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Anti-Human ME1 Polyclonal Antibody

Catalog #:   PHE56101 Specific References (50) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: P48163
Overview

Catalog No.

PHE56101

Species reactivity

Human

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human ME1 (Ser50-Lys525).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

ME1, NADP-dependent malic enzyme, NADP-ME, Malic enzyme 1

Purification

Purified by antigen affinity column.

Accession

P48163

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • WESTERN-BLOT
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with ME1 antibody (PHE56101) at 1 μg/ml.

    Lane 1: Recombinant protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 55 kDa
    Observed MW: 55 kDa
  • Western Blot
    Various lysates were subjected to SDS PAGE followed by western blot with ME1 antibody (PHE56101) at 1 μg/ml.

    Lane 1: A431
    Lane 2: Hela

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 64 kDa
    Observed MW: 64 kDa
  • Immunohistochemical
    Immunohistochemical analysis of mouse testis tissue stained for ME1 with PHE56101.
References

Safety and efficacy of pembrolizumab, radiation therapy, and surgery versus radiation therapy and surgery for stage III soft tissue sarcoma of the extremity (SU2C-SARC032): an open-label, randomised clinical trial., PMID:39547252

Respiratory disease complex due to mixed viral infections in chicken in Jordan., PMID:38417340

USP19 exacerbates lipogenesis and colorectal carcinogenesis by stabilizing ME1., PMID:34965422

Phase II Randomized Study of CMB305 and Atezolizumab Compared With Atezolizumab Alone in Soft-Tissue Sarcomas Expressing NY-ESO-1., PMID:34260265

Interim Analysis of the Phase II Study: Noninferiority Study of Doxorubicin with Upfront Dexrazoxane plus Olaratumab for Advanced or Metastatic Soft-Tissue Sarcoma., PMID:33766818

A liposomal RNA vaccine inducing neoantigen-specific CD4+ T cells augments the antitumor activity of local radiotherapy in mice., PMID:32923128

Evaluation in broilers of aerosolized nanoparticles vaccine encapsulating imuno-stimulant and antigens of avian influenza virus/Mycoplasma gallisepticum., PMID:32867774

Malic enzyme 1 is important for uterine decidualization in response to progesterone/cAMP/PKA/HB-EGF pathway., PMID:31944402

Opposing Effects of PPARα Agonism and Antagonism on Refractive Development and Form Deprivation Myopia in Guinea Pigs., PMID:30521668

Examining the Roles of H3K4 Methylation States with Systematically Characterized Antibodies., PMID:30244833

The DNA methylation landscape of enhancers in the guinea pig hippocampus., PMID:29616589

Antibody-mediated inhibition of EGFR reduces phosphate excretion and induces hyperphosphatemia and mild hypomagnesemia in mice., PMID:28292888

Inhibition of H3K9 histone methyltransferase G9a attenuates renal fibrosis and retains klotho expression., PMID:26444031

Critical role of endoplasmic reticulum aminopeptidase 1 in determining the length and sequence of peptides bound and presented by HLA-B27., PMID:24504800

Expression of aberrant HLA-B27 molecules is dependent on B27 dosage and peptide supply., PMID:23625978

[Preparation of a monoclonal antibody against fusion protein preM/EIII of WNV]., PMID:22588939

A novel combined vaccine candidate containing epitopes of HCV NS3, core and E1 proteins induces multi-specific immune responses in BALB/c mice., PMID:19646486

Tumor necrosis factor-alpha and interleukin-1 antagonists alleviate inflammatory skin changes associated with epidermal growth factor receptor antibody therapy in mice., PMID:19584274

Two HLA-B27 alleles differently associated with spondylarthritis, B*2709 and B*2705, display similar intracellular trafficking and oligomer formation., PMID:17599742

The amino acid at position 97 is involved in folding and surface expression of HLA-B27., PMID:16361312

Single chain antibody against the common epitope of mutant p53 restores wild-type activity to mutant p53 protein., PMID:16213502

Bacterial expression and enzymatic activity analysis of ME1, a ribosome-inactivating protein from Mirabilis expansa., PMID:15721782

Uniquely conformed peptide-containing beta 2-microglobulin-free heavy chains of HLA-B2705 on the cell surface., PMID:12370371

The genetically-engineered secretory B27/Q10 chimeric molecule inhibits HLA-B27 restricted alloreactive T-lymphocytes., PMID:12175099

Single-chain antibody against the common epitope of mutant p53: isolation and intracytosolic expression in mammalian cells., PMID:11684134

The recognition of HLA-B27 by human CD4(+) T lymphocytes., PMID:11509603

Biosynthesis of ribosome-inactivating proteins from callus and cell suspension cultures of Mirabilis expansa (Ruiz &Pavon)., PMID:30754835

Primary structure, genomic organization and expression of the major secretory protein of murine epididymis, ME1., PMID:10863096

Dependence of CD8+ T-cell-mediated suppression of HIV type 1 on viral phenotypes and mediation of phenotype-dependent suppression by viral envelope gene and not by beta-chemokines., PMID:10659051

Phenotypic and functional characteristics of porcine peritoneal mesothelial cells., PMID:10614873

Biotinylation of class I MHC molecules abrogates recognition by W6/32 antibody., PMID:10395109

Cutting edge: HLA-B27 can form a novel beta 2-microglobulin-free heavy chain homodimer structure., PMID:10227970

Highly conserved Neisseria meningitidis surface protein confers protection against experimental infection., PMID:9104804

Interleukin 1alpha and interleukin 6 promote the in vitro growth of both normal and neoplastic human cervical epithelial cells., PMID:9062407

Specificity of two anti-class I HLA monoclonal antibodies that block class I recognition by the NKB1 killer cell inhibitory receptor., PMID:8946681

A comparison of immunohistochemical staining of human cultured mesothelial cells and ovarian tumour cells using epithelial and mesothelial cell markers., PMID:8888953

Expression of platelet-derived growth factor (PDGF) and PDGF receptors in human malignant mesothelioma in vitro and in vivo., PMID:8683381

Catabolism of radioiodinated murine monoclonal antibody F(ab')2 fragment labeled using N-succinimidyl 3-iodobenzoate and Iodogen methods., PMID:7578370

The subtilisins of the invertebrate mycopathogens Verticillium chlamydosporium and Metarhizium anisopliae are serologically and functionally related., PMID:7729666

Phase I studies of treatment of malignant gliomas and neoplastic meningitis with 131I-radiolabeled monoclonal antibodies anti-tenascin 81C6 and anti-chondroitin proteoglycan sulfate Me1-14 F (ab')2--a preliminary report., PMID:8523067

ME1-antibody labelling of primary bronchogenic tumours and extrapulmonary malignancies., PMID:7529122

Mouse/human chimeric Me1-14 antibody: genomic cloning of the variable region genes, linkage to human constant region genes, expression, and characterization., PMID:8050781

Immunocytochemical characterization of cell lines from human malignant mesothelioma: characterization of human mesothelioma cell lines by immunocytochemistry with a panel of monoclonal antibodies., PMID:7512071

A monoclonal antibody that recognizes HLA-B27 in the context of peptides., PMID:8301124

Analysis of HLA-B*2705 peptide motif, using T2 cells and monoclonal antibody ME1., PMID:7910590

HLA-B67: a member of the HLA-B16 family that expresses the ME1 epitope., PMID:7517584

Basic fibroblast growth factor induces differentiation of neural tube and neural crest lineages of cultured ectoderm cells from Xenopus gastrula., PMID:8306875

HLA-B*0702 antibody epitopes are affected indirectly by distant antigen residues., PMID:7681814

In vitro mutagenesis of HLA-B27: single and multiple amino acid substitutions at consensus B27 sites identify distinct monoclonal antibody-defined epitopes., PMID:1379218

Inductive differentiation of two neural lineages reconstituted in a microculture system from Xenopus early gastrula cells., PMID:1769329

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

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Anti-Human ME1 Polyclonal Antibody [PHE56101]
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