Anti-Human LIG4 Polyclonal Antibody

Catalog No.

PHE73701

Species reactivity

Human

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human LIG4 (Met1-Lys609).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

DNA ligase IV, Polydeoxyribonucleotide synthase [ATP] 4, LIG4, DNA ligase 4

Purification

Purified by antigen affinity column.

Accession

P49917

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

DNA Ligase 4 Inhibition Sensitizes Prostate Cancer to Immune Checkpoint Blockade In Vivo., PMID:40037856

DNA ligase IV dificiency with elevated serum IgG levels suspected to have myelodysplastic syndrome: a case report., PMID:36221079

Multiple DSB Resection Activities Redundantly Promote Alternative End Joining-Mediated Class Switch Recombination., PMID:34926456

X-ray scattering reveals disordered linkers and dynamic interfaces in complexes and mechanisms for DNA double-strand break repair impacting cell and cancer biology., PMID:34056803

Precision medicine phase II study evaluating the efficacy of a double immunotherapy by durvalumab and tremelimumab combined with olaparib in patients with solid cancers and carriers of homologous recombination repair genes mutation in response or stable after olaparib treatment., PMID:32778095

Genetic interaction between the non-homologous end-joining factors during B and T lymphocyte development: In vivo mouse models., PMID:32654175

Intra-Vκ Cluster Recombination Shapes the Ig Kappa Locus Repertoire., PMID:31875554

Generation of a Mouse Model Lacking the Non-Homologous End-Joining Factor Mri/Cyren., PMID:31795137

Effects of DNA end configuration on XRCC4-DNA ligase IV and its stimulation of Artemis activity., PMID:28696258

XLF deficiency results in reduced N-nucleotide addition during V(D)J recombination., PMID:27281794

The DNA Ligase IV Syndrome R278H Mutation Impairs B Lymphopoiesis via Error-Prone Nonhomologous End-Joining., PMID:26608917

Organization and dynamics of the nonhomologous end-joining machinery during DNA double-strand break repair., PMID:25941401

Phosphorylated Sp1 is the regulator of DNA-PKcs and DNA ligase IV transcription of daunorubicin-resistant leukemia cell lines., PMID:24530422

ID1 affects the efficacy of radiotherapy in glioblastoma through inhibition of DNA repair pathways., PMID:23377983

Arabidopsis ARP endonuclease functions in a branched base excision DNA repair pathway completed by LIG1., PMID:21781197

Competition between PARP-1 and Ku70 control the decision between high-fidelity and mutagenic DNA repair., PMID:21256093

Widespread dependence of backup NHEJ on growth state: ramifications for the use of DNA-PK inhibitors., PMID:20950945

Alternative end-joining catalyzes class switch recombination in the absence of both Ku70 and DNA ligase 4., PMID:20142431

Homozygous DNA ligase IV R278H mutation in mice leads to leaky SCID and represents a model for human LIG4 syndrome., PMID:20133615

Inter-individual variation in DNA double-strand break repair in human fibroblasts before and after exposure to low doses of ionizing radiation., PMID:19896956

Association between total immunoglobulin E and antibody responses to naturally acquired Ascaris lumbricoides infection and polymorphisms of immune system-related LIG4, TNFSF13B and IRS2 genes., PMID:19604268

Impaired lymphocyte development and antibody class switching and increased malignancy in a murine model of DNA ligase IV syndrome., PMID:19451691

The human set and transposase domain protein Metnase interacts with DNA Ligase IV and enhances the efficiency and accuracy of non-homologous end-joining., PMID:18773976

Human DNA ligases I and III, but not ligase IV, are required for microhomology-mediated end joining of DNA double-strand breaks., PMID:18440984

IgH class switching and translocations use a robust non-classical end-joining pathway., PMID:17713479

A severe form of human combined immunodeficiency due to mutations in DNA ligase IV., PMID:16585603

Severe combined immunodeficiency and microcephaly in siblings with hypomorphic mutations in DNA ligase IV., PMID:16358361

Both V(D)J coding ends but neither signal end can recombine at the bcl-2 major breakpoint region, and the rejoining is ligase IV dependent., PMID:16024785

Impact of DNA ligase IV on nonhomologous end joining pathways during class switch recombination in human cells., PMID:15657289

A Rad50-dependent pathway of DNA repair is deficient in Fanconi anemia fibroblasts., PMID:15199173

Biochemical evidence for Ku-independent backup pathways of NHEJ., PMID:12954774

In vitro and in vivo interactions of DNA ligase IV with a subunit of the condensin complex., PMID:12589063

Identification of human autoantibodies to the DNA ligase IV/XRCC4 complex and mapping of an autoimmune epitope to a potential regulatory region., PMID:12218164

Autoantibodies against DNA double-strand break repair proteins., PMID:11689355

Genetic evidence for the involvement of DNA ligase IV in the DNA-PK-dependent pathway of non-homologous end joining in mammalian cells., PMID:11292837

Late embryonic lethality and impaired V(D)J recombination in mice lacking DNA ligase IV., PMID:9823897

DNA ligase IV from HeLa cell nuclei., PMID:8798671

Monoclonal antibodies recognising differentiation antigens on porcine B cells., PMID:7856057

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