Catalog No.
PHK06201
Species reactivity
Human
Host species
Rabbit
Isotype
IgG
Clonality
Polyclonal
Immunogen
E. coli - derived recombinant Human PLCZ1 (Ser469-Thr572).
Tested applications
ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000
Target
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1, PLCZ1, Testis-development protein NYD-SP27, Phospholipase C-zeta-1, Phosphoinositide phospholipase C-zeta-1, PLC-zeta-1
Purification
Purified by antigen affinity column.
Accession
Q86YW0
Applications
ELISA, IHC, WB
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
The mammalian sperm factor phospholipase C zeta is critical for early embryo division and pregnancy in humans and mice., PMID:38670547
Antigen unmasking does not improve the visualization of phospholipase C zeta in human spermatozoa., PMID:34893574
Advancing male age differentially alters levels and localization patterns of PLCzeta in sperm and testes from different mouse strains., PMID:33208563
Localisation of phospholipase Cζ1 (PLCZ1) and postacrosomal WW-binding protein (WBP2 N-terminal like) on equine spermatozoa and flow cytometry quantification of PLCZ1 and association with cleavage in vitro., PMID:31597592
Proteomic Analysis of Dpy19l2-Deficient Human Globozoospermia Reveals Multiple Molecular Defects., PMID:31424156
New Insights into the Molecular Events of Mammalian Fertilization., PMID:30170889
WBP2 shares a common location in mouse spermatozoa with WBP2NL/PAWP and like its descendent is a candidate mouse oocyte-activating factor., PMID:30010725
Antigen unmasking enhances visualization efficacy of the oocyte activation factor, phospholipase C zeta, in mammalian sperm., PMID:27932551
Phospholipase C-zeta deficiency as a cause for repetitive oocyte fertilization failure during ovarian stimulation for in vitro fertilization with ICSI: a case report., PMID:26174123
Variance in total levels of phospholipase C zeta (PLC-ζ) in human sperm may limit the applicability of quantitative immunofluorescent analysis as a diagnostic indicator of oocyte activation capability., PMID:23040527
Recombinant human phospholipase C zeta 1 induces intracellular calcium oscillations and oocyte activation in mouse and human oocytes., PMID:22456923
Loss of activity mutations in phospholipase C zeta (PLCζ) abolishes calcium oscillatory ability of human recombinant protein in mouse oocytes., PMID:22010140
NYD-SP27, a novel intrinsic decapacitation factor in sperm., PMID:19252507
The pattern of localization of the putative oocyte activation factor, phospholipase Czeta, in uncapacitated, capacitated, and ionophore-treated human spermatozoa., PMID:18653671
Release of phospholipase C zetaand [Ca2+]i oscillation-inducing activity during mammalian fertilization., PMID:17965260
Cell cycle-dependent Ca2+ oscillations in mouse embryos are regulated by nuclear targeting of PLCzeta., PMID:15159452
For research use only. Not for human or drug use.
