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Anti-Human NMT1 Polyclonal Antibody

Catalog #:   PHD89001 Specific References (17) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: P30419
Overview

Catalog No.

PHD89001

Species reactivity

Human

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human NMT1 (Thr108-Gln496).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

Myristoyl-CoA:protein N-myristoyltransferase 1, Type I N-myristoyltransferase, NMT 1, Peptide N-myristoyltransferase 1, NMT1, Glycylpeptide N-tetradecanoyltransferase 1, NMT

Purification

Purified by antigen affinity column.

Accession

P30419

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • Western Blot
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with NMT1 antibody (PHD89001) at 1 μg/ml.

    Lane 1: Recombinant protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 48 kDa
    Observed MW: 48 kDa
References

Myristoylation of TMEM106B by NMT1/2 regulates TMEM106B trafficking and turnover., PMID:40451428

N-myristoyltransferase proteins in breast cancer: prognostic relevance and validation as a new drug target., PMID:33398478

N-myristoyltransferase-1 is necessary for lysosomal degradation and mTORC1 activation in cancer cells., PMID:32686708

Palladium based nanoparticles for the treatment of advanced melanoma., PMID:30824801

Myristoylation confers noncanonical AMPK functions in autophagy selectivity and mitochondrial surveillance., PMID:26272043

A new, robust, and nonradioactive approach for exploring N-myristoylation., PMID:22829651

Intracellular expression of recombinant antibody fluorescent protein fusions for localization of target antigens in Schizosaccharomyces pombe., PMID:16118428

Characterization and expression of the Neurospora crassa nmt-1 gene., PMID:13680155

High level activation of vitamin B1 biosynthesis genes in haustoria of the rust fungus Uromyces fabae., PMID:10830262

Vectors for the expression of tagged proteins in Schizosaccharomyces pombe., PMID:9852950

N-myristoylation of Arf proteins in Candida albicans: an in vivo assay for evaluating antifungal inhibitors of myristoyl-CoA: protein N-myristoyltransferase., PMID:9043113

Binding of site-directed monoclonal antibodies to an epitope located in the A/B region (amino acids 140-154) of human estrogen receptor-induced conformational changes in an epitope in the DNA-binding domain., PMID:8883222

Naturally-occurring anti-thymocyte autoantibody which identifies a restricted CD4+CD8+CD3-/lo/int thymocyte subpopulation exhibits extensive polyspecificity., PMID:8728353

Monoclonal antibody to a Thy-1 related/associated molecule identifies a subpopulation of immature mouse thymocytes., PMID:7940641

Establishment and characterization of a rat yolk sac tumor cell line, NMT-1, producing alpha-fetoprotein, with potential for lymphatic metastasis., PMID:7507475

Characterization of the specificity of a naturally-occurring monoclonal anti-thymocyte autoantibody derived from an unimmunized, neonatal Balb/c mouse., PMID:8284806

Analysis of the compartmentalization of myristoyl-CoA:protein N-myristoyltransferase in Saccharomyces cerevisiae., PMID:1544917

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

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Distributor list

For research use only. Not for human or drug use.

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Anti-Human NMT1 Polyclonal Antibody [PHD89001]
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