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Anti-HIV1 Gag polyprotein Nanobody (SAA0891) (RVV19303)

Host species:Alpaca
Isotype:VHH-8His-Cys-tag
Applications:ELISA
Expression system:Mammalian Cells
Overview

Catalog No.

RVV19303

Expression system

Mammalian Cells

Species reactivity

Human immunodeficiency virus 1

Host species

Alpaca

Isotype

VHH-8His-Cys-tag

Clonality

Monoclonal

Target

Gag polyprotein, Matrix protein p17, MA, gag

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Purified by Nickel column.

Accession

B6DRA0

Applications

ELISA

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

SAA0891

Data Image
  • SDS-PAGE
    SDS PAGE for HIV1 Gag polyprotein Nanobody
References

HIV Gag polyprotein: processing and early viral particle assembly. PMID: 23266279

Relationship between HIV-1 Gag Multimerization and Membrane Binding. PMID: 35337029

The Life-Cycle of the HIV-1 Gag-RNA Complex. PMID: 27626439

Budding of a Retrovirus: Some Assemblies Required. PMID: 33092109

The HIV-1 Gag Protein Displays Extensive Functional and Structural Roles in Virus Replication and Infectivity. PMID: 35886917

Recent Advances in HIV-1 Gag Inhibitor Design and Development. PMID: 32272714

How HIV-1 Gag Manipulates Its Host Cell Proteins: A Focus on Interactors of the Nucleocapsid Domain. PMID: 32823718

HIV-1 Packaging Visualised by In-Gel SHAPE. PMID: 34960658

Human immunodeficiency virus type 1 Gag polyprotein multimerization requires the nucleocapsid domain and RNA and is promoted by the capsid-dimer interface and the basic region of matrix protein. PMID: 10482606

Antiviral activity of recombinant ankyrin targeted to the capsid domain of HIV-1 Gag polyprotein. PMID: 22348230

The molecular architecture of HIV. PMID: 21762795

Dynamic interactions of the Gag polyprotein. PMID: 8791725

HIV-1 assembly, budding, and maturation. PMID: 22762019

The structural biology of HIV assembly. PMID: 18406133

Distinct Contributions of Different Domains within the HIV-1 Gag Polyprotein to Specific and Nonspecific Interactions with RNA. PMID: 32252233

Dynamics of HIV-1 Gag Processing as Revealed by Fluorescence Lifetime Imaging Microscopy and Single Virus Tracking. PMID: 35215933

Integrative structural biology of HIV-1 capsid protein assemblies: combining experiment and computation. PMID: 33901736

New insights into HIV assembly and trafficking. PMID: 21841072

Modeling the full length HIV-1 Gag polyprotein reveals the role of its p6 subunit in viral maturation and the effect of non-cleavage site mutations in protease drug resistance. PMID: 29237328

CryoET structures of immature HIV Gag reveal six-helix bundle. PMID: 33863979

Human immunodeficiency virus type 1 Gag polyprotein modulates its own translation. PMID: 17041220

Rendezvous at Plasma Membrane: Cellular Lipids and tRNA Set up Sites of HIV-1 Particle Assembly and Incorporation of Host Transmembrane Proteins. PMID: 32752131

[Membrane Binding of Retroviral Gag Proteins]. PMID: 26437838

Functional domains of HIV-1 gag-polyprotein expressed in baculovirus-infected cells. PMID: 1871977

The role of matrix in HIV-1 envelope glycoprotein incorporation. PMID: 24933691

Proteasome inhibition interferes with gag polyprotein processing, release, and maturation of HIV-1 and HIV-2. PMID: 11087859

Biochemical Reconstitution of HIV-1 Assembly and Maturation. PMID: 31801870

Overview of the Nucleic-Acid Binding Properties of the HIV-1 Nucleocapsid Protein in Its Different Maturation States. PMID: 33003650

The tRNALys packaging complex in HIV-1. PMID: 15183344

Cleavage of HIV-1 gag polyprotein synthesized in vitro: sequential cleavage by the viral protease. PMID: 2692658

[HIV-1 biogenesis unmasked]. PMID: 19038087

Binding of the human immunodeficiency virus type 1 Gag polyprotein to cyclophilin A is mediated by the central region of capsid and requires Gag dimerization. PMID: 8676452

Essential and supporting host cell factors for HIV-1 budding. PMID: 22004035

Characterization of the interaction between the HIV-1 Gag structural polyprotein and the cellular ribosomal protein L7 and its implication in viral nucleic acid remodeling. PMID: 27515235

HIV-1 assembly and maturation. PMID: 15168195

The HIV-1 assembly machine. PMID: 11816161

Conformation and dynamics of the Gag polyprotein of the human immunodeficiency virus 1 studied by NMR spectroscopy. PMID: 25713345

Role of HIV-1 Gag domains in viral assembly. PMID: 12873766

The nucleic acid chaperone activity of the HIV-1 Gag polyprotein is boosted by its cellular partner RPL7: a kinetic study. PMID: 32797159

Characterization of a myristoylated, monomeric HIV Gag protein. PMID: 19285328

HIV-1 sequences isolated from patients promote expression of shorter isoforms of the Gag polyprotein. PMID: 27659676

Mutational analysis of HIV-1 gag proteins (review). PMID: 10934287

Incorporation of Pr160(gag-pol) into virus particles requires the presence of both the major homology region and adjacent C-terminal capsid sequences within the Gag-Pol polyprotein. PMID: 9151838

NC-mediated nucleolar localization of retroviral gag proteins. PMID: 23036987

HIV-1 gag proteins: diverse functions in the virus life cycle. PMID: 9813197

Intracellular trafficking of HIV-1 Gag: how Gag interacts with cell membranes and makes viral particles. PMID: 16092502

The stoichiometry of Gag protein in HIV-1. PMID: 15208690

Perturbing HIV-1 Ribosomal Frameshifting Frequency Reveals a cis Preference for Gag-Pol Incorporation into Assembling Virions. PMID: 34643428

A Consensus View of ESCRT-Mediated Human Immunodeficiency Virus Type 1 Abscission. PMID: 28715971

Context surrounding processing sites is crucial in determining cleavage rate of a subset of processing sites in HIV-1 Gag and Gag-Pro-Pol polyprotein precursors by viral protease. PMID: 22334652

Activity of human immunodeficiency virus type 1 protease inhibitors against the initial autocleavage in Gag-Pol polyprotein processing. PMID: 22508308

Self assembly of HIV-1 Gag protein on lipid membranes generates PI(4,5)P2/Cholesterol nanoclusters. PMID: 28008947

Flexibility in the P2 domain of the HIV-1 Gag polyprotein. PMID: 15238640

p6gag domain confers cis HIV-1 Gag-Pol assembly and release capability. PMID: 26489905

Rational engineering enables co-crystallization and structural determination of the HIV-1 matrix-tRNA complex. PMID: 35005638

HIV-1 matrix mutations that alter gag membrane binding modulate mature core formation and post-entry events. PMID: 31055063

Structure of the N-terminal 283-residue fragment of the immature HIV-1 Gag polyprotein. PMID: 12032547

Specific Interaction of DARPin with HIV-1 CANTD Disturbs the Distribution of Gag, RNA Packaging, and Tetraspanin Remodelling in the Membrane. PMID: 35458554

Structural characterization of HIV-1 matrix mutants implicated in envelope incorporation. PMID: 33485964

Elements in HIV-1 Gag contributing to virus particle assembly. PMID: 23099087

Kinesin KIF4 regulates intracellular trafficking and stability of the human immunodeficiency virus type 1 Gag polyprotein. PMID: 18684836

Structure and assembly of immature HIV. PMID: 19549863

Proteomic analysis of HIV-1 Gag interacting partners using proximity-dependent biotinylation. PMID: 26362536

RNA aptamers directed to human immunodeficiency virus type 1 Gag polyprotein bind to the matrix and nucleocapsid domains and inhibit virus production. PMID: 20980522

Double trouble? Gag in conjunction with double insert in HIV protease contributes to reduced DRV susceptibility. PMID: 30573649

Specific inter-domain interactions stabilize a compact HIV-1 Gag conformation. PMID: 31437199

Ubiquitination of HIV-1 and MuLV Gag. PMID: 11112487

Assembly of the HIV-1 core particle. PMID: 12152518

Molecular mechanisms by which HERV-K Gag interferes with HIV-1 Gag assembly and particle infectivity. PMID: 28446240

The role of cellular factors in promoting HIV budding. PMID: 21762798

Virus Hijacks Host Proteins and Machinery for Assembly and Budding, with HIV-1 as an Example. PMID: 35891508

Dynamics of HIV-1 assembly and release. PMID: 19893629

Gag-Pol Transframe Domain p6* Is Essential for HIV-1 Protease-Mediated Virus Maturation. PMID: 26030443

Datasheet
$ 372
Product specifications
100 μg 372 1 mg 1860

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Anti-HIV1 Gag polyprotein Nanobody (SAA0891) [RVV19303]
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