Catalog No.
PHG93801
Species reactivity
Human
Host species
Rabbit
Isotype
IgG
Clonality
Polyclonal
Immunogen
E. coli - derived recombinant Human NUMA1/NMP-22 (Met1-Lys153).
Tested applications
ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000
Target
SP-H antigen, NMP-22, Nuclear matrix protein-22, NMP22, NuMA protein, Nuclear mitotic apparatus protein, NUMA, Nuclear mitotic apparatus protein 1, NUMA1
Purification
Purified by antigen affinity column.
Accession
Q14980
Applications
ELISA, IHC, WB
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Golimumab-Induced Anti-NuMA-1 (Nuclear Mitotic Apparatus Protein 1) Antibodies in a Rheumatoid Arthritis Patient: A Case Report., PMID:39628740
Anti-NuMA antibodies: clinical associations and significance in patients with primary Sjögren's syndrome or systemic lupus erythematosus., PMID:33404653
Establishment of international autoantibody reference standards for the detection of autoantibodies directed against PML bodies, GW bodies, and NuMA protein., PMID:32776893
Global Interactomics Connect Nuclear Mitotic Apparatus Protein NUMA1 to Influenza Virus Maturation., PMID:30572664
Electrochemical ELISA-based platform for bladder cancer protein biomarker detection in urine., PMID:30005382
NuMA phosphorylation by CDK1 couples mitotic progression with cortical dynein function., PMID:23921553
Rad53 homologue forkhead-associated kinase A (FhkA) and Ca2+-binding protein 4a (CBP4a) are nucleolar proteins that differentially redistribute during mitosis in Dictyostelium., PMID:23587254
Autoantibodies to 60kDa SS-A/Ro yield a specific nuclear myriad discrete fine speckled immunofluorescence pattern., PMID:23357050
Assessment of autoantibodies to meningioma in a population-based study., PMID:23221727
Influencing factors on the NMP-22 urine assay: an experimental model., PMID:22928931
Survival and differentiation of human embryonic stem cell-derived neural precursors grafted spinally in spinal ischemia-injured rats or in naive immunosuppressed minipigs: a qualitative and quantitative study., PMID:22889456
GAS41 amplification results in overexpression of a new spindle pole protein., PMID:22619067
Poly(ADP-ribose) polymerase 3 (PARP3), a newcomer in cellular response to DNA damage and mitotic progression., PMID:21270334
Robust control of mitotic spindle orientation in the developing epidermis., PMID:21098114
Anti-NuMA1 and anti-NuMA2 (anti-HsEg5) antibodies: Clinical and immunological features: A propos of 40 new cases and review of the literature., PMID:20457279
Defining the molecular target of an antibody derived from nuclear extract of Jurkat cells using protein arrays., PMID:19733143
Redefining the Scl-70 indirect immunofluorescence pattern: autoantibodies to DNA topoisomerase I yield a specific compound immunofluorescence pattern., PMID:19395540
Identification and characterization of INMAP, a novel interphase nucleus and mitotic apparatus protein that is involved in spindle formation and cell cycle progression., PMID:19331820
Cohesin subunit SMC1 associates with mitotic microtubules at the spindle pole., PMID:18832153
The spindle function of CDCA4., PMID:18498124
Antibodies to mitotic spindle apparatus: clinical significance of NuMA and HsEg5 autoantibodies., PMID:18240009
The control of tissue architecture over nuclear organization is crucial for epithelial cell fate., PMID:17405811
Inactivation of MAPK affects centrosome assembly, but not actin filament assembly, in mouse oocytes maturing in vitro., PMID:17219430
NuMA influences higher order chromatin organization in human mammary epithelium., PMID:17108325
Autoantibodies to mitotic apparatus: association with other autoantibodies and their clinical significance., PMID:16941236
The Nuclear Mitotic Apparatus (NuMA) protein is contributed by the donor cell nucleus in cloned porcine embryos., PMID:16368570
Dynamic changes in NuMA and microtubules in monkey-rabbit nuclear transfer embryos., PMID:16368565
Caspase-3 is required in the apoptotic disintegration of the nuclear matrix., PMID:16199031
Function of donor cell centrosome in intraspecies and interspecies nuclear transfer embryos., PMID:15878330
Multiple mechanisms regulate NuMA dynamics at spindle poles., PMID:15561764
XRHAMM functions in ran-dependent microtubule nucleation and pole formation during anastral spindle assembly., PMID:15498487
NuMA expression and function in mouse oocytes and early embryos., PMID:15067221
XMAP215, XKCM1, NuMA, and cytoplasmic dynein are required for the assembly and organization of the transient microtubule array during the maturation of Xenopus oocytes., PMID:14499655
[Clinical significance of fluoroscopic patterns specific for the mitotic spindle in patients with rheumatic diseases]., PMID:12404031
Unraveling the organization of the internal nuclear matrix: RNA-dependent anchoring of NuMA to a lamin scaffold., PMID:12243746
Pim-1 associates with protein complexes necessary for mitosis., PMID:12111331
A mammalian Partner of inscuteable binds NuMA and regulates mitotic spindle organization., PMID:11781568
Further considerations on the intranuclear distribution of HMGI/Y proteins., PMID:11767201
In vitro induction and in vivo expression of bcl-2 in the hNT neurons., PMID:11704352
A potential role for human cohesin in mitotic spindle aster assembly., PMID:11590136
Immunolocalization of NuMA and phosphorylated proteins during the cell cycle in human breast and prostate cancer cells as analyzed by immunofluorescence and postembedding immunoelectron microscopy., PMID:11449886
Gender-dependent specific immune response during chronic human Schistosomiasis haematobia., PMID:11359443
The effects of taurine on hNT neurons transplanted in adult rat striatum., PMID:11202562
Chromosome movement in mitosis requires microtubule anchorage at spindle poles., PMID:11157972
GAS41, a highly conserved protein in eukaryotic nuclei, binds to NuMA., PMID:10913114
Spindle formation and dynamics of gamma-tubulin and nuclear mitotic apparatus protein distribution during meiosis in pig and mouse oocytes., PMID:10775165
Chromosomal instability and cytoskeletal defects in oral cancer cells., PMID:10618413
Ran-GTP stabilises microtubule asters and inhibits nuclear assembly in Xenopus egg extracts., PMID:10381400
Influence of different metal ions on the ultrastructure, biochemical properties, and protein localization of the K562 cell nuclear matrix., PMID:10321834
The plant nucleoskeleton: ultrastructural organization and identification of NuMA homologues in the nuclear matrix and mitotic spindle of plant cells., PMID:9925768
For research use only. Not for human or drug use.
