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Anti-Human GNAT1 Polyclonal Antibody

Catalog #:   PHC89101 Specific References (13) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: P11488
Overview

Catalog No.

PHC89101

Species reactivity

Human

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human GNAT1 (Met1-Phe350).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

Transducin alpha-1 chain, GNATR, Guanine nucleotide-binding protein G(t) subunit alpha-1, GNAT1

Purification

Purified by antigen affinity column.

Accession

P11488

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • WESTERN-BLOT
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with GNAT1 antibody (PHC89101) at 1 μg/ml.

    Lane 1: Recombinant protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 42 kDa
    Observed MW: 42 kDa
  • Western Blot
    Various lysates were subjected to SDS PAGE followed by western blot with GNAT1 antibody (PHC89101) at 1 μg/ml.

    Lane 1: Human retina

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 40 kDa
    Observed MW: 40 kDa
  • Immunohistochemical
    Immunohistochemical analysis of mouse heart stained for GNAT1 with PHC89101.
References

ICAM-1 on the luminal surface of endothelial cells is induced to a greater extent in mouse retina than in other tissues in diabetes., PMID:35852587

A Naturally Occurring Canine Model of Autosomal Recessive Congenital Stationary Night Blindness., PMID:26368928

Phosphorylation of phosducin accelerates rod recovery from transducin translocation., PMID:22491418

Moderate light-induced degeneration of rod photoreceptors with delayed transducin translocation in shaker1 mice., PMID:21447681

Light-dependent phosphorylation of the gamma subunit of cGMP-phophodiesterase (PDE6gamma) at residue threonine 22 in intact photoreceptor neurons., PMID:19878658

A nonsense mutation in Gnat1, encoding the alpha subunit of rod transducin, in spontaneous mouse models of retinal dysfunction., PMID:19766629

Differentially expressed genes identified by cross-species microarray in the blind cavefish Astyanax., PMID:21392280

Light threshold-controlled cone alpha-transducin translocation., PMID:17591908

A critical role of CaBP4 in the cone synapse., PMID:16249514

Light-dependent redistribution of visual arrestins and transducin subunits in mice with defective phototransduction., PMID:12802257

Preparation and characterization of monoclonal antibodies to several frog rod outer segment proteins., PMID:6387041

A monoclonal antibody to guanine nucleotide binding protein inhibits the light-activated cyclic GMP pathway in frog rod outer segments., PMID:6092516

Immunologic and biochemical properties of several retinal proteins bound by antibodies in sera from animals with experimental autoimmune uveitis and uveitis patients., PMID:6863917

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

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Distributor list

For research use only. Not for human or drug use.

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Anti-Human GNAT1 Polyclonal Antibody [PHC89101]
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