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Anti-Human YWHAE Polyclonal Antibody

Catalog #:   PHF49301 Specific References (17) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: P62258
Overview

Catalog No.

PHF49301

Species reactivity

Human

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human YWHAE (Met1-Gln255).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

14-3-3E, YWHAE, 14-3-3 protein epsilon

Purification

Purified by antigen affinity column.

Accession

P62258

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • Western Blot
    Various lysates were subjected to SDS PAGE followed by western blot with YWHAE antibody (PHF49301) at 1μg/ml.

    Lane 1: Hela cell lysate
    Lane 2: MCF-7 cell lysate
    Lane 3: Jurkat cell lysate
    Lane 4: NIH3T3 cell lysate
    Lane 5: RAW264.7 cell lysate
    Lane 6: A375 cell lysate
    Lane 7: 293T cell lysate

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 29 kDa
    Observed MW: 29 kDa
  • Immunohistochemical
    Immunohistochemical analysis of human cerebral cortex tissue stained for YWHAE with PHF49301.
  • Western Blot
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with YWHAE antibody (PHF49301) at 1 µg/ml.

    Lane 1: Recombinant Protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 31 kDa
    Observed MW: 31 kDa
References

AGR2-mediated unconventional secretion of 14-3-3ε and α-actinin-4, responsive to ER stress and autophagy, drives chemotaxis in canine mammary tumor cells., PMID:38822246

Listening to the Whispers in Neuroimmune Crosstalk: A Comprehensive Workflow to Investigate Neurotrophin Receptor p75NTR Under Endogenous, Low Abundance Conditions., PMID:33936068

Immunophenotype-Genotype Correlations in Clear Cell Sarcoma of Kidney-An Evaluation of Diagnostic Ancillary Studies., PMID:32364435

Activation of innate immunity by 14-3-3 ε, a new potential alarmin in osteoarthritis., PMID:32173627

An Efficient Exogenous Gene Insertion Site in CHO Cells with High Transcription Level to Enhance AID-Induced Mutation., PMID:31975519

Functional study of 14-3-3 protein epsilon (YWHAE) in keratinocytes: microarray integrating bioinformatics approaches., PMID:31244373

A recombinant Fasciola gigantica 14-3-3 epsilon protein (rFg14-3-3e) modulates various functions of goat peripheral blood mononuclear cells., PMID:29510740

Dysregulated mitogen-activated protein kinase signalling as an oncogenic basis for clear cell sarcoma of the kidney., PMID:29243812

Clear cell sarcomas of the kidney are characterised by BCOR gene abnormalities, including exon 15 internal tandem duplications and BCOR-CCNB3 gene fusion., PMID:28833375

BCOR Overexpression Is a Highly Sensitive Marker in Round Cell Sarcomas With BCOR Genetic Abnormalities., PMID:27428733

The pro-inflammatory cytokine 14-3-3ε is a ligand of CD13 in cartilage., PMID:26208633

Expression of ROS1 predicts ROS1 gene rearrangement in inflammatory myofibroblastic tumors., PMID:25612511

Molecular characterization of a population-based series of endometrial stromal sarcomas in Kuwait., PMID:25288234

Frequent expression of KIT in endometrial stromal sarcoma with YWHAE genetic rearrangement., PMID:24186140

YWHAE rearrangement identified by FISH and RT-PCR in endometrial stromal sarcomas: genetic and pathological correlations., PMID:23599159

High levels of immunoglobulin E autoantibody to 14-3-3 epsilon protein correlate with protection against severe Plasmodium falciparum malaria., PMID:22984113

A systems biology approach to identify the signalling network regulated by Rho-GDI-γ during neural stem cell differentiation., PMID:22892720

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

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For research use only. Not for human or drug use.

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Anti-Human YWHAE Polyclonal Antibody [PHF49301]
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