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Anti-Human ATP1B1 Polyclonal Antibody

Catalog #:   PHC12201 Specific References (15) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: P05026
Overview

Catalog No.

PHC12201

Species reactivity

Human

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human ATP1B1 (Gly76-Ser303).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

Sodium/potassium-transporting ATPase subunit beta-1, ATP1B1, Sodium/potassium-dependent ATPase subunit beta-1, ATP1B

Purification

Purified by antigen affinity column.

Accession

P05026

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • Western Blot
    Various lysates were subjected to SDS PAGE followed by western blot with ATP1B1 antibody (PHC12201) at 1μg/ml.

    Lane 1: Mouse brain lysate
    Lane 2: Rat brain lysate

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 35 kDa
    Observed MW: 45 kDa
  • Immunohistochemical
    Immunohistochemical analysis of mouse kidney stained for ATP1B1 with PHC12201.
  • Western Blot
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with ATP1B1 antibody (PHC12201) at 1 µg/ml.

    Lane 1: Recombinant Protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 29 kDa
    Observed MW: 29 kDa
References

Zenocutuzumab, a HER2xHER3 Bispecific Antibody, Is Effective Therapy for Tumors Driven by NRG1 Gene Rearrangements., PMID:35135829

NRG1 fusion-driven tumors: biology, detection, and the therapeutic role of afatinib and other ErbB-targeting agents., PMID:32916265

Immunohistochemical validation study of 15-gene biomarker panel predictive of benefit from adjuvant chemotherapy in resected non-small-cell lung cancer: analysis of JBR.10., PMID:32220948

Regulation of Na,K-ATPase β1-subunit in TGF-β2-mediated epithelial-to-mesenchymal transition in human retinal pigmented epithelial cells., PMID:23810808

Identification of the amino acid region involved in the intercellular interaction between the β1 subunits of Na+/K+ -ATPase., PMID:22328500

Allosteric property of the (Na⁺+K⁺)-ATPase β₁ subunit., PMID:22056556

A C-terminal lobe of the beta subunit of Na,K-ATPase and H,K-ATPase resembles cell adhesion molecules., PMID:19694409

Fast degradation of the auxiliary subunit of Na+/K+-ATPase in the plasma membrane of HeLa cells., PMID:18522992

Oral polyamine administration modifies the ontogeny of hexose transporter gene expression in the postnatal rat intestine., PMID:17673438

Na/K-ATPase beta1 subunit expression is required for blastocyst formation and normal assembly of trophectoderm tight junction-associated proteins., PMID:17317668

A carboxy-terminus motif of HKalpha2 is necessary for assembly and function., PMID:15569317

Identification of a prostaglandin-responsive element in the Na,K-ATPase beta 1 promoter that is regulated by cAMP and Ca2+. Evidence for an interactive role of cAMP regulatory element-binding protein and Sp1., PMID:15485816

The effect of beta-subunit assembly on function and localization of the colonic H+,K+-ATPase alpha-subunit., PMID:15327400

The carboxy terminus of the colonic H(+), K(+)-ATPase alpha-subunit is required for stable beta subunit assembly and function., PMID:15086469

The gamma-Na+,K+-ATPase subunit assembles selectively with alpha1/beta1-Na+,K+-ATPase but not with the colonic H+,K+-ATPase., PMID:11849451

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

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For research use only. Not for human or drug use.

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Anti-Human ATP1B1 Polyclonal Antibody [PHC12201]
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