Catalog No.
YHK89401
Expression system
E. coli
Species
Homo sapiens (Human)
Protein length
Asn63-Pro188
Predicted molecular weight
17.14 kDa
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Accession
Q66GS9
Applications
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol.
Reconstitution
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternative Names
KIAA0635, Centrosomal protein of 135 kDa, Centrosomal protein 4, Cep135, CEP4, CEP135
Bld10p/Cep135 determines the number of triplets in the centriole independently of the cartwheel. PMID: 36093892
CEP135 isoform dysregulation promotes centrosome amplification in breast cancer cells. PMID: 30811267
Drosophila Cep135/Bld10 maintains proper centriole structure but is dispensable for cartwheel formation. PMID: 22976301
Mutation in CEP135 causing primary microcephaly and subcortical heterotopia. PMID: 32643282
CEP135 associated primary microcephaly-A rare presentation in early second trimester. PMID: 33933664
Genomic and phenotypic delineation of congenital microcephaly. PMID: 30214071
A Short CEP135 Splice Isoform Controls Centriole Duplication. PMID: 26412126
Mitotic Maturation Compensates for Premature Centrosome Splitting and PCM Loss in Human cep135 Knockout Cells. PMID: 35406752
Bld10/Cep135 stabilizes basal bodies to resist cilia-generated forces. PMID: 23115304
Abnormal centrosomal structure and duplication in Cep135-deficient vertebrate cells. PMID: 23864714
Human microcephaly protein CEP135 binds to hSAS-6 and CPAP, and is required for centriole assembly. PMID: 23511974
Interaction of Cep135 with a p50 dynactin subunit in mammalian centrosomes. PMID: 14983524
miR-26b Targets CEP135 Gene to Regulate Nasopharyngeal Carcinoma Proliferation and Migration by NF-κB Pathway. PMID: 36820950
The Human Centriolar Protein CEP135 Contains a Two-Stranded Coiled-Coil Domain Critical for Microtubule Binding. PMID: 27477386
BLD10/CEP135 is a microtubule-associated protein that controls the formation of the flagellum central microtubule pair. PMID: 22898782
Comprehensive review on the molecular genetics of autosomal recessive primary microcephaly (MCPH). PMID: 30086807
A novel function of CEP135 as a platform protein of C-NAP1 for its centriolar localization. PMID: 18851962
Characterization of Cep135, a novel coiled-coil centrosomal protein involved in microtubule organization in mammalian cells. PMID: 11781336
A truncating mutation of CEP135 causes primary microcephaly and disturbed centrosomal function. PMID: 22521416
A homozygous CEP135 mutation is associated with multiple morphological abnormalities of the sperm flagella (MMAF). PMID: 28866084
Cartwheel assembly. PMID: 25047612
The Association of CEP135 rs4865047 and NPY2R rs1902491 Single Nucleotide Polymorphisms (SNPs) with Rapid Progression of Proliferative Diabetic Retinopathy in Patients with Type 1 Diabetes Mellitus. PMID: 30531682
Filamentous polymers induced by overexpression of a novel centrosomal protein, Cep135. PMID: 10842375
Multisite phosphorylation of C-Nap1 releases it from Cep135 to trigger centrosome disjunction. PMID: 24695856
A novel splice site mutation in CEP135 is associated with primary microcephaly in a Pakistani family. PMID: 26657937
How the newborn centriole becomes a mother. PMID: 27223270
The centriolar protein Bld10/Cep135 is required to establish centrosome asymmetry in Drosophila neuroblasts. PMID: 24954048
An update of pathogenic variants in ASPM, WDR62, CDK5RAP2, STIL, CENPJ, and CEP135 underlying autosomal recessive primary microcephaly in 32 consanguineous families from Pakistan. PMID: 32677750
Superresolution characterization of core centriole architecture. PMID: 33533934
WDR8 is a centriolar satellite and centriole-associated protein that promotes ciliary vesicle docking during ciliogenesis. PMID: 26675238
High-confidence cancer patient stratification through multiomics investigation of DNA repair disorders. PMID: 36435816
Molecular genetics of human primary microcephaly: an overview. PMID: 25951892
Deficient adaptation to centrosome duplication defects in neural progenitors causes microcephaly and subcortical heterotopias. PMID: 34237032
CEP proteins: the knights of centrosome dynasty. PMID: 23456457
Conserved molecular interactions in centriole-to-centrosome conversion. PMID: 26595382
Basal body structure and composition in the apicomplexans Toxoplasma and Plasmodium. PMID: 26855772
Autosomal recessive primary microcephalies (MCPH). PMID: 24780602
Molecular evolutionary analysis of human primary microcephaly genes. PMID: 33941077
Stepwise evolution of the centriole-assembly pathway. PMID: 20392737
A modified TurboID approach identifies tissue-specific centriolar components in C. elegans. PMID: 35442950
Primary Autosomal Recessive Microcephalies and Seckel Syndrome Spectrum Disorders – RETIRED CHAPTER, FOR HISTORICAL REFERENCE ONLY. PMID: 20301772
Homozygous mutations in DZIP1 can induce asthenoteratospermia with severe MMAF. PMID: 32051257
Plk4-induced centriole biogenesis in human cells. PMID: 17681131
The 3D architecture and molecular foundations of de novo centriole assembly via bicentrioles. PMID: 34433076
A method of quantifying centrosomes at the single-cell level in human normal and cancer tissue. PMID: 30699045
Drosophila Ana1 is required for centrosome assembly and centriole elongation. PMID: 27206860
Replication analysis for severe diabetic retinopathy. PMID: 22427569
For research use only. Not for human or drug use.
