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Anti-Human RPA2 Polyclonal Antibody

Catalog #:   PHD14101 Specific References (22) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: P15927
Overview

Catalog No.

PHD14101

Species reactivity

Human

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human RPA2 (Thr50-Glu270).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

RPA34, Replication protein A 34 kDa subunit, Replication protein A 32 kDa subunit, RF-A protein 2, RP-A p34, Replication factor A protein 2, REPA2, RPA2, RPA32, RP-A p32

Concentration

1.12 mg/ml

Purification

Purified by antigen affinity column.

Accession

P15927

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • Western Blot
    Various lysates were subjected to SDS PAGE followed by western blot with RPA2 antibody (PHD14101) at 1μg/ml.

    Lane 1: NIH3T3 cell lysate
    Lane 2: Hela cell lysate
    Lane 3: Jurkat cell lysate
    Lane 4: Molt-4 cell lysate
    Lane 5: THP-1 cell lysate
    Lane 6: Lncap cell lysate
    Lane 7: 293T cell lysate

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 30 kDa
    Observed MW: 30 kDa
References

Replication Protein A (RPA1, RPA2 and RPA3) expression in gastric cancer: correlation with clinicopathologic parameters and patients' survival., PMID:32862594

Pea (Pisum sativum) allergy in children: Pis s 1 is an immunodominant major pea allergen and presents IgE binding sites with potential diagnostic value., PMID:32078204

PBMCs transcriptome profiles identified breed-specific transcriptome signatures for PRRSV vaccination in German Landrace and Pietrain pigs., PMID:31536525

Author Correction: Per2 induction limits lymphoid-biased haematopoietic stem cells and lymphopoiesis in the context of DNA damage and ageing., PMID:30808996

Identification of stroke-associated-antigens via screening of recombinant proteins from the human expression cDNA library (SEREX)., PMID:25890248

Leucine methylation of protein phosphatase PP4C at C-terminal is critical for its cellular functions., PMID:25130464

Interplay of DNA damage and cell cycle signaling at the level of human replication protein A., PMID:25091156

Activation of WIP1 phosphatase by HTLV-1 Tax mitigates the cellular response to DNA damage., PMID:23405243

Protein interaction and cellular localization of human CDC45., PMID:23364835

Parvovirus B19 infection of human primary erythroid progenitor cells triggers ATR-Chk1 signaling, which promotes B19 virus replication., PMID:21680529

Analysis of protein phosphorylation in cisplatin-treated human cells following annexin V-based separation and multi-antibody screening., PMID:20952762

A PP4 phosphatase complex dephosphorylates RPA2 to facilitate DNA repair via homologous recombination., PMID:20154705

Ionizing radiation-dependent and independent phosphorylation of the 32-kDa subunit of replication protein A during mitosis., PMID:19671522

Immunolocalization of Kim-1, RPA-1, and RPA-2 in kidney of gentamicin-, mercury-, or chromium-treated rats: relationship to renal distributions of iNOS and nitrotyrosine., PMID:18441258

NBS1 mediates ATR-dependent RPA hyperphosphorylation following replication-fork stall and collapse., PMID:18003706

Sequential and synergistic modification of human RPA stimulates chromosomal DNA repair., PMID:17928296

Functional characterization of replication protein A2 (RPA2) from Cryptosporidium parvum., PMID:15133081

Replication protein A (RPA) phosphorylation prevents RPA association with replication centers., PMID:14966274

The second largest subunit of Trypanosoma brucei's multifunctional RNA polymerase I has a unique N-terminal extension domain., PMID:12615318

The 32-kilodalton subunit of replication protein A interacts with menin, the product of the MEN1 tumor suppressor gene., PMID:12509449

Heterogeneous expression and functional analysis of two distinct replication protein A large subunits from Cryptosporidium parvum., PMID:12392913

RPA-2.10: an anti-CD2 monoclonal antibody that inhibits alloimmune responses and monitors T cell activation., PMID:2908548

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

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For research use only. Not for human or drug use.

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Anti-Human RPA2 Polyclonal Antibody [PHD14101]
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