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Anti-DHPS Polyclonal Antibody

Catalog #:   PHE64801 Specific References (50) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: P49366
Overview

Catalog No.

PHE64801

Species reactivity

Human, Mouse, Rat

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human DHPS (Met1-Asp369).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:200, WB: 1:500-1:4000

Target

DHPS, Deoxyhypusine synthase, DHS, DS

Concentration

0.6 mg/ml

Purification

Purified by antigen affinity column.

Accession

P49366

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • Western Blot
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with DHPS antibody (PHE64801) at 1 μg/ml.

    Lane 1: Recombinant protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 43 kDa
    Observed MW: 43 kDa
  • Western Blot
    Various lysates were subjected to SDS PAGE followed by western blot with DHPS antibody (PHE64801) at 1 μg/ml.

    Lane 1: 3T3

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 41 kDa
    Observed MW: 42 kDa
  • Immunohistochemical
    Immunohistochemical analysis of mouse colorectal tissue stained for DHPS with PHE64801.
References

High-fidelity in silico generation and augmentation of TCR repertoire data using generative adversarial networks., PMID:40419551

Development of a reliable, sensitive, and convenient assay for the discovery of new eIF5A hypusination inhibitors., PMID:39937781

Novel deoxyhypusine synthase (DHPS) inhibitors target hypusination-induced vasculogenic mimicry (VM) against malignant melanoma., PMID:39393437

DHPS-Mediated Hypusination Regulates METTL3 Self-m6A-Methylation Modification to Promote Melanoma Proliferation and the Development of Novel Inhibitors., PMID:38952061

Sustained clinical benefit of malaria chemoprevention with sulfadoxine-pyrimethamine (SP) in pregnant women in a region with high SP resistance markers., PMID:38574776

Dihydropteroate synthase based sensor for screening multi-sulfonamides residue and its comparison with broad-specific antibody based immunoassay by molecular modeling analysis., PMID:30661581

Class-Specific Monoclonal Antibodies and Dihydropteroate Synthase in Bioassays Used for the Detection of Sulfonamides: Structural Insights into Recognition Diversity., PMID:30580515

Evidence that endogenous formaldehyde produces immunogenic and atherogenic adduct epitopes., PMID:28883613

A purified MAA-based ELISA is a useful tool for determining anti-MAA antibody titer with high sensitivity., PMID:28222187

A highly sensitive and class-specific fluorescence polarisation assay for sulphonamides based on dihydropteroate synthase., PMID:25775967

Isolation and characterization of Toxoplasma gondii genotypes from goats at an abattoir in Okinawa., PMID:22446126

Efficacy of the fibroblast growth factor inhibitor 2,5-dihydroxyphenylsulfonate in basal cell carcinoma: a histopathological and inmunohistochemical study., PMID:21756148

Hemoperfusion treatment in a septic shock patient with autosomal dominant polycystic kidney disease and increased HMGB1 protein levels., PMID:21654166

Dihydropteroate synthase (DHPS) gene mutation study in HIV-Infected Indian patients with Pneumocystis jirovecii pneumonia., PMID:21252456

Enhanced excitation-coupled Ca(2+) entry induces nuclear translocation of NFAT and contributes to IL-6 release from myotubes from patients with central core disease., PMID:21088110

Study of dihydropteroate synthase (DHPS) gene mutations among isolates of Pneumocystis jiroveci., PMID:19246797

[Obtaining antibodies to 1,4-dihydropyridine calcium channel blockers]., PMID:18663964

Prevalence of DHPS polymorphisms associated with sulfa resistance in South African Pneumocystis jirovecii strains., PMID:17169020

Pneumocystosis: survey and DHPS genotype analysis in 14 Parisian hospitals in 2003 and 2004., PMID:17169018

Nifedipine inhibits tumor necrosis factor-alpha-induced upregulation of monocyte chemoattractant protein-1 mRNA levels by suppressing CD40 expression in endothelial cells., PMID:15921025

Engineering dihydropteroate synthase (DHPS) for efficient expression on M13 phage., PMID:15894428

Prevention of experimental diffuse lamellar keratitis using a novel platelet-activating factor receptor antagonist., PMID:15093656

Disruption of intestinal motility by a calcium channel-stimulating autoantibody in type 1 diabetes., PMID:14988836

Development of an enzyme immunoassay for serum 16-dehydropregnenolone., PMID:11510475

Photoaffinity labeling and purification of ZG-16p, a high-affinity dihydropyridine binding protein of rat pancreatic zymogen granule membranes that regulates a K(+)-selective conductance., PMID:10648640

Effects of some 1,4-dihydropyridine Ca antagonists on the blast transformation of rat spleen lymphocytes., PMID:10377955

Effect of cerebrocrast on the lymphocyte blast transformation activity in normal and streptozotocin-induced diabetic rats., PMID:10377954

Regional expression and functional characterization of the L-type Ca2+-channel in myocardium from patients with end-stage heart failure and in non-failing human hearts., PMID:10072735

Passive transfer of Lambert-Eaton myasthenic syndrome induces dihydropyridine sensitivity of ICa in mouse motor nerve terminals., PMID:9744921

Alternative splicing of exons in the alpha1 subunit of the rat testis L-type voltage-dependent calcium channel generates germ line-specific dihydropyridine binding sites., PMID:9570267

Epitope characterization of malondialdehyde-acetaldehyde adducts using an enzyme-linked immunosorbent assay., PMID:9305579

Conditional differentiation of heart- and smooth muscle-derived cells transformed by a temperature-sensitive mutant of SV40 T antigen., PMID:8838663

Expression of calcium channel subunits in the normal and diseased human myocardium., PMID:8820405

Antisense depletion of beta-subunits modulates the biophysical and pharmacological properties of neuronal calcium channels., PMID:7537818

Temporal differences in the induction of dihydropyridine receptor subunits and ryanodine receptors during skeletal muscle development., PMID:8063821

Primary structure and expression of the dihydropteroate synthetase gene of Plasmodium falciparum., PMID:8041761

Co-localization of 1,4-dihydropyridine receptor alpha 2/delta subunit and N-CAM during early myogenesis in vitro., PMID:7929631

Immunohistochemical localization of L-type calcium channels in the developing first molar of the rat during odontoblast differentiation., PMID:8185503

Calcium channel subtypes controlling serotonin release from human small cell lung carcinoma cell lines., PMID:8253745

Phosphorylation of the L-type calcium channel beta subunit is involved in beta-adrenergic signal transduction in canine myocardium., PMID:8253200

G(o) transduces GABAB-receptor modulation of N-type calcium channels in cultured dorsal root ganglion neurons., PMID:8309795

Identification of 1,4-dihydropyridine binding domains within the primary structure of the alpha 1 subunit of the skeletal muscle L-type calcium channel., PMID:8405401

Purification of the cardiac 1,4-dihydropyridine receptor using immunoaffinity chromatography with a monoclonal antibody against the alpha 2 delta subunit of the skeletal muscle dihydropyridine receptor., PMID:1332871

1,4-Dihydropyridines modulate GTP hydrolysis by Go in neuronal membranes., PMID:1382024

Cyclic AMP-dependent phosphorylation and regulation of the cardiac dihydropyridine-sensitive Ca channel., PMID:1325377

Degradation of rat liver cytochromes P450 3A after their inactivation by 3,5-dicarbethoxy-2,6-dimethyl-4-ethyl-1,4-dihydropyridine: characterization of the proteolytic system., PMID:1497342

Structural characterization of the dihydropyridine receptor-linked calcium channel from porcine heart., PMID:1328169

Expression of dihydropyridine-sensitive brain calcium channels in the rat central nervous system., PMID:1312035

Morphological, biochemical, and electrophysiological characterization of a clonal cell (H9c2) line from rat heart., PMID:1683272

Inducible bilirubin-degrading system of rat liver microsomes: role of cytochrome P450IA1., PMID:1944239

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

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For research use only. Not for human or drug use.

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Anti-DHPS Polyclonal Antibody [PHE64801]
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