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Anti-EED Polyclonal Antibody

Catalog #:   PHB23801 Specific References (50) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: O75530
Overview

Catalog No.

PHB23801

Species reactivity

Human, Mouse, Rat

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human EED (Asp142-Arg441).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

Polycomb protein EED, WAIT-1, EED, hEED, WD protein associating with integrin cytoplasmic tails 1, Embryonic ectoderm development protein

Purification

Purified by antigen affinity column.

Accession

O75530

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • Western Blot
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with EED antibody (PHB23801) at 1 μg/ml.

    Lane 1: Recombinant protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 37 kDa
    Observed MW: 37 kDa
  • Western Blot
    Various lysates were subjected to SDS PAGE followed by western blot with EED antibody (PHB23801) at 1 μg/ml.

    Lane 1: Hela
    Lane 2: K562

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 50 kDa
    Observed MW: 50 kDa
  • Immunohistochemical
    Immunohistochemical analysis of human colon cancer tissue stained for EED with PHB23801.
References

Associations of poor water, sanitation, and hygiene and parasite burden with markers of environmental enteric dysfunction in preschool-age children infected with Schistosoma mansoni in Uganda., PMID:39618064

Phase I/II study of BMS-986156 with ipilimumab or nivolumab with or without stereotactic ablative radiotherapy in patients with advanced solid malignancies., PMID:39384194

Effects of Vitamin D-3 Supplementation During Pregnancy and Lactation on Maternal and Infant Biomarkers of Environmental Enteric Dysfunction, Systemic Inflammation, and Growth: A Secondary Analysis of a Randomized Controlled Trial., PMID:39278411

Protection from environmental enteric dysfunction and growth improvement in malnourished newborns by amplification of secretory IgA., PMID:38959887

Hepatitis E associated determinants and diagnostic biomarkers during pregnancy and its prenatal consequences in Multan, Punjab tertiary care setting (Pakistan)., PMID:38883386

Direct and Detailed Site-Specific Glycopeptide Characterization by Higher-Energy Electron-Activated Dissociation Tandem Mass Spectrometry., PMID:38206681

Loss of Histone H3K27 Trimethylation (H3K27me3) Expression as a Potential Diagnostic Pitfall in Sarcomatoid Carcinoma., PMID:37997394

Phase 1/2 trial of avelumab combined with utomilumab (4-1BB agonist), PF-04518600 (OX40 agonist), or radiotherapy in patients with advanced gynecologic malignancies., PMID:37864520

Assessment of specificity of dermatopathologic criteria for IgG4-related skin disease., PMID:37853944

Epigenetically upregulating TROP2 and SLFN11 enhances therapeutic efficacy of TROP2 antibody drug conjugate sacitizumab govitecan., PMID:37567892

Tozorakimab (MEDI3506): an anti-IL-33 antibody that inhibits IL-33 signalling via ST2 and RAGE/EGFR to reduce inflammation and epithelial dysfunction., PMID:37330528

Assessing environmental enteric dysfunction via multiplex assay and its relation to growth and development among HIV-exposed uninfected Tanzanian infants., PMID:36943785

Seroprevalence of IgM and IgG Against SARS-CoV-2 after Two Doses of Pfizer-BioNTech COVID-19 Vaccine in Women with Breast Cancer., PMID:36378000

Biomarkers of environmental enteric dysfunction and adverse birth outcomes: An observational study among pregnant women living with HIV in Tanzania., PMID:36130475

Putative Biomarkers of Environmental Enteric Disease Fail to Correlate in a Cross-Sectional Study in Two Study Sites in Sub-Saharan Africa., PMID:36014817

Electron Impact Excitation of Ions from Organics on Singly Protonated Peptides with and without Post-Translational Modifications., PMID:35948044

Changes in Systemic Regulatory T Cells, Effector T Cells, and Monocyte Populations Associated With Early-Life Stunting., PMID:35720335

Association of Anti-Rotavirus IgA Seroconversion with Growth, Environmental Enteric Dysfunction and Enteropathogens in Rural Pakistani Infants., PMID:35534310

Incidence of Epstein-Barr Virus Among Women With Breast Cancer Using Monoclonal Antibodies for Latent Membrane Protein 1 (LMP1)., PMID:35478129

A Randomized Controlled Trial of Dietary Rice Bran Intake on Microbiota Diversity, Enteric Dysfunction, and Fecal Secretory IgA in Malian and Nicaraguan Infants., PMID:35441218

Impact of maternal antibodies and microbiota development on the immunogenicity of oral rotavirus vaccine in African, Indian, and European infants., PMID:34911947

An affinity-matured human monoclonal antibody targeting fusion loop epitope of dengue virus with in vivo therapeutic potency., PMID:34155267

Functional role of Tet-mediated RNA hydroxymethylcytosine in mouse ES cells and during differentiation., PMID:33009383

Dual targeting of EZH2 and androgen receptor as a novel therapy for castration-resistant prostate cancer., PMID:32805266

A prominent environmental endocrine disruptor, 4-nonylphenol, promotes endometriosis development via plasmacytoid dendritic cells., PMID:32497202

Markers of Environmental Enteric Dysfunction Are Associated with Poor Growth and Iron Status in Rural Ugandan Infants., PMID:32455424

Child Salivary SIgA and Its Relationship to Enteric Infections and EED Biomarkers in Maputo, Mozambique., PMID:32349313

Erythema elevatum et diutinum as a systemic disease., PMID:31864448

Immunostaining of Increased Expression of Enhancer of Zeste Homolog 2 (EZH2) in Diffuse Midline Glioma H3K27M-Mutant Patients with Poor Survival., PMID:31096221

Markers of Systemic Inflammation and Environmental Enteric Dysfunction Are Not Reduced by Zinc or Multivitamins in Tanzanian Infants: A Randomized, Placebo-Controlled Trial., PMID:30952509

Use of adjuvanted trivalent influenza vaccine in older-age adults: a systematic review of economic evidence., PMID:30735465

Biomarkers of maternal environmental enteric dysfunction are associated with shorter gestation and reduced length in newborn infants in Uganda., PMID:30247538

Erythema elevatum diutinum: a case report and review of literature., PMID:30074624

Data on the TGFβ response of CD4+ T cells in the absence of Eed., PMID:29876477

A targeted immunotherapy approach for HER2/neu transformed tumors by coupling an engineered effector domain with interferon-γ., PMID:29632709

Markers of Environmental Enteric Dysfunction Are Associated With Neurodevelopmental Outcomes in Tanzanian Children., PMID:29613921

Serum anti-flagellin and anti-lipopolysaccharide immunoglobulins as predictors of linear growth faltering in Pakistani infants at risk for environmental enteric dysfunction., PMID:29509790

Environmental enteric dysfunction and systemic inflammation predict reduced weight but not length gain in rural Bangladeshi children., PMID:29498344

Novel Therapy for Glioblastoma Multiforme by Restoring LRRC4 in Tumor Cells: LRRC4 Inhibits Tumor-Infitrating Regulatory T Cells by Cytokine and Programmed Cell Death 1-Containing Exosomes., PMID:29312296

Immunogenicity of rotavirus vaccine (RotarixTM) in infants with environmental enteric dysfunction., PMID:29281659

Vesiculobullous variant of erythema elevatum diutinum., PMID:28639709

Granuloma Faciale and Erythema Elevatum Diutinum in Relation to Immunoglobulin G4-Related Disease: An Appraisal of 32 Cases., PMID:27124923

Elevations in serum anti-flagellin and anti-LPS Igs are related to growth faltering in young Tanzanian children., PMID:27121948

Extended interval dosing of natalizumab in multiple sclerosis., PMID:26917698

Loss of H3K27me3 Expression Is a Highly Sensitive Marker for Sporadic and Radiation-induced MPNST., PMID:26645727

A case of mistaken identity: unilateral erythema elevatum diutinum associated with IgA paraproteinaemia., PMID:25754401

Association of serum cytokines levels, interleukin 10 -1082G/A and interferon-γ +874T/A polymorphisms with atopic asthma children from Saudi Arabia., PMID:24709010

The central role of EED in the orchestration of polycomb group complexes., PMID:24457600

Early activation of nSMase2/ceramide pathway in astrocytes is involved in ischemia-associated neuronal damage via inflammation in rat hippocampi., PMID:24007266

Monoclonal antibody for reducing the risk of respiratory syncytial virus infection in children., PMID:23633336

Datasheet

Document Download

Anti-EED Polyclonal Antibody.pdf

 

$ 170
Product specifications
50 μg 170 100 μg 280

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Anti-EED Polyclonal Antibody [PHB23801]
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