Please ensure Javascript is enabled for purposes of website accessibility
Home / Products / Antibody / Polyclonal Antibody

Anti-Human ME1 Polyclonal Antibody

Catalog #:   PHE56101 Specific References (50) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: P48163
Overview

Catalog No.

PHE56101

Species reactivity

Human

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human ME1 (Ser50-Lys525).

Applications

ELISA, IHC, WB

Recommended Dilution

Application Dilution
ELISA 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

ME1, NADP-dependent malic enzyme, NADP-ME, Malic enzyme 1

Purification

Purified by antigen affinity column.

Accession

P48163

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Note

For research use only.

Data Image
  • WESTERN-BLOT
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with ME1 antibody (PHE56101) at 1 μg/ml.

    Lane 1: Recombinant protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 55 kDa
    Observed MW: 55 kDa
  • Western Blot
    Various lysates were subjected to SDS PAGE followed by western blot with ME1 antibody (PHE56101) at 1 μg/ml.

    Lane 1: A431
    Lane 2: Hela

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 64 kDa
    Observed MW: 64 kDa
  • Immunohistochemical
    Immunohistochemical analysis of mouse testis tissue stained for ME1 with PHE56101.
References

Inductive differentiation of two neural lineages reconstituted in a microculture system from Xenopus early gastrula cells., PMID:1769329

In vitro mutagenesis of HLA-B27: single and multiple amino acid substitutions at consensus B27 sites identify distinct monoclonal antibody-defined epitopes., PMID:1379218

HLA-B*0702 antibody epitopes are affected indirectly by distant antigen residues., PMID:7681814

Basic fibroblast growth factor induces differentiation of neural tube and neural crest lineages of cultured ectoderm cells from Xenopus gastrula., PMID:8306875

HLA-B67: a member of the HLA-B16 family that expresses the ME1 epitope., PMID:7517584

Analysis of HLA-B*2705 peptide motif, using T2 cells and monoclonal antibody ME1., PMID:7910590

A monoclonal antibody that recognizes HLA-B27 in the context of peptides., PMID:8301124

Immunocytochemical characterization of cell lines from human malignant mesothelioma: characterization of human mesothelioma cell lines by immunocytochemistry with a panel of monoclonal antibodies., PMID:7512071

Mouse/human chimeric Me1-14 antibody: genomic cloning of the variable region genes, linkage to human constant region genes, expression, and characterization., PMID:8050781

ME1-antibody labelling of primary bronchogenic tumours and extrapulmonary malignancies., PMID:7529122

Phase I studies of treatment of malignant gliomas and neoplastic meningitis with 131I-radiolabeled monoclonal antibodies anti-tenascin 81C6 and anti-chondroitin proteoglycan sulfate Me1-14 F (ab')2--a preliminary report., PMID:8523067

The subtilisins of the invertebrate mycopathogens Verticillium chlamydosporium and Metarhizium anisopliae are serologically and functionally related., PMID:7729666

Catabolism of radioiodinated murine monoclonal antibody F(ab')2 fragment labeled using N-succinimidyl 3-iodobenzoate and Iodogen methods., PMID:7578370

Expression of platelet-derived growth factor (PDGF) and PDGF receptors in human malignant mesothelioma in vitro and in vivo., PMID:8683381

A comparison of immunohistochemical staining of human cultured mesothelial cells and ovarian tumour cells using epithelial and mesothelial cell markers., PMID:8888953

Specificity of two anti-class I HLA monoclonal antibodies that block class I recognition by the NKB1 killer cell inhibitory receptor., PMID:8946681

Interleukin 1alpha and interleukin 6 promote the in vitro growth of both normal and neoplastic human cervical epithelial cells., PMID:9062407

Highly conserved Neisseria meningitidis surface protein confers protection against experimental infection., PMID:9104804

Cutting edge: HLA-B27 can form a novel beta 2-microglobulin-free heavy chain homodimer structure., PMID:10227970

Biotinylation of class I MHC molecules abrogates recognition by W6/32 antibody., PMID:10395109

Phenotypic and functional characteristics of porcine peritoneal mesothelial cells., PMID:10614873

Dependence of CD8+ T-cell-mediated suppression of HIV type 1 on viral phenotypes and mediation of phenotype-dependent suppression by viral envelope gene and not by beta-chemokines., PMID:10659051

Primary structure, genomic organization and expression of the major secretory protein of murine epididymis, ME1., PMID:10863096

Biosynthesis of ribosome-inactivating proteins from callus and cell suspension cultures of Mirabilis expansa (Ruiz &Pavon)., PMID:30754835

The recognition of HLA-B27 by human CD4(+) T lymphocytes., PMID:11509603

Single-chain antibody against the common epitope of mutant p53: isolation and intracytosolic expression in mammalian cells., PMID:11684134

The genetically-engineered secretory B27/Q10 chimeric molecule inhibits HLA-B27 restricted alloreactive T-lymphocytes., PMID:12175099

Uniquely conformed peptide-containing beta 2-microglobulin-free heavy chains of HLA-B2705 on the cell surface., PMID:12370371

Bacterial expression and enzymatic activity analysis of ME1, a ribosome-inactivating protein from Mirabilis expansa., PMID:15721782

Single chain antibody against the common epitope of mutant p53 restores wild-type activity to mutant p53 protein., PMID:16213502

The amino acid at position 97 is involved in folding and surface expression of HLA-B27., PMID:16361312

Two HLA-B27 alleles differently associated with spondylarthritis, B*2709 and B*2705, display similar intracellular trafficking and oligomer formation., PMID:17599742

Tumor necrosis factor-alpha and interleukin-1 antagonists alleviate inflammatory skin changes associated with epidermal growth factor receptor antibody therapy in mice., PMID:19584274

A novel combined vaccine candidate containing epitopes of HCV NS3, core and E1 proteins induces multi-specific immune responses in BALB/c mice., PMID:19646486

[Preparation of a monoclonal antibody against fusion protein preM/EIII of WNV]., PMID:22588939

Expression of aberrant HLA-B27 molecules is dependent on B27 dosage and peptide supply., PMID:23625978

Critical role of endoplasmic reticulum aminopeptidase 1 in determining the length and sequence of peptides bound and presented by HLA-B27., PMID:24504800

Inhibition of H3K9 histone methyltransferase G9a attenuates renal fibrosis and retains klotho expression., PMID:26444031

Antibody-mediated inhibition of EGFR reduces phosphate excretion and induces hyperphosphatemia and mild hypomagnesemia in mice., PMID:28292888

The DNA methylation landscape of enhancers in the guinea pig hippocampus., PMID:29616589

Examining the Roles of H3K4 Methylation States with Systematically Characterized Antibodies., PMID:30244833

Opposing Effects of PPARα Agonism and Antagonism on Refractive Development and Form Deprivation Myopia in Guinea Pigs., PMID:30521668

Malic enzyme 1 is important for uterine decidualization in response to progesterone/cAMP/PKA/HB-EGF pathway., PMID:31944402

Evaluation in broilers of aerosolized nanoparticles vaccine encapsulating imuno-stimulant and antigens of avian influenza virus/Mycoplasma gallisepticum., PMID:32867774

A liposomal RNA vaccine inducing neoantigen-specific CD4+ T cells augments the antitumor activity of local radiotherapy in mice., PMID:32923128

Interim Analysis of the Phase II Study: Noninferiority Study of Doxorubicin with Upfront Dexrazoxane plus Olaratumab for Advanced or Metastatic Soft-Tissue Sarcoma., PMID:33766818

Phase II Randomized Study of CMB305 and Atezolizumab Compared With Atezolizumab Alone in Soft-Tissue Sarcomas Expressing NY-ESO-1., PMID:34260265

USP19 exacerbates lipogenesis and colorectal carcinogenesis by stabilizing ME1., PMID:34965422

Respiratory disease complex due to mixed viral infections in chicken in Jordan., PMID:38417340

Safety and efficacy of pembrolizumab, radiation therapy, and surgery versus radiation therapy and surgery for stage III soft tissue sarcoma of the extremity (SU2C-SARC032): an open-label, randomised clinical trial., PMID:39547252

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

Contact Information

Order: order@antibodysystem.com

Mail: support@antibodysystem.com

Distributor list

For research use only. Not for human or drug use.

Need help with your order?

Find out more about placing an order here

Anti-Human ME1 Polyclonal Antibody [PHE56101]
Terms of sale Website terms of use Cookie policy Privacy
Copyright © 2025 AntibodySystem SAS. All Rights Reserved.            All Products are for Research Use Only