Catalog No.
PHD31301
Species reactivity
Human, Mouse, Rat
Host species
Rabbit
Isotype
IgG
Clonality
Polyclonal
Immunogen
E. coli - derived recombinant Human LIG1 (Gly484-Ile663).
Tested applications
ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000
Target
DNA ligase 1, Polydeoxyribonucleotide synthase [ATP] 1, DNA ligase I, LIG1
Purification
Purified by antigen affinity column.
Accession
P18858
Applications
ELISA, IHC, WB
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Rare TACI Mutation in a 3-Year-Old Boy With CVID Phenotype., PMID:31681716
Biallelic mutations in DNA ligase 1 underlie a spectrum of immune deficiencies., PMID:30395541
Role of apolipoprotein B100 and oxidized low-density lipoprotein in the monocyte tissue factor induction mediated by anti-β2 glycoprotein I antibodies., PMID:26964561
DNA Ligases I and III Support Nucleotide Excision Repair in DT40 Cells with Similar Efficiency., PMID:26131740
A novel method for monitoring functional lesion-specific recruitment of repair proteins in live cells., PMID:25879709
The impact of ovarian stimulation on the expression of candidate reprogramming genes in mouse preimplantation embryos., PMID:23154201
Partial complementation of a DNA ligase I deficiency by DNA ligase III and its impact on cell survival and telomere stability in mammalian cells., PMID:22460582
Arabidopsis ARP endonuclease functions in a branched base excision DNA repair pathway completed by LIG1., PMID:21781197
Inter-individual variation in DNA double-strand break repair in human fibroblasts before and after exposure to low doses of ionizing radiation., PMID:19896956
DNA ligase I and Nbs1 proteins associate in a complex and colocalize at replication factories., PMID:19597347
A novel affinity ligand for polystyrene surface from a phage display random library and its application in anti-HIV-1 ELISA system., PMID:19056299
Human DNA ligases I and III, but not ligase IV, are required for microhomology-mediated end joining of DNA double-strand breaks., PMID:18440984
Base excision repair proteins are required for integrin-mediated suppression of bleomycin-induced DNA breakage in murine lung endothelial cells., PMID:17202402
A new monoclonal antibody against DNA ligase I is a suitable marker of cell proliferation in cultured cell and tissue section samples., PMID:16377576
Distinct pools of proliferating cell nuclear antigen associated to DNA replication sites interact with the p125 subunit of DNA polymerase delta or DNA ligase I., PMID:14729473
LRIG1 protein in human cells and tissues., PMID:12684867
Homogenous expression of killer cell immunoglobulin-like receptors (KIR) on polyclonal natural killer cells detected by a monoclonal antibody to KIR2D., PMID:11034560
The replication factory targeting sequence/PCNA-binding site is required in G(1) to control the phosphorylation status of DNA ligase I., PMID:10523317
Cell-free V(D)J recombination., PMID:9242409
Reconstitution of human base excision repair with purified proteins., PMID:9200707
Specific interaction of DNA polymerase beta and DNA ligase I in a multiprotein base excision repair complex from bovine testis., PMID:8663274
Characterization of human DNA ligase I expressed in a baculovirus-insect cell system., PMID:8526875
Normal V(D)J coding junction formation in DNA ligase I deficiency syndromes., PMID:8254190
Further purification and characterization of a multienzyme complex for DNA synthesis in human cells., PMID:8300757
Purification and characterization of DNA ligase I from the trypanosomatid Crithidia fasciculata., PMID:1508676
Mammalian DNA ligases. Catalytic domain and size of DNA ligase I., PMID:1695631
DNA ligases from rat liver. Purification and partial characterization of two molecular forms., PMID:2383569
Defective DNA ligase I in Bloom's syndrome cells. Simultaneous analysis using immunoblotting and the ligase-[32P]AMP adduct assay., PMID:3192533
For research use only. Not for human or drug use.
