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Anti-CD9 Polyclonal Antibody (PHD48801)

Host species:Rabbit
Isotype:IgG
Applications:ELISA, IHC, WB
Accession:P21926
Overview

Catalog No.

PHD48801

Species reactivity

Human, Mouse, Rat

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human CD9 (Ser112-Ile195).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

Motility-related protein,Leukocyte antigen MIC3,TSPAN29,Cell growth-inhibiting gene 2 protein,CD9,MIC3,p24,Tspan-29,MRP-1,5H9 antigen,Tetraspanin-29,CD9 antigen

Concentration

2.16 mg/ml

Purification

Purified by antigen affinity column.

Accession

P21926

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • WESTERN BLOT
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with CD9 antibody (PHD48801) at 1 μg/ml.

    Lane 1: Recombinant protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 37 kDa
    Observed MW: 37 kDa
References

Automated On-Line Isolation and Fractionation System for Nanosized Biomacromolecules from Human Plasma. PMID: 32893620

Regulation of cancer stem cell properties by CD9 in human B-acute lymphoblastic leukemia. PMID: 21549094

CD9-dependent regulation of Canine distemper virus-induced cell-cell fusion segregates with the extracellular domain of the haemagglutinin. PMID: 16690928

An immunoassay for urinary extracellular vesicles. PMID: 26823283

Functional association of CD9 with the Fc gamma receptors in macrophages. PMID: 11207280

Tetraspanin family protein CD9 in the mouse sperm: unique localization, appearance, behavior and fate during fertilization. PMID: 20428892

Localization of CD9 in pig oocytes and its effects on sperm-egg interaction. PMID: 15056780

CD9-mediated costimulation of TCR-triggered naive T cells leads to activation followed by apoptosis. PMID: 9378967

Differential activation of human platelets induced by Fc gamma receptor II cross-linking and by anti-CD9 monoclonal antibody. PMID: 8955216

Isolation of prostate cancer-related exosomes. PMID: 24982349

CD9 is associated with leukemia inhibitory factor-mediated maintenance of embryonic stem cells. PMID: 11950938

CD9 controls the formation of clusters that contain tetraspanins and the integrin alpha 6 beta 1, which are involved in human and mouse gamete fusion. PMID: 16418227

Anti-GPIIb/IIIa (CD41) monoclonal antibody-induced platelet activation requires Fc receptor-dependent cell-cell interaction. PMID: 1832937

[Inhibition of the motility of melanoma cells using interference RNA against CD9]. PMID: 16476329

Apoptosis signal-regulating kinase 1 regulates immune-mediated thrombocytopenia, thrombosis, and systemic shock. PMID: 32767736

Expression of CD9 antigen on normal activated human B cells. PMID: 2673548

Prognostic significance of CD9 expression in locally advanced gastric cancer treated with surgery and adjuvant chemoradiotherapy. PMID: 20547009

Contrasting effects of EWI proteins, integrins, and protein palmitoylation on cell surface CD9 organization. PMID: 16537545

Stromal cell CD9 regulates differentiation of hematopoietic stem/progenitor cells. PMID: 10194438

The tetraspanin CD9 inhibits the proliferation and tumorigenicity of human colon carcinoma cells. PMID: 17582603

Use of CD9 and CD61 for the characterization of AML-M7 by flow cytometry in a dog*. PMID: 22235968

Monoclonal antibody to CD9 inhibits platelet-induced human endothelial cell proliferation. PMID: 16951553

Role of transmembrane 4 superfamily (TM4SF) proteins CD9 and CD81 in muscle cell fusion and myotube maintenance. PMID: 10459022

CD9-mediated activation of the p46 Shc isoform leads to apoptosis in cancer cells. PMID: 15226408

The tetraspanin CD9 regulates migration, adhesion, and homing of human cord blood CD34+ hematopoietic stem and progenitor cells. PMID: 21063023

CD9 is a surface marker on mouse and rat male germline stem cells. PMID: 12954725

Analysis of the tetraspanin CD9-integrin alphaIIbbeta3 (GPIIb-IIIa) complex in platelet membranes and transfected cells. PMID: 9355765

Identification of CD9 extracellular domains important in regulation of CHO cell adhesion to fibronectin and fibronectin pericellular matrix assembly. PMID: 12453879

Regulatory effect of CD9 on calcium-stimulated phosphatidylserine exposure in Jurkat T lymphocytes. PMID: 9500845

Datasheet

Document Download

Anti-CD9 Polyclonal Antibody.pdf

 

$ 170
Product specifications
50 μg 170 100 μg 280

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For research use only. Not for human or drug use.

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Anti-CD9 Polyclonal Antibody [PHD48801]
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