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Anti-Human SEC13 Polyclonal Antibody

Catalog #:   PHF13101 Specific References (17) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: P55735
Overview

Catalog No.

PHF13101

Species reactivity

Human

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human SEC13 (Met15-Gln322).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

D3S1231E, SEC13L1, SEC13, SEC13-like protein 1, SEC13-related protein, Protein SEC13 homolog, SEC13R, GATOR complex protein SEC13, SEC13A

Purification

Purified by antigen affinity column.

Accession

P55735

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • Western Blot
    Various lysates were subjected to SDS PAGE followed by western blot with SEC13 antibody (PHF13101) at 1μg/ml.

    Lane 1: RAW264.7 cell lysate
    Lane 2: HepG2 cell lysate
    Lane 3: Hela cell lysate
    Lane 4: U87 cell lysate
    Lane 5: 293T cell lysate
    Lane 6: MCF-7 cell lysate
    Lane 7: Mouse kidney lysate
    Lane 8: Mouse liver lysate

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 36 kDa
    Observed MW: 36 kDa
References

High levels of intracellular endotrophin in adipocytes mediate COPII vesicle supplies to autophagosome to impair autophagic flux and contribute to systemic insulin resistance in obesity., PMID:37302692

Sar1 Interacts with Sec23/Sec24 and Sec13/Sec31 Complexes: Insight into Its Involvement in the Assembly of Coat Protein Complex II in the Microsporidian Nosema bombycis., PMID:36301095

SEC23B is required for the maintenance of murine professional secretory tissues., PMID:22745161

Phosphorylation of Sar1b protein releases liver fatty acid-binding protein from multiprotein complex in intestinal cytosol enabling it to bind to endoplasmic reticulum (ER) and bud the pre-chylomicron transport vesicle., PMID:22303004

[Analysis of expression of vesicular transport genes in avesicular cells of the microsporidium Paranosema (Antonospora) locustae]., PMID:20302012

Dynamics of COPII vesicles and the Golgi apparatus in cultured Nicotiana tabacum BY-2 cells provides evidence for transient association of Golgi stacks with endoplasmic reticulum exit sites., PMID:15805489

Mammalian Bet3 functions as a cytosolic factor participating in transport from the ER to the Golgi apparatus., PMID:15728249

The entire Nup107-160 complex, including three new members, is targeted as one entity to kinetochores in mitosis., PMID:15146057

COPII proteins are required for Golgi fusion but not for endoplasmic reticulum budding of the pre-chylomicron transport vesicle., PMID:12482926

Selective protein exit from yeast endoplasmic reticulum in absence of functional COPII coat component Sec13p., PMID:12475940

Novel vertebrate nucleoporins Nup133 and Nup160 play a role in mRNA export., PMID:11684705

Cellular COPII proteins are involved in production of the vesicles that form the poliovirus replication complex., PMID:11559814

Mammalian homologues of yeast sec31p. An ubiquitously expressed form is localized to endoplasmic reticulum (ER) exit sites and is essential for ER-Golgi transport., PMID:10788476

A family of mammalian proteins homologous to yeast Sec24p., PMID:10329445

The mammalian homolog of yeast Sec13p is enriched in the intermediate compartment and is essential for protein transport from the endoplasmic reticulum to the Golgi apparatus., PMID:8972206

Human SEC13Rp functions in yeast and is located on transport vesicles budding from the endoplasmic reticulum., PMID:7876304

The Sec13p complex and reconstitution of vesicle budding from the ER with purified cytosolic proteins., PMID:8223424

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

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For research use only. Not for human or drug use.

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Anti-Human SEC13 Polyclonal Antibody [PHF13101]
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