Overview
Catalog No.
PHJ14001
Species reactivity
Human
Host species
Rabbit
Isotype
IgG
Clonality
Polyclonal
Immunogen
E. coli - derived recombinant Human GDF7 (Thr322-Arg450).
Tested applications
ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000
Target
Growth/differentiation factor 7, GDF-7, GDF7
Purification
Purified by antigen affinity column.
Accession
Q7Z4P5
Applications
ELISA, IHC, WB
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Data Image
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Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with GDF7 antibody (PHJ14001) at 1 μg/ml.
Lane 1: Recombinant protein
Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.
Predict MW: 16 kDa
Observed MW: 16 kDa
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Various lysates were subjected to SDS PAGE followed by western blot with GDF7 antibody (PHJ14001) at 1 μg/ml.
Lane 1: RT-4
Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.
Predict MW: 47 kDa
Observed MW: 47 kDa
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Immunohistochemical analysis of human colon cancer tissue stained for GDF7 with PHJ14001.
References
Immune and inflammation: related factor alterations as biomarkers for predicting prognosis and responsiveness to PD-1 monoclonal antibodies in cervical cancer., PMID:36171464
TET-dependent GDF7 hypomethylation impairs aqueous humor outflow and serves as a potential therapeutic target in glaucoma., PMID:33388417
Toxicogenomic analyses of the effects of BDE-47/209, TBBPA/S and TCBPA on early neural development with a human embryonic stem cell in vitro differentiation system., PMID:31326446
GDF-5/7 and bFGF activate integrin alpha2-mediated cellular migration in rabbit ligament fibroblasts., PMID:19725104
Late-emigrating neural crest cells in the roof plate are restricted to a sensory fate by GDF7., PMID:15883363
Datasheet
For research use only. Not for human or drug use.
