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Anti-SOCS3 Polyclonal Antibody (PHA21801)

Host species:Rabbit
Isotype:IgG
Applications:ELISA, IHC, WB
Accession:O14543
Overview

Catalog No.

PHA21801

Species reactivity

Human, Mouse, Rat

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human SOCS3 (Met1-Leu225).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

Cytokine-inducible SH2 protein 3, CIS-3, SSI-3, Suppressor of cytokine signaling 3, CIS3, STAT-induced STAT inhibitor 3, SSI3, SOCS3, SOCS-3

Purification

Purified by antigen affinity column.

Accession

O14543

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • Western Blot
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with SOCS3 antibody (PHA21801) at 1 μg/ml.

    Lane 1: Recombinant protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 27 kDa
    Observed MW: 27 kDa
  • Western Blot
    Various lysates were subjected to SDS PAGE followed by western blot with SOCS3 antibody (PHA21801) at 1 μg/ml.

    Lane 1: MOLT4
    Lane 2: A431
    Lane 3: MCF7
    Lane 4: Raw264.7
    Lane 5: H9C2

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 25 kDa
    Observed MW: 26 kDa
  • Immunohistochemical
    Immunohistochemical analysis of human colon stained for SOCS3 with PHA21801.
References
Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

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Distributor list

For research use only. Not for human or drug use.

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Anti-SOCS3 Polyclonal Antibody [PHA21801]
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