Catalog No.
PHA97101
Species reactivity
Human
Host species
Rabbit
Isotype
IgG
Clonality
Polyclonal
Immunogen
E. coli - derived recombinant Human RAD1 (Met1-Ser282).
Tested applications
ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000
Target
DNA repair exonuclease rad1 homolog, Rad1-like DNA damage checkpoint protein, RAD1, REC1, hRAD1, Cell cycle checkpoint protein RAD1
Purification
Purified by antigen affinity column.
Accession
O60671
Applications
ELISA, IHC, WB
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Vitamin D Receptor Promotes Global Nucleotide Excision Repair by Facilitating XPC Dissociation from Damaged DNA., PMID:33524369
Identification and characterization of a novel nuclear structure containing members of the homologous recombination and DNA damage response pathways., PMID:30553479
A novel antibody-based approach to detect the functional ERCC1-202 isoform., PMID:29482102
Randomized Prospective Biomarker Trial of ERCC1 for Comparing Platinum and Nonplatinum Therapy in Advanced Non-Small-Cell Lung Cancer: ERCC1 Trial (ET)., PMID:27893326
RPA-Binding Protein ETAA1 Is an ATR Activator Involved in DNA Replication Stress Response., PMID:27818175
ERCC1 is a prognostic biomarker in locally advanced head and neck cancer: results from a randomised, phase II trial., PMID:24064970
A role for Myh1 in DNA repair after treatment with strand-breaking and crosslinking chemotherapeutic agents., PMID:23677513
Assessment of ERCC1 and XPF protein expression using quantitative immunohistochemistry in nasopharyngeal carcinoma patients undergoing curative intent treatment., PMID:23182703
The rad1 gene in Rainbow Trout (Oncorhynchus mykiss) is highly conserved and may express proteins from non-canonical spliced isoforms., PMID:22985532
Gene expression changes in human islets exposed to type 1 diabetic serum., PMID:22885994
The RAD9-RAD1-HUS1 (9.1.1) complex interacts with WRN and is crucial to regulate its response to replication fork stalling., PMID:22002307
DNA repair biomarkers predict response to neoadjuvant chemoradiotherapy in esophageal cancer., PMID:22000749
Killing of chronic lymphocytic leukemia by the combination of fludarabine and oxaliplatin is dependent on the activity of XPF endonuclease., PMID:21632856
Rad is a p53 direct transcriptional target that inhibits cell migration and is frequently silenced in lung carcinoma cells., PMID:21221513
Mislocalization of XPF-ERCC1 nuclease contributes to reduced DNA repair in XP-F patients., PMID:20221251
1-Methyl-4-phenyl-pyridinium time-dependently alters expressions of oxoguanine glycosylase 1 and xeroderma pigmentosum group F protein in PC12 cells., PMID:20101267
Lung inflammation is associated with reduced pulmonary nucleotide excision repair in vivo., PMID:19917589
Immunodetection of DNA repair endonuclease ERCC1-XPF in human tissue., PMID:19723666
ATR and Rad17 collaborate in modulating Rad9 localisation at sites of DNA damage., PMID:19020305
Deficiency in incisions produced by XPF at the site of a DNA interstrand cross-link in Fanconi anemia cells., PMID:18020456
Nonerythroid alphaII spectrin is required for recruitment of FANCA and XPF to nuclear foci induced by DNA interstrand cross-links., PMID:12571280
Regulation of ATR substrate selection by Rad17-dependent loading of Rad9 complexes onto chromatin., PMID:11799063
Contribution of XPF functional domains to the 5' and 3' incisions produced at the site of a psoralen interstrand cross-link., PMID:11790111
L-plastin peptide activation of alpha(v)beta(3)-mediated adhesion requires integrin conformational change and actin filament disassembly., PMID:11278342
Quantitative determination of the expression of xeroderma pigmentosum F gene in human nonmelanoma skin cancers., PMID:10873627
Expression of the Fpg protein of Escherichia coli in Saccharomyces cerevisiae: effects on spontaneous mutagenesis and sensitivity to oxidative DNA damage., PMID:10717388
Interaction of the Ras-related protein associated with diabetes rad and the putative tumor metastasis suppressor NM23 provides a novel mechanism of GTPase regulation., PMID:10611312
Identification of Rad's effector-binding domain, intracellular localization, and analysis of expression in Pima Indians., PMID:9178102
Muscle Rad expression and human metabolism: potential role of the novel Ras-related GTPase in energy expenditure and body composition., PMID:9032101
Identification of functional domains within the RAD1.RAD10 repair and recombination endonuclease of Saccharomyces cerevisiae., PMID:8702799
The yeast NOP4 gene product is an essential nucleolar protein required for pre-rRNA processing and accumulation of 60S ribosomal subunits., PMID:8039505
Evidence for a repair enzyme complex involving ERCC1 and complementing activities of ERCC4, ERCC11 and xeroderma pigmentosum group F., PMID:8253091
Co-correction of the ERCC1, ERCC4 and xeroderma pigmentosum group F DNA repair defects in vitro., PMID:8253090
Specific complex formation between proteins encoded by the yeast DNA repair and recombination genes RAD1 and RAD10., PMID:1518857
Cloning and analysis of a gene involved in DNA repair and recombination, the rad1 gene of Schizosaccharomyces pombe., PMID:2355921
Excision repair of UV radiation-induced DNA damage in Caenorhabditis elegans., PMID:2767423
Overexpression of the RAD2 gene of S. cerevisiae: identification and preliminary characterization of Rad2 protein., PMID:3332971
For research use only. Not for human or drug use.
