Catalog No.
PXX09901
Species reactivity
Escherichia coli O127:H6 (strain E2348/69 / EPEC)
Host species
Rabbit
Isotype
IgG
Clonality
Polyclonal
Immunogen
E. coli - derived recombinant Escherichia coli eae/Intimin (Lys450-Gln655).
Tested applications
ELISA: 1:4000-1:8000, IHC: 1:50-1:200, WB: 1:1000-1:4000
Target
Intimin, Attaching and effacing protein, Eae protein, eae, eaeA, E2348C_3939
Concentration
1.7 mg/ml
Purification
Purified by antigen affinity column.
Accession
P19809
Applications
ELISA, IHC, WB
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Isolation of Shiga toxin-producing Escherichia coli O157 and non-O157 from retail imported frozen beef marketed in Saudi Arabia using immunomagnetic separation and multiplex PCR., PMID:40271089
High-yield production of recombinant human myelin oligodendrocyte glycoprotein in SHuffle bacteria without a refolding step., PMID:39406335
Detection of Shiga toxin-producing Escherichia coli in dairy cows: genetic characterization and inhibition of adherence by cattle anti-STEC antibodies to HEp-2 cell., PMID:39331343
Shiga Toxin (Stx) Type 1a and Stx2a Translocate through a Three-Layer Intestinal Model., PMID:36977098
Effect of the administration of Lactobacillus spp. strains on neonatal diarrhoea, immune parameters and pathogen abundance in pre-weaned calves., PMID:32877228
Heterologous expression of Intimin and IpaB fusion protein in Lactococcus lactis and its mucosal delivery elicit protection against pathogenicity of Escherichia coli O157 and Shigella flexneri in a murine model., PMID:32464569
Epitope mapping of anti-myelin oligodendrocyte glycoprotein (MOG) antibodies in a mouse model of multiple sclerosis: microwave-assisted synthesis of the peptide antigens and ELISA screening., PMID:26663200
Encoded protein from ycbR gene of enterohemorrhagic Escherichia coli O157:H7 associated with adherence to HEp-2 cells., PMID:24680289
Adhesion of enterohemorrhagic Escherichia coli O157:H7 to the intestinal epithelia is essential for inducing secretory IgA antibody production in the intestine of mice., PMID:24583860
Action of phosphorylated mannanoligosaccharides on immune and hematological responses and fecal consistency of dogs experimentally infected with enteropathogenic Escherichia coli strains., PMID:24294246
Sensitive and specific detection of enteropathogenic and enterohemorrhagic Escherichia coli using recombinant anti-intimin antibody by immunofluorescence assay., PMID:24095642
Determination of enterohemorrhagic Escherichia coli serotype O165:HNM infection in a hemolytic uremic syndrome patient with adenovirus seroype 41., PMID:24047737
Dissection of the role of pili and type 2 and 3 secretion systems in adherence and biofilm formation of an atypical enteropathogenic Escherichia coli strain., PMID:23897608
A directed intimin insertion mutant of a rabbit enteropathogenic Escherichia coli (REPEC) is attenuated, immunogenic and elicits serogroup specific protection., PMID:23084628
A DNA vaccine against Escherichia coli O157:H7., PMID:23023214
Oral immunization with attenuated Salmonella vaccine expressing Escherichia coli O157:H7 intimin gamma triggers both systemic and mucosal humoral immunity in mice., PMID:22671942
Epidemiology of Shiga toxin-producing Escherichia coli O157 in very young calves in the North Island of New Zealand., PMID:22175425
Carbon nanoparticles as detection labels in antibody microarrays. Detection of genes encoding virulence factors in Shiga toxin-producing Escherichia coli., PMID:21936575
Carbon nanoparticles in lateral flow methods to detect genes encoding virulence factors of Shiga toxin-producing Escherichia coli., PMID:21046083
Immunogenic properties of chimeric protein from espA, eae and tir genes of Escherichia coli O157:H7., PMID:20709010
Distribution and phylogeny of immunoglobulin-binding protein G in Shiga toxin-producing Escherichia coli and its association with adherence phenotypes., PMID:20547747
Development of a live oral attaching and effacing Escherichia coli vaccine candidate using Vibrio cholerae CVD 103-HgR as antigen vector., PMID:19878715
Identification of enteropathogenic and enterohaemorrhagic Escherichia coli strains by immunoserological detection of intimin., PMID:19709337
Virulence features of atypical enteropathogenic Escherichia coli identified by the eae(+) EAF-negative stx(-) genetic profile., PMID:19442475
Attaching and effacing Escherichia coli downregulate DNA mismatch repair protein in vitro and are associated with colorectal adenocarcinomas in humans., PMID:19436735
Protective effect of a synapsin peptide genetically fused to the B subunit of Escherichia coli heat-labile enterotoxin in rat autoimmune encephalomyelitis., PMID:19267420
Production and characterization of rabbit polyclonal sera against Shiga toxins Stx1 and Stx2 for detection of Shiga toxin-producing Escherichia coli., PMID:18822082
[The epidemiology of hemolytic uremic syndrome in Argentina. Diagnosis of the etiologic agent, reservoirs and routes of transmission]., PMID:17354474
Development of a simple latex agglutination assay for detection of shiga toxin-producing Escherichia coli (STEC) by using polyclonal antibody against STEC., PMID:17344348
O148 Shiga toxin-producing Escherichia coli outbreak: microbiological investigation as a useful complement to epidemiological investigation., PMID:16961636
Protection against hemorrhagic colitis in an animal model by oral immunization with isogeneic rabbit enteropathogenic Escherichia coli attenuated by truncating intimin., PMID:16177337
Isolation of recombinant antibodies against EspA and intimin of Escherichia coli O157:H7., PMID:15243046
Characterization of the novel factor paa involved in the early steps of the adhesion mechanism of attaching and effacing Escherichia coli., PMID:12874331
Detection of Escherichia coli O157:H7 in raw meat by immunomagnetic separation and multiplex PCR., PMID:12708821
Flagellin of enteropathogenic Escherichia coli stimulates interleukin-8 production in T84 cells., PMID:12654834
Intimin type influences the site of human intestinal mucosal colonisation by enterohaemorrhagic Escherichia coli O157:H7., PMID:11788556
[Hemolytic-uremic syndrome: microbiological aspects]., PMID:11582928
Occurrence and characterization of Escherichia coli O157 isolated from cattle in Norway., PMID:11214668
Nontoxigenic sorbitol-fermenting Escherichia coli O157:H- associated with a family outbreak of diarrhoea., PMID:11098536
Haemolytic uraemic syndrome and Shiga toxin-producing Escherichia coli infection in children in France. The Société de Néphrologie Pédiatrique., PMID:10813145
Human colostrum and serum contain antibodies reactive to the intimin-binding region of the enteropathogenic Escherichia coli translocated intimin receptor., PMID:10630443
[Escherichia coli O157:H7, an emerging pathogen]., PMID:10605481
Hierarchy in the expression of the locus of enterocyte effacement genes of enteropathogenic Escherichia coli., PMID:10594820
Development of a universal intimin antiserum and PCR primers., PMID:10565891
Escherichia coli O157:H7 and O157:H(-) strains that do not produce Shiga toxin: phenotypic and genetic characterization of isolates associated with diarrhea and hemolytic-uremic syndrome., PMID:10523540
Molecular characterization of a Shiga toxigenic Escherichia coli O113:H21 strain lacking eae responsible for a cluster of cases of hemolytic-uremic syndrome., PMID:10488206
Use of a monoclonal antibody against an Escherichia coli O26 surface protein for detection of enteropathogenic and enterohemorrhagic strains., PMID:10391872
Detection of intimins alpha, beta, gamma, and delta, four intimin derivatives expressed by attaching and effacing microbial pathogens., PMID:9508292
Effect of prior experimental human enteropathogenic Escherichia coli infection on illness following homologous and heterologous rechallenge., PMID:9423838
Gastric attaching and effacing Escherichia coli lesions in a puppy with naturally occurring enteric colibacillosis and concurrent canine distemper virus infection., PMID:8952035
For research use only. Not for human or drug use.
