Anti-Human PIP5K1A Polyclonal Antibody

Catalog No.

PHK68901

Species reactivity

Human

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human PIP5K1A (Lys62-Lys452).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

PIP5K1-alpha, PtdIns(4)P-5-kinase 1 alpha, Phosphatidylinositol 4-phosphate 5-kinase type-1 alpha, PIP5KIalpha, 68 kDa type I phosphatidylinositol 4-phosphate 5-kinase alpha, PIP5K1A, Phosphatidylinositol 4-phosphate 5-kinase type I alpha

Purification

Purified by antigen affinity column.

Accession

Q99755

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

β2-Microglobulin Maintains Glioblastoma Stem Cells and Induces M2-like Polarization of Tumor-Associated Macrophages., PMID:35841593

FcγRIIIa receptor interacts with androgen receptor and PIP5K1α to promote growth and metastasis of prostate cancer., PMID:34932854

Distribution and localization of phosphatidylinositol 5-phosphate, 4-kinase alpha and beta in the brain., PMID:32449185

SUMOylation is required for PIPK1γ-driven keratinocyte migration and growth., PMID:31276292

Multistep Compositional Remodeling of Supported Lipid Membranes by Interfacially Active Phosphatidylinositol Kinases., PMID:27118725

FAK, talin and PIPKIγ regulate endocytosed integrin activation to polarize focal adhesion assembly., PMID:27043085

LPS-induced clustering of CD14 triggers generation of PI(4,5)P2., PMID:26446256

Inositol phosphate pathway controls transcription of telomeric expression sites in trypanosomes., PMID:25964327

TGF-β directs trafficking of the epithelial sodium channel ENaC which has implications for ion and fluid transport in acute lung injury., PMID:24324142

Involvement of PtdIns(4,5)P2 in the regulatory mechanism of small intestinal P-glycoprotein expression., PMID:24311454

Collaboration of AMPK and PKC to induce phosphorylation of Ser413 on PIP5K1B resulting in decreased kinase activity and reduced PtdIns(4,5)P2 synthesis in response to oxidative stress and energy restriction., PMID:23909401

Uptake of advanced glycation end products by proximal tubule epithelial cells via macropinocytosis., PMID:23747564

Phosphatidylinositol 4-phosphate 5-kinase β regulates growth cone morphology and semaphorin 3A-triggered growth cone collapse in mouse dorsal root ganglion neurons., PMID:23680464

Endosomal type Iγ PIP 5-kinase controls EGF receptor lysosomal sorting., PMID:23602387

Integrin α9β1 in airway smooth muscle suppresses exaggerated airway narrowing., PMID:22772469

Multiple post-translational modifications regulate E-cadherin transport during apoptosis., PMID:22375065

Phosphatidylinositol 4-phosphate 5-kinase alpha (PIPKα) regulates neuronal microtubule depolymerase kinesin, KIF2A and suppresses elongation of axon branches., PMID:22307638

PIPKI gamma 90 negatively regulates LFA-1-mediated adhesion and activation in antigen-induced CD4+ T cells., PMID:20855869

Enhanced phosphatidylinositol 4-phosphate 5-kinase alpha expression and PI(4,5)P2 production in LPS-stimulated microglia., PMID:20659513

Phosphatidylinositol 4,5-bisphosphate and PIP5-kinase Ialpha are required for invadopodia formation in human breast cancer cells., PMID:20426790

FAK, PIP5KIgamma and gelsolin cooperatively mediate force-induced expression of alpha-smooth muscle actin., PMID:19596799

Two novel phosphatidylinositol-4-phosphate 5-kinase type Igamma splice variants expressed in human cells display distinctive cellular targeting., PMID:19548880

Assaying endogenous phosphatidylinositol-4-phosphate 5-kinase (PIP5K) activities., PMID:19160683

Tracking the transport of E-cadherin to and from the plasma membrane., PMID:19066034

PI4P5-kinase Ialpha is required for efficient HIV-1 entry and infection of T cells., PMID:18981107

Enzymes phosphorylating lipids and polysaccharides., PMID:17511601

Inositol 1,4,5 trisphosphate receptor and chromogranin B are concentrated in different regions of the hippocampus., PMID:17471556

Phospholipase D2 stimulates integrin-mediated adhesion via phosphatidylinositol 4-phosphate 5-kinase Igamma b., PMID:15976455

Arf6: a new player in FcgammaRIIIA lymphocyte-mediated cytotoxicity., PMID:15817676

The direct interaction between ASH2, a Drosophila trithorax group protein, and SKTL, a nuclear phosphatidylinositol 4-phosphate 5-kinase, implies a role for phosphatidylinositol 4,5-bisphosphate in maintaining transcriptionally active chromatin., PMID:15280236

BTK regulates PtdIns-4,5-P2 synthesis: importance for calcium signaling and PI3K activity., PMID:14614854

Protein kinase C mediates translocation of type II phosphatidylinositol 5-phosphate 4-kinase required for platelet alpha-granule secretion., PMID:12509423

Inhibition of phosphatidylinositol-4-phosphate 5-kinase Ialpha impairs localized actin remodeling and suppresses phagocytosis., PMID:12223494

PIP kinase Igamma is the major PI(4,5)P(2) synthesizing enzyme at the synapse., PMID:11604140

Phosphatidylinositol 4,5-bisphosphate mediates Ca2+-induced platelet alpha-granule secretion: evidence for type II phosphatidylinositol 5-phosphate 4-kinase function., PMID:11304526

Localized biphasic changes in phosphatidylinositol-4,5-bisphosphate at sites of phagocytosis., PMID:11134066

Coupled inositide phosphorylation and phospholipase D activation initiates clathrin-coat assembly on lysosomes., PMID:10364223

Phosphoinositide signaling pathways in nuclei are associated with nuclear speckles containing pre-mRNA processing factors., PMID:9843587

Type I phosphatidylinositol-4-phosphate 5-kinases are distinct members of this novel lipid kinase family., PMID:8955136

Physical association of the small GTPase Rho with a 68-kDa phosphatidylinositol 4-phosphate 5-kinase in Swiss 3T3 cells., PMID:8868471

FKBP12-rapamycin target TOR2 is a vacuolar protein with an associated phosphatidylinositol-4 kinase activity., PMID:8846782

The cloning and sequence of the C isoform of PtdIns4P 5-kinase., PMID:7639683

ATP-dependent inositide phosphorylation required for Ca(2+)-activated secretion., PMID:7877690

Type I phosphatidylinositol 4-phosphate 5-kinase isoforms are specifically stimulated by phosphatidic acid., PMID:8157686

Monoclonal antibodies to phosphatidylinositol 4-phosphate 5-kinase: distribution and intracellular localization of the C isoform., PMID:8385940

Purification and characterization of phosphatidylinositol 4-phosphate 5-kinases., PMID:1334412

A differential location of phosphoinositide kinases, diacylglycerol kinase, and phospholipase C in the nuclear matrix., PMID:1312084

Assessment of the in situ tyrosine kinase activity of mutant insulin receptors lacking tyrosine autophosphorylation sites 1162 and 1163., PMID:1710030

Interaction between the epidermal growth factor receptor and phosphoinositide kinases., PMID:1845983

The human erythrocyte contains two forms of phosphatidylinositol-4-phosphate 5-kinase which are differentially active toward membranes., PMID:2170402

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