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Anti-CD9 Polyclonal Antibody

Catalog #:   PHD48801 Specific References (79) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: P21926
Overview

Catalog No.

PHD48801

Species reactivity

Human, Mouse, Rat

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human CD9 (Ser112-Ile195).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

Motility-related protein,Leukocyte antigen MIC3,TSPAN29,Cell growth-inhibiting gene 2 protein,CD9,MIC3,p24,Tspan-29,MRP-1,5H9 antigen,Tetraspanin-29,CD9 antigen

Concentration

2.16 mg/ml

Purification

Purified by antigen affinity column.

Accession

P21926

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • WESTERN BLOT
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with CD9 antibody (PHD48801) at 1 μg/ml.

    Lane 1: Recombinant protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 37 kDa
    Observed MW: 37 kDa
References

Automated On-Line Isolation and Fractionation System for Nanosized Biomacromolecules from Human Plasma. PMID: 32893620

Regulation of cancer stem cell properties by CD9 in human B-acute lymphoblastic leukemia. PMID: 21549094

CD9-dependent regulation of Canine distemper virus-induced cell-cell fusion segregates with the extracellular domain of the haemagglutinin. PMID: 16690928

An immunoassay for urinary extracellular vesicles. PMID: 26823283

Functional association of CD9 with the Fc gamma receptors in macrophages. PMID: 11207280

Tetraspanin family protein CD9 in the mouse sperm: unique localization, appearance, behavior and fate during fertilization. PMID: 20428892

Localization of CD9 in pig oocytes and its effects on sperm-egg interaction. PMID: 15056780

CD9-mediated costimulation of TCR-triggered naive T cells leads to activation followed by apoptosis. PMID: 9378967

Differential activation of human platelets induced by Fc gamma receptor II cross-linking and by anti-CD9 monoclonal antibody. PMID: 8955216

Isolation of prostate cancer-related exosomes. PMID: 24982349

CD9 is associated with leukemia inhibitory factor-mediated maintenance of embryonic stem cells. PMID: 11950938

CD9 controls the formation of clusters that contain tetraspanins and the integrin alpha 6 beta 1, which are involved in human and mouse gamete fusion. PMID: 16418227

Anti-GPIIb/IIIa (CD41) monoclonal antibody-induced platelet activation requires Fc receptor-dependent cell-cell interaction. PMID: 1832937

[Inhibition of the motility of melanoma cells using interference RNA against CD9]. PMID: 16476329

Apoptosis signal-regulating kinase 1 regulates immune-mediated thrombocytopenia, thrombosis, and systemic shock. PMID: 32767736

Expression of CD9 antigen on normal activated human B cells. PMID: 2673548

Prognostic significance of CD9 expression in locally advanced gastric cancer treated with surgery and adjuvant chemoradiotherapy. PMID: 20547009

Contrasting effects of EWI proteins, integrins, and protein palmitoylation on cell surface CD9 organization. PMID: 16537545

Stromal cell CD9 regulates differentiation of hematopoietic stem/progenitor cells. PMID: 10194438

The tetraspanin CD9 inhibits the proliferation and tumorigenicity of human colon carcinoma cells. PMID: 17582603

Use of CD9 and CD61 for the characterization of AML-M7 by flow cytometry in a dog*. PMID: 22235968

Monoclonal antibody to CD9 inhibits platelet-induced human endothelial cell proliferation. PMID: 16951553

Role of transmembrane 4 superfamily (TM4SF) proteins CD9 and CD81 in muscle cell fusion and myotube maintenance. PMID: 10459022

CD9-mediated activation of the p46 Shc isoform leads to apoptosis in cancer cells. PMID: 15226408

The tetraspanin CD9 regulates migration, adhesion, and homing of human cord blood CD34+ hematopoietic stem and progenitor cells. PMID: 21063023

CD9 is a surface marker on mouse and rat male germline stem cells. PMID: 12954725

Analysis of the tetraspanin CD9-integrin alphaIIbbeta3 (GPIIb-IIIa) complex in platelet membranes and transfected cells. PMID: 9355765

Identification of CD9 extracellular domains important in regulation of CHO cell adhesion to fibronectin and fibronectin pericellular matrix assembly. PMID: 12453879

Regulatory effect of CD9 on calcium-stimulated phosphatidylserine exposure in Jurkat T lymphocytes. PMID: 9500845

An Extracellular Vesicle (EV) Paper Strip for Rapid and Convenient Estimation of EV Concentration., PMID:40422033

Development of a high-sensitivity assay for direct quantitation of EGFr on extracellular vesicles in plasma., PMID:40345522

[Efficient capture and proteomics analysis of urinary extracellular vesicles by affinity purification]., PMID:40331614

Novel extracellular vesicle release pathway facilitated by toxic superoxide dismutase 1 oligomers., PMID:40291716

Hetero Sandwich Immunoassay as Tool to Probe the Composition of the Extracellular Vesicles Membranes: The Case Study of L1CAM Localization., PMID:40224478

Sensitive detection of small extracellular vesicles using a gold nanostar-based SERS assay., PMID:40202797

Microfluidic Nano-Plasmonic Imaging Platform for Purification- and Label-Free Single Small Extracellular Vesicle Counting., PMID:40166220

Next Generation Aqueous Two-Phase System for Gentle, Effective, and Timely Extracellular Vesicle Isolation and Transcriptomic Analysis., PMID:40108918

Single-cell multiomic comparison of mouse and rat spermatogenesis reveals gene regulatory networks conserved for over 20 million years., PMID:40086448

IL-16 exerts anti-rabies virus effects through CD9 on the surface of viral particles., PMID:39956243

Single-cell RNA sequencing defines distinct disease subtypes and reveals hypo-responsiveness to interferon in asymptomatic Waldenstrom's Macroglobulinemia., PMID:39929803

Mesenchymal stem cells from perinatal tissues promote diabetic wound healing via PI3K/AKT activation., PMID:39923118

EV-Lev: extracellular vesicle isolation from human plasma using microfluidic magnetic levitation device., PMID:39918033

Nanoliter Plasma Sample for Disease Diagnosis by Using a Visualization Platform of Counting Extracellular Vesicle Particles., PMID:39895388

Dual-target magneto-immunoassay with bifunctional nanohybrids for breast cancer exosome detection., PMID:39788070

Elucidating Extracellular Vesicle Isolation Kinetics via an Integrated Off-Stoichiometry Thiol-Ene and Cyclic Olefin Copolymer Microfluidic Device., PMID:39771431

The isolation of VCAM-1+ endothelial cell-derived extracellular vesicles using microfluidics., PMID:39698414

EV-Elute: A universal platform for the enrichment of functional surface marker-defined extracellular vesicle subpopulations., PMID:39692115

B cell c-Maf signaling promotes tumor progression in animal models of pancreatic cancer and melanoma., PMID:39608978

Proteomics of circulating extracellular vesicles reveals diverse clinical presentations of COVID-19 but fails to identify viral peptides., PMID:39569406

Extracellular vesicles containing SARS-CoV-2 proteins are associated with multi-organ dysfunction and worse outcomes in patients with severe COVID-19., PMID:39558820

Serum Exosomes Expressing CD9, CD63 and HER2 From Breast-Cancer Patients Decreased After Surgery of the Primary Tumor: A Potential Biomarker of Tumor Burden., PMID:39467625

Citrullination Accompanies the Development of Carotid Atherosclerotic Plaques., PMID:39421992

Comparison of nanoimaging and nanoflow based detection of extracellular vesicles at a single particle resolution., PMID:39416671

Enhancing Extracellular Vesicle Detection via Cotargeting Tetraspanin Biomarkers., PMID:39360503

Multiparametric profiling of HER2-enriched extracellular vesicles in breast cancer using Single Extracellular VEsicle Nanoscopy., PMID:39342336

Sensitive and reliable lab-on-paper biosensor for label-free detection of exosomes by electrochemical impedance spectroscopy., PMID:39316098

Spatiotemporal Delivery of Dual Nanobodies by Engineered Probiotics to Reverse Tumor Immunosuppression via Targeting Tumor-Derived Exosomes., PMID:39308426

Immobilization coupling with aptamer assisted dual cycle amplification for sensitive sEVs isolation and analysis., PMID:39266887

Sensitive phenotyping of serum extracellular vesicles on a SERS-microfluidic platform for early-stage clinical diagnosis of ovarian carcinoma., PMID:39260102

Isolation and analysis of the exosomal membrane proteins in bullous pemphigoid., PMID:39230197

Evaluation of non-invasive biomarkers of kidney allograft rejection in a prospective multicenter unselected cohort study (EU-TRAIN)., PMID:39197587

Extracellular vesicles derived from antigen-presenting cells pulsed with foot and mouth virus vaccine-antigens act as carriers of viral proteins and stimulate B cell response., PMID:39176090

Comparison of EV characterization by commercial high-sensitivity flow cytometers and a custom single-molecule flow cytometer., PMID:39140467

Urinary Extracellular Vesicles as a Readily Available Biomarker Source: A Simplified Stratification Method., PMID:39125575

Exosomal membrane proteins analysis using a silicon nanowire field effect transistor biosensor., PMID:39002259

Impact of brain organoid-derived sEVs on metastatic adaptation and invasion of breast carcinoma cells through a microphysiological system., PMID:38888211

Automatic Electrochemiluminescence Method for the Detection of Cancerous Exosomes Incorporating Specific Aptamer-Magnetic Beads and Signal Nanoprobes., PMID:38866706

Anti-pig antibodies in swine veterinarian serum: Implications for clinical xenotransplantation., PMID:38853364

Measurable Residual Disease Analysis by Flow Cytometry and Correlation With Molecular Measurable Residual Disease in Acute Promyelocytic Leukemia: A Real-World Prospective Study., PMID:38838353

Bilateral efforts to improve SERS detection efficiency of exosomes by Au/Na7PMo11O39 Combined with Phospholipid Epitope Imprinting., PMID:38705072

Deep learning insights into the architecture of the mammalian egg-sperm fusion synapse., PMID:38666763

Flow cytometric immunophenotypic differentiation patterns of bone marrow eosinophilopoiesis., PMID:38666394

Exhaled breath condensate contains extracellular vesicles (EVs) that carry miRNA cargos of lung tissue origin that can be selectively purified and analyzed., PMID:38659349

Spectroscopic insight into breast cancer: profiling small extracellular vesicles lipids via infrared spectroscopy for diagnostic precision., PMID:38654096

CyTOF analysis revealed platelet heterogeneity in breast cancer patients received T-DM1 treatment., PMID:38643891

Deciphering pancreatic neuroendocrine tumors: Unveiling through circulating small extracellular vesicles., PMID:38596136

A molecular docking exploration of the large extracellular loop of tetraspanin CD81 with small molecules., PMID:38584777

Computational prediction of protein interactions in single cells by proximity sequencing., PMID:38483861

Distinguishing two distinct types of salivary extracellular vesicles: a potential tool for understanding their pathophysiological roles., PMID:38481961

Datasheet

Document Download

Anti-CD9 Polyclonal Antibody.pdf

 

$ 170
Product specifications
50 μg 170 100 μg 280

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Anti-CD9 Polyclonal Antibody [PHD48801]
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