Catalog No.
PHA29901
Species reactivity
Human, Mouse, Rat
Host species
Rabbit
Isotype
IgG
Clonality
Polyclonal
Immunogen
E. coli - derived recombinant Human NDC80/HEC1 (Pro290-Glu642).
Tested applications
ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000
Target
HEC1, Kinetochore protein Hec1, HsHec1, HEC, Kinetochore protein NDC80 homolog, Kinetochore-associated protein 2, NDC80, Highly expressed in cancer protein, Retinoblastoma-associated protein HEC, KNTC2
Purification
Purified by antigen affinity column.
Accession
O14777
Applications
ELISA, IHC, WB
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
KNL1 and NDC80 represent new universal markers for the detection of functional centromeres in plants., PMID:38403686
A TCR mimic CAR T cell specific for NDC80 is broadly reactive with solid tumors and hematologic malignancies., PMID:35427421
Splicing factors Sf3A2 and Prp31 have direct roles in mitotic chromosome segregation., PMID:30475206
A quantitative description of Ndc80 complex linkage to human kinetochores., PMID:26345214
Protein purification technique that allows detection of sumoylation and ubiquitination of budding yeast kinetochore proteins Ndc10 and Ndc80., PMID:25992961
Distinct organization and regulation of the outer kinetochore KMN network downstream of CENP-C and CENP-T., PMID:25660545
Phosphorylation of centromeric histone H3 variant regulates chromosome segregation in Saccharomyces cerevisiae., PMID:23637466
Chk1 and Mps1 jointly regulate correction of merotelic kinetochore attachments., PMID:23321637
The Ndc80 internal loop is required for recruitment of the Ska complex to establish end-on microtubule attachment to kinetochores., PMID:22454517
Ndc80 regulates meiotic spindle organization, chromosome alignment, and cell cycle progression in mouse oocytes., PMID:21600073
Direct binding of Cenp-C to the Mis12 complex joins the inner and outer kinetochore., PMID:21353556
CENP-C is a structural platform for kinetochore assembly., PMID:21353555
Relative contributions of chromatin and kinetochores to mitotic spindle assembly., PMID:19805628
Hec1 contributes to mitotic centrosomal microtubule growth for proper spindle assembly through interaction with Hice1., PMID:19776357
Chromosome congression in the absence of kinetochore fibres., PMID:19525938
The mitotic checkpoint kinase NEK2A regulates kinetochore microtubule attachment stability., PMID:18297113
Kinetochore microtubule dynamics and attachment stability are regulated by Hec1., PMID:17129782
Hec1 sequentially recruits Zwint-1 and ZW10 to kinetochores for faithful chromosome segregation and spindle checkpoint control., PMID:16732327
Polo-like kinase 1 creates the tension-sensing 3F3/2 phosphoepitope and modulates the association of spindle-checkpoint proteins at kinetochores., PMID:15964272
Molecular organization of the Ndc80 complex, an essential kinetochore component., PMID:15809444
Hec1 and nuf2 are core components of the kinetochore outer plate essential for organizing microtubule attachment sites., PMID:15548592
Kinetochore localization and microtubule interaction of the human spindle checkpoint kinase Mps1., PMID:15235793
The vertebrate Ndc80 complex contains Spc24 and Spc25 homologs, which are required to establish and maintain kinetochore-microtubule attachment., PMID:14738735
Nuf2 and Hec1 are required for retention of the checkpoint proteins Mad1 and Mad2 to kinetochores., PMID:14654001
The highly conserved Ndc80 complex is required for kinetochore assembly, chromosome congression, and spindle checkpoint activity., PMID:12514103
Phosphorylation of the mitotic regulator protein Hec1 by Nek2 kinase is essential for faithful chromosome segregation., PMID:12386167
HEC, a novel nuclear protein rich in leucine heptad repeats specifically involved in mitosis., PMID:9315664
For research use only. Not for human or drug use.
