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Anti-IDS Polyclonal Antibody

Catalog #:   PHD50901 Specific References (50) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: P22304
Overview

Catalog No.

PHD50901

Species reactivity

Human, Mouse, Rat

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human IDS (Arg95-Pro289).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

Alpha-L-iduronate sulfate sulfatase,Iduronate 2-sulfatase,SIDS,Idursulfase,IDS

Purification

Purified by antigen affinity column.

Accession

P22304

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • Western blot
    Various lysates were subjected to SDS PAGE followed by western blot with IDS antibody (PHD50901) at 0.35ug/ml.

    Lane 1: NCI-H460 cell lysate
    Lane 2: 293T cell lysate

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 61,34 kDa
    Observed MW: 68,35 kDa
  • Immunocytochemistry/ Immunofluorescence
    IDS in Hela Cell Line.
    The Hela cells were fixed with 4% paraformaldehyde (20 min), and then blocked with 5% goat serum for 1h. And the cells were incubated for 2h at 37°C with IDS (PHD50901) at 3.5 μg/ml. The section was then incubated with Goat Anti-Rabbit IgG (Alexa Fluor-594) preabsorbed at 1/100 dilution (Shown in red) for 1 hour at room temperature.Nuclear DNA was labelled with DAPI (shown in blue).
  • Western Blot
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with IDS antibody (PHD50901) at 1 µg/ml.

    Lane 1: Recombinant Protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 25 kDa
    Observed MW: 25 kDa
References

Parameter-Optimized Fabrication of Submicron Nanoelectrospray Emitters for Enhanced Native Mass Spectrometry., PMID:40456619

Understanding Heritable Variation Among Hosts in Infectious Diseases Through the Lens of Twin Studies., PMID:40004506

Anti-Idiotypic Antibody as a Booster Vaccine Against Respiratory Syncytial Virus., PMID:39852814

In vivo brain delivery of BBB-enabled iduronate 2-sulfatase in rats., PMID:39810248

Construction of in situ modulated controlled growth of MOF-on-mof impedimetric assembly for the practical minimal level assessment of anti-mullerian hormone., PMID:39754847

Trajectory of beta cell function and insulin clearance in stage 2 type 1 diabetes: natural history and response to teplizumab., PMID:39560746

Ultrasensitive aflatoxin B1 detection based on vertical organic electrochemical transistor., PMID:39423541

A Programme of Hepatitis C Surveillance With Active Linkage to Care (HEAL) for Inpatients in Two Tertiary Hospitals in Jiangsu, China., PMID:39382123

Prognostic analysis of peritoneal washing cytology during interval debulking surgery in advanced ovarian cancer., PMID:39182152

In Vivo Proximity Cross-Linking and Immunoprecipitation of Cell Wall Epitopes Identify Proteins Associated with the Biosynthesis of Matrix Polysaccharides., PMID:39072051

Novel approaches to enable equitable access to monoclonal antibodies in low- and middle-income countries., PMID:38950021

Phase I and Randomized Phase II Study of Ruxolitinib With Frontline Neoadjuvant Therapy in Advanced Ovarian Cancer: An NRG Oncology Group Study., PMID:38776484

Emerging treatments in HER2-positive advanced breast cancer: Keep raising the bar., PMID:38759648

Paracrinal regulation of neutrophil functions by coronaviral infection in iPSC-derived alveolar type II epithelial cells., PMID:38754785

Selection of positive controls and their impact on anti-drug antibody assay performance., PMID:38479453

Clinical characteristics and treatment modalities in women with newly diagnosed advanced high-grade serous epithelial ovarian cancer in Taiwan., PMID:38453530

Analytical and diagnostic performance of Theradiag i-Tracker assays on IDS-iSYS for infliximab and adalimumab therapeutic drug monitoring., PMID:38379379

Screening Non-neutralizing Anti-idiotype Antibodies Against a Drug Candidate for Total Pharmacokinetic and Target Engagement Assay., PMID:38267774

A close-up view of the Hunter syndrome., PMID:38241811

Automated Bioanalytical Workflow for Ligand Binding-Based Pharmacokinetic Assay Development., PMID:38156369

Associations of Changes in Bone Turnover Markers with Change in Bone Mineral Density in Kidney Transplant Patients., PMID:38030558

The impact of erythropoiesis-stimulating agents administration concomitantly with adjuvant anti-HER2 treatments on the outcomes of patients with early breast cancer: a sub-analysis of the ALTTO study., PMID:37938495

Trends in prevalence, treatment use, and disease burden in patients with eosinophilic granulomatosis with polyangiitis in Japan: Real-world database analysis., PMID:37930840

Immunological and clinicopathological features predict HER2-positive breast cancer prognosis in the neoadjuvant NeoALTTO and CALGB 40601 randomized trials., PMID:37923752

Paclitaxel plus carboplatin and durvalumab with or without oleclumab for women with previously untreated locally advanced or metastatic triple-negative breast cancer: the randomized SYNERGY phase I/II trial., PMID:37919269

Transferrin Receptor-Targeted Iduronate-2-sulfatase Penetrates the Blood-Retinal Barrier and Improves Retinopathy in Mucopolysaccharidosis II Mice., PMID:37860991

Real-world clinical outcomes of patients with stage I HER2-positive breast cancer treated with adjuvant paclitaxel and trastuzumab., PMID:37562696

Modeling the kinetics of the neutralizing antibody response against SARS-CoV-2 variants after several administrations of Bnt162b2., PMID:37549192

Phage-derived anti-idiotype and anti-YTE antibodies in development of MK-1654 pharmacokinetic and immune response assays., PMID:37515532

Ten-year safety and clinical benefit from open-label etanercept treatment in children and young adults with juvenile idiopathic arthritis., PMID:37140539

Real-world study of bevacizumab treatment in patients with ovarian cancer: a Chinese single-institution study of 155 patients., PMID:37055754

Immunoinformatics Study: Multi-Epitope Based Vaccine Design from SARS-CoV-2 Spike Glycoprotein., PMID:36851275

Immunotherapy in breast cancer: an overview of current strategies and perspectives., PMID:36781869

SpikeScape: A Tool for Analyzing Structural Diversity in Experimental Structures of the SARS-CoV-2 Spike Glycoprotein., PMID:36758040

The Heterogeneity of Ovomucoid-Specific IgE Idiotype Is Associated With Egg Allergy Symptom Severity., PMID:36693362

Cardiac safety of dual anti-HER2 blockade with pertuzumab plus trastuzumab in early HER2-positive breast cancer in the APHINITY trial., PMID:36681013

Use of durvalumab in stage III non-small-cell lung cancer based on eligibility for the PACIFIC study., PMID:36627112

A New Laboratory Workflow Integrating the Free Light Chains Kappa Quotient into Routine CSF Analysis., PMID:36421703

Outcomes of patients with small and node-negative HER2-positive early breast cancer treated with adjuvant chemotherapy and anti-HER2 therapy-a sub-analysis of the ALTTO study., PMID:36050448

Immune response to anti-SARS-CoV-2 prime-vaccination in patients with cancer: a systematic review and meta-analysis., PMID:35867203

Selective quantification of the 22-kDa isoform of human growth hormone 1 in serum and plasma by immunocapture and LC-MS/MS., PMID:35838770

Real-life data on treatment and outcomes in advanced ovarian cancer: An observational, multinational cohort study (RESPONSE trial)., PMID:35714310

Standardisation of ACPA tests: evaluation of a new candidate reference preparation., PMID:35697487

Sacituzumab govitecan as second-line treatment for metastatic triple-negative breast cancer-phase 3 ASCENT study subanalysis., PMID:35680967

Effect of Anti-Iduronate 2-Sulfatase Antibodies in Patients with Mucopolysaccharidosis Type II Treated with Enzyme Replacement Therapy., PMID:35568060

Exploration of quantitative site-specific serum O-glycoproteomics with isobaric labeling for the discovery of putative O-glycoprotein biomarkers., PMID:35507764

Screening and identification of vancomycin anti-idiotypic antibodies for against Staphylococcus aureus from a human phage display domain antibody library., PMID:35504507

Impact of an anti-infective screening and monitoring protocol together with infectious disease consultation in preventing infective adverse events in patients treated with anti-CD20/CD52 agents for multiple sclerosis., PMID:35487032

Six-year absolute invasive disease-free survival benefit of adding adjuvant pertuzumab to trastuzumab and chemotherapy for patients with early HER2-positive breast cancer: A Subpopulation Treatment Effect Pattern Plot (STEPP) analysis of the APHINITY (BIG 4-11) trial., PMID:35313167

Molecular architecture determines brain delivery of a transferrin receptor-targeted lysosomal enzyme., PMID:35226042

Datasheet

Document Download

Anti-IDS Polyclonal Antibody.pdf

 

$ 170
Product specifications
50 μg 170 100 μg 280

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Anti-IDS Polyclonal Antibody [PHD50901]
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