Catalog No.
PHK63201
Species reactivity
Human
Host species
Rabbit
Isotype
IgG
Clonality
Polyclonal
Immunogen
E. coli - derived recombinant Human MAD1L1 (His544-Ala718).
Tested applications
ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000
Target
HsMAD1, MAD1-like protein 1, hMAD1, Mitotic arrest deficient 1-like protein 1, Tax-binding protein 181, MAD1, TXBP181, Mitotic checkpoint MAD1 protein homolog, MAD1L1, Mitotic spindle assembly checkpoint protein MAD1
Purification
Purified by antigen affinity column.
Accession
Q9Y6D9
Applications
ELISA, IHC, WB
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Association Between Peptide Antigen-Related Antibody Levels and the Short- and Long-Term Efficacy of Antipsychotic Treatment in Drug-Naïve First-Episode Schizophrenia Patients., PMID:39673496
The closed form of Mad2 is bound to Mad1 and Cdc20 at unattached kinetochores., PMID:29895228
Study of Novel Autoantibodies in Schizophrenia., PMID:29373732
Distinct domains in Bub1 localize RZZ and BubR1 to kinetochores to regulate the checkpoint., PMID:26031201
Structure of human Mad1 C-terminal domain reveals its involvement in kinetochore targeting., PMID:22493223
Probing the in vivo function of Mad1:C-Mad2 in the spindle assembly checkpoint., PMID:21772247
PRP4 is a spindle assembly checkpoint protein required for MPS1, MAD1, and MAD2 localization to the kinetochores., PMID:17998396
Localization of mitotic arrest deficient 1 (MAD1) in mouse oocytes during the first meiosis and its functions as a spindle checkpoint protein., PMID:15342357
Dynamics of centromere and kinetochore proteins; implications for checkpoint signaling and silencing., PMID:15182667
Inhibition of the epidermal growth factor receptor suppresses telomerase activity in HSC-1 human cutaneous squamous cell carcinoma cells., PMID:14708611
Spindle checkpoint proteins Mad1 and Mad2 are required for cytostatic factor-mediated metaphase arrest., PMID:14691134
Nuf2 and Hec1 are required for retention of the checkpoint proteins Mad1 and Mad2 to kinetochores., PMID:14654001
Anaphase onset does not require the microtubule-dependent depletion of kinetochore and centromere-binding proteins., PMID:12235289
Mad2 binding to Mad1 and Cdc20, rather than oligomerization, is required for the spindle checkpoint., PMID:11707408
Expression of Mad1 in T cells leads to reduced thymic cellularity and impaired mitogen-induced proliferation., PMID:11313860
Contrasting localization of c-Myc with other Myc superfamily transcription factors in the human hair follicle and during the hair growth cycle., PMID:11286633
Mitotic checkpoint proteins HsMAD1 and HsMAD2 are associated with nuclear pore complexes in interphase., PMID:11181178
Co-induction of Mad1 and c-Myc in activated normal B lymphocytes., PMID:10849366
Analysis of the DNA-binding activities of Myc/Max/Mad network complexes during induced differentiation of U-937 monoblasts and F9 teratocarcinoma cells., PMID:9264414
Inhibition of c-myc expression induces apoptosis of WEHI 231 murine B cells., PMID:8756660
Mad1p, a phosphoprotein component of the spindle assembly checkpoint in budding yeast., PMID:7593191
For research use only. Not for human or drug use.
