Catalog No.
PHG76101
Species reactivity
Human
Host species
Rabbit
Isotype
IgG
Clonality
Polyclonal
Immunogen
E. coli - derived recombinant Human EIF2B1 (Met1-Leu305).
Tested applications
ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000
Target
Translation initiation factor eIF-2B subunit alpha, eIF-2B GDP-GTP exchange factor subunit alpha, EIF2BA, EIF2B1
Purification
Purified by antigen affinity column.
Accession
Q14232
Applications
ELISA, IHC, WB
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Integrative analysis of serum proteomics and transcriptomics in hepatitis C., PMID:40082995
Interstitial lung disease in a patient with anti-eIF2B antibodies-positive systemic sclerosis: A case report and literature review., PMID:39654607
ELISA detection of anti-eIF2B antibodies in Japanese patients with systemic sclerosis., PMID:38048623
Detection of anti-eIF2B autoantibodies in systemic sclerosis by immunoprecipitation-mass spectrometry., PMID:36715621
Role of autoantibodies in the diagnosis and prognosis of interstitial lung disease in autoimmune rheumatic disorders., PMID:34377160
Autoantibodies as biomarkers for interstitial lung disease in idiopathic inflammatory myositis and systemic sclerosis: The case of anti-eIF2B antibodies., PMID:32743530
Saccharomyces cerevisiae ER membrane protein complex subunit 4 (EMC4) plays a crucial role in eIF2B-mediated translation regulation and survival under stress conditions., PMID:32476094
Anti-nuclear autoantibodies in systemic sclerosis : News and perspectives., PMID:35382013
Analysis of the Antigenic and Prophylactic Properties of the Leishmania Translation Initiation Factors eIF2 and eIF2B in Natural and Experimental Leishmaniasis., PMID:29675401
Presence of anti-eukaryotic initiation factor-2B, anti-RuvBL1/2 and anti-synthetase antibodies in patients with anti-nuclear antibody negative systemic sclerosis., PMID:29294089
Role of Saccharomyces cerevisiae TAN1 (tRNA acetyltransferase) in eukaryotic initiation factor 2B (eIF2B)-mediated translation control and stress response., PMID:28677085
Real-Time Reverse-Transcription Quantitative Polymerase Chain Reaction Assay Is a Feasible Method for the Relative Quantification of Heregulin Expression in Non-Small Cell Lung Cancer Tissue., PMID:28469400
Brief Report: Anti-Eukaryotic Initiation Factor 2B Autoantibodies Are Associated With Interstitial Lung Disease in Patients With Systemic Sclerosis., PMID:27273608
Association of eukaryotic translation initiation factor eIF2B with fully solubilized CXCR4., PMID:26609049
A cellular assay for measuring the inhibition of glycogen synthase kinase-3 via the accumulation of beta-catenin in Chinese hamster ovary clone K1 cells., PMID:16945017
Dynamic cycling of eIF2 through a large eIF2B-containing cytoplasmic body: implications for translation control., PMID:16157703
The life and death of oligodendrocytes in vanishing white matter disease., PMID:15217090
Phosphorylation of eukaryotic translation initiation factor 2Bepsilon by glycogen synthase kinase-3beta regulates beta-adrenergic cardiac myocyte hypertrophy., PMID:15001529
The regulation of protein synthesis and translation factors by CD3 and CD28 in human primary T lymphocytes., PMID:12028592
Serine 48 in initiation factor 2 alpha (eIF2 alpha) is required for high-affinity interaction between eIF2 alpha(P) and eIF2B., PMID:10563826
Increased activity of eukaryotic initiation factor 2B in PC12 cells in response to differentiation by nerve growth factor., PMID:9798914
Complex formation by positive and negative translational regulators of GCN4., PMID:2038327
For research use only. Not for human or drug use.
