 and Pou4f2: new tools to study retinal ganglion cell development in the mouse. PMID: 19459208
The Wilms' tumor suppressor Wt1 encodes a transcriptional activator of the class IV POU-domain factor Pou4f2 (Brn-3b). PMID: 12609742
Comparative expression analysis of POU4F1, POU4F2 and ISL1 in developing mouse cochleovestibular ganglion neurons. PMID: 24709358
Linking metabolic dysfunction with cardiovascular diseases: Brn-3b/POU4F2 transcription factor in cardiometabolic tissues in health and disease. PMID: 33712567
Eomesodermin, a target gene of Pou4f2, is required for retinal ganglion cell and optic nerve development in the mouse. PMID: 18077589
No association between POU4F1, POU4F2, ISL1 polymorphisms and normal-tension glaucoma. PMID: 32597291
Gene regulation logic in retinal ganglion cell development: Isl1 defines a critical branch distinct from but overlapping with Pou4f2. PMID: 18460603
The POU4F2/Brn-3b transcription factor is required for the hypertrophic response to angiotensin II in the heart. PMID: 31413277
HOXA9, PCDH17, POU4F2, and ONECUT2 as a Urinary Biomarker Combination for the Detection of Bladder Cancer in Chinese Patients with Hematuria. PMID: 30309818
Isl1 and Pou4f2 form a complex to regulate target genes in developing retinal ganglion cells. PMID: 24643061
POU4F2/Brn-3b transcription factor is associated with survival and drug resistance in human ovarian cancer cells. PMID: 30613365
Hsp-27 induction requires POU4F2/Brn-3b TF in doxorubicin-treated breast cancer cells, whereas phosphorylation alters its cellular localisation following drug treatment. PMID: 21279488
An epigenetic biomarker combination of PCDH17 and POU4F2 detects bladder cancer accurately by methylation analyses of urine sediment DNA in Han Chinese. PMID: 26700620
Profound hyperglycemia in knockout mutant mice identifies novel function for POU4F2/Brn-3b in regulating metabolic processes. PMID: 26670484
Diagnosis of bladder cancer recurrence based on urinary levels of EOMES, HOXA9, POU4F2, TWIST1, VIM, and ZNF154 hypermethylation. PMID: 23056278
Essential but partially redundant roles for POU4F1/Brn-3a and POU4F2/Brn-3b transcription factors in the developing heart. PMID: 28594399
Co-expression of POU4F2/Brn-3b with p53 may be important for controlling expression of pro-apoptotic genes in cardiomyocytes following ischaemic/hypoxic insults. PMID: 25356872
Targeting Brn-3b in breast cancer therapy. PMID: 16441225
Identification of an N-terminal transcriptional activation domain within Brn3b/POU4f2. PMID: 15733064
Proliferation-associated POU4F2/Brn-3b transcription factor expression is regulated by oestrogen through ERα and growth factors via MAPK pathway. PMID: 21241485
A Novel Reporter Mouse Uncovers Endogenous Brn3b Expression. PMID: 31197108
Divergent projection patterns of M1 ipRGC subtypes. PMID: 29888785
Discrete gene sets depend on POU domain transcription factor Brn3b/Brn-3.2/POU4f2 for their expression in the mouse embryonic retina. PMID: 14973295
Two new genetically modified mouse alleles labeling distinct phases of retinal ganglion cell development by fluorescent proteins. PMID: 32741043
Developmental expression of the POU domain transcription factor Brn-3b (Pou4f2) in the lateral line and visual system of zebrafish. PMID: 15042710
The Wilms' tumor gene Wt1 is required for normal development of the retina. PMID: 11889045
Improved urine DNA methylation panel for early bladder cancer detection. PMID: 35241014
Activation and repression of gene expression by POU family transcription factors. PMID: 8735273
Role of the Brn-3 family of POU-domain genes in the development of the auditory/vestibular, somatosensory, and visual systems. PMID: 9598366
Transient Expression of Fez Family Zinc Finger 2 Protein Regulates the Brn3b Gene in Developing Retinal Ganglion Cells. PMID: 26861874
Onecut 1 and Onecut 2 are potential regulators of mouse retinal development. PMID: 21830221
Predicting the Lung Squamous Cell Carcinoma Diagnosis and Prognosis Markers by Unique DNA Methylation and Gene Expression Profiles. PMID: 31710242
Quick Commitment and Efficient Reprogramming Route of Direct Induction of Retinal Ganglion Cell-like Neurons. PMID: 33096050
Differences Between Sorafenib and Lenvatinib Treatment from Genetic and Clinical Perspectives for Patients with Hepatocellular Carcinoma. PMID: 35473892
Characterization of multiple bistratified retinal ganglion cells in a purkinje cell protein 2-Cre transgenic mouse line. PMID: 23224947
Mediation by DNA methylation on the association of BMI and serum uric acid in Chinese monozygotic twins. PMID: 36243213
Retinal ganglion cell defects cause decision shifts in visually evoked defense responses. PMID: 32997561
Single cell transcriptomics reveals lineage trajectory of retinal ganglion cells in wild-type and Atoh7-null retinas. PMID: 33674582
Brn3 transcription factors control terminal osteoclastogenesis. PMID: 17668438
Fertility Relevance Probability Analysis Shortlists Genetic Markers for Male Fertility Impairment. PMID: 33238277
Dynamic expression of transcription factor Brn3b during mouse cranial nerve development. PMID: 26356988
De novo genesis of retinal ganglion cells by targeted expression of Klf4 in vivo. PMID: 31405994
Transcriptome of Atoh7 retinal progenitor cells identifies new Atoh7-dependent regulatory genes for retinal ganglion cell formation. PMID: 24799426
Neuroprotective effects of transcription factor Brn3b in an ocular hypertension rat model of glaucoma. PMID: 25587060
Activation of the alpha-internexin promoter by the Brn-3a transcription factor is dependent on the N-terminal region of the protein. PMID: 7852360
Simultaneous co-delivery of neuroprotective drugs from multi-loaded PLGA microspheres for the treatment of glaucoma. PMID: 30664980
Photoentrainment and pupillary light reflex are mediated by distinct populations of ipRGCs. PMID: 21765429
Classical Photoreceptors Are Primarily Responsible for the Pupillary Light Reflex in Mouse. PMID: 27295136
nGnG Amacrine Cells and Brn3b-negative M1 ipRGCs are Specifically Labeled in the ChAT-ChR2-EYFP Mouse. PMID: 32049344
Enhanced Stem Cell Differentiation and Immunopurification of Genome Engineered Human Retinal Ganglion Cells. PMID: 29024560
Generation of retinal ganglion cells with functional axons from human induced pluripotent stem cells. PMID: 25666360
Correction: Proliferation-associated Brn-3b transcription factor can activate cyclin D1 expression in neuroblastoma and breast cancer cells. PMID: 36759573
Cell-specific regulation of the pro-survival Brn-3b transcription factor by microRNAs. PMID: 20609388
Genetic interactions between Brn3 transcription factors in retinal ganglion cell type specification. PMID: 24116103
Brn-3a/POU4F1 interacts with and differentially affects p73-mediated transcription. PMID: 18421303
Brn3a and Brn3b knockout mice display unvaried retinal fine structure despite major morphological and numerical alterations of ganglion cells. PMID: 27391320
The transcriptional regulator BCL6 participates in the secondary gene regulatory response to vitamin D. PMID: 25482012
Neuroprotective effects of Acer palmatum thumb. leaf extract (KIOM-2015E) against ischemia/reperfusion-induced injury in the rat retina. PMID: 33088173
Transplantation of Retinal Ganglion Cells Derived from Male Germline Stem Cell as a Potential Treatment to Glaucoma. PMID: 31580778
CD24 and PRAME Are Novel Grading and Prognostic Indicators for Pineal Parenchymal Tumors of Intermediate Differentiation. PMID: 31567202
Regulation of Brn3b by DLX1 and DLX2 is required for retinal ganglion cell differentiation in the vertebrate retina. PMID: 28356311
Loss of foxc1 in zebrafish reduces optic nerve size and cell number in the retinal ganglion cell layer. PMID: 30684501
Neuronal transcriptional repressor REST suppresses an Atoh7-independent program for initiating retinal ganglion cell development. PMID: 20969844
Differentiation of human ESCs to retinal ganglion cells using a CRISPR engineered reporter cell line. PMID: 26563826
ISL1 and BRN3B co-regulate the differentiation of murine retinal ganglion cells. PMID: 18434421
Differential expression of Brn3 transcription factors in intrinsically photosensitive retinal ganglion cells in mouse. PMID: 21935940
Requirement for math5 in the development of retinal ganglion cells. PMID: 11156601
Genetic labelling of specific axonal pathways in the mouse central nervous system. PMID: 11906522
Cardiac expression of Brn-3a and Brn-3b POU transcription factors and regulation of Hsp27 gene expression. PMID: 18368538
Combinatorial expression of Brn3 transcription factors in somatosensory neurons: genetic and morphologic analysis. PMID: 22262898
The Brn-3c transcription factor contains a neuronal-specific activation domain. PMID: 9579678
Transcription Factor Brn-3b Overexpression Enhances Neurite Outgrowth in PC12 Cells Under Condition of Hypoxia. PMID: 25786379
Transcription factors SOX4 and SOX11 function redundantly to regulate the development of mouse retinal ganglion cells. PMID: 23649630
Brn-3b inhibits generation of amacrine cells by binding to and negatively regulating DLX1/2 in developing retina. PMID: 21875655
Brn3a/Pou4f1 regulates dorsal root ganglion sensory neuron specification and axonal projection into the spinal cord. PMID: 22326227
Post-transcriptional regulation of the Brn-3b transcription factor in differentiating neuroblastoma cells. PMID: 17490655
Analysis of optic nerve stroke by retinal Bex expression. PMID: 16541015
Melanopsin-expressing retinal ganglion cell loss and behavioral analysis in the Thy1-CFP-DBA/2J mouse model of glaucoma. PMID: 23729182
Six3 and Six6 Are Jointly Required for the Maintenance of Multipotent Retinal Progenitors through Both Positive and Negative Regulation. PMID: 30485816
Coupling the transcriptional regulatory action of Brn-3b to the cell cycle clock. PMID: 15020847
Doppel expression is regulated by the Brn-3a and Brn-3b transcription factors. PMID: 15094508
Cyclin D1 fine-tunes the neurogenic output of embryonic retinal progenitor cells. PMID: 19416500
Functional role of position 22 in the homeodomain of Brn-3 transcription factors. PMID: 9694219
For research use only. Not for human or drug use.
