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Anti-Human ROM1 Polyclonal Antibody

Catalog #:   PHF98901 Specific References (12) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: Q03395
Overview

Catalog No.

PHF98901

Species reactivity

Human

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human ROM1 (Gly127-Asp263).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

Tspan-23, Rod outer segment membrane protein 1, TSPAN23, ROSP1, Tetraspanin-23, ROM1

Purification

Purified by antigen affinity column.

Accession

Q03395

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • Western Blot
    Various lysates were subjected to SDS PAGE followed by western blot with ROM1 antibody (PHF98901) at 1μg/ml.

    Lane 1: Mouse eye lysate
    Lane 2: Rat eye lysate

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 38 kDa
    Observed MW: 42 kDa
  • Immunohistochemical
    Immunohistochemical analysis of human retina tissue stained for ROM1 with PHF98901.
  • Western Blot
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with ROM1 antibody (PHF98901) at 1 µg/ml.

    Lane 1: Recombinant Protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 18 kDa
    Observed MW: 18 kDa
References

Protective efficacy induced by Eimeria maxima rhomboid-like protein 1 against homologous infection., PMID:36686197

Nano-scale resolution of native retinal rod disk membranes reveals differences in lipid composition., PMID:34132745

Toxoplasma gondii rhomboid protein 1 (TgROM1) is a potential vaccine candidate against toxoplasmosis., PMID:21906881

A battery of cell- and structure-specific markers for the adult porcine retina., PMID:20086234

Characterization of peripherin/rds and rom-1 transport in rod photoreceptors of transgenic and knockout animals., PMID:16639027

Expression of cone-photoreceptor-specific antigens in a cell line derived from retinal tumors in transgenic mice., PMID:14985288

Proapoptotic bcl-2 family members, Bax and Bak, are essential for developmental photoreceptor apoptosis., PMID:12882813

Deletional analysis of the rod photoreceptor cell peripherin/RDS carboxy-terminal region., PMID:12137760

Expression and characterization of peripherin/rds-rom-1 complexes and mutants implicated in retinal degenerative diseases., PMID:10800708

Three homologs of rds/peripherin in Xenopus laevis photoreceptors that exhibit covalent and non-covalent interactions., PMID:8923216

Molecular cloning, membrane topology, and localization of bovine rom-1 in rod and cone photoreceptor cells., PMID:8603840

[Molecular cloning of the genes in genetic chorioretinal diseases--positional cloning and the candidate gene approach]., PMID:8285146

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

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Distributor list

For research use only. Not for human or drug use.

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Anti-Human ROM1 Polyclonal Antibody [PHF98901]
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